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Study on the gene abnormalities that cause glucocorticoid-resistant POMC gene expression in corticotroph adenoma cells Cushing's disease

Research Project

Project/Area Number 09671016
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionHirosaki University

Principal Investigator

SUDA Toshihiro  Hirosaki University School of Medicine, Department of Medicine, Professor, 医学部, 教授 (30075452)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCushing's disease / Pituitary adenoma / POMC / protooncogene / gene abnormality / CRF / CRF receptor / プロオピオメラノコルチン / プロトオンコジーン
Research Abstract

To clarity the mechanism of hypersecretion of ACTH and the resistance of ACTH secretion to glucocorticoid, (1) gene expression of c-fos, (2) gene expression of CRF-R1, (3) response of ACTH to DDAVP and characterization of Vi b mRNA were studied in ACTH-producing pituitary adenoma cells.
(1) c-f os Gene expression of c-fos in corticotroph adenoma cells was greater than that in non-adenoma cells. There were 2 point mutations (somatic mutation) at 5'-UTR in c-fos gene. Fos seems to bind to OR and inhibit OR-induced suppression of POMG gene expression. In addition, Fos may stimulate POMO gene expression.
(2) CRF-R1 mRNA levels were down-regulated by CRF in corticotrophs, but were up-regulated by CRF in corticotroph adenoma cells. These CRF-induced down- and up-regulation of CRF-R1 mRNA levels were mediated by cAMP.VP and glucocorticoid down-regulated CRF-RI mRNA levels in both types of ACTH cells.
(3) DDAVP, V2-agonist, stimulated ACTH secretion from corticotroph adenoma cells, but did not stimulate it in normal ACTH cells. Vib mRNA levels in corticotroph adenoma cells increased after stimulation of VP and CRF.The size of Vib mRNA in adenoma cells was similar to that of authentic V1 b mRNA.
(4) Saikosaponin and serotonin stimulated GRF gene expression in the paraventricular nucleus of the hypothalamus in rats.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Sakai, Y: "Corticotropin-releasing factor up-regulates its own receptor gene-expression in corticotropic adenoma cells in-vitro" J.Clin.Endocrinol.Metab.82. 1229-1234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tozawa, F.: "Saireito decreases inhibitory effect of prednisolone and accelerates the recovery of rat hypothalamic-pituitary-adrenal axis" Endocrine Journal. 45. 69-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kagayama, K: "Serotunin stimulates corticotropin-releasing factor gene expression in the hypothalamic piraventricular roccleus of conscious rats" Neuroscience Letters. 243. 17-20 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Habu, S: "Different roles of brain IL-I in the ACTH response to central versus peripheral administration of liropolysaccharide in the rat" Cytokine. 10. 390-394 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Watanabe, H: "Evidence that a fast, rate-sensitive negative feedback effect corticosterone is not a principal mechanism underlying the indomethacin inhibition of interleukin-beta-induced adrenocorticotropin secretion in the rat" Cytokine. 10. 377-381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai, K: "The genomic organization of the human corticotropin-releasing factor type-1 receptor" Gene. 219. 125-130 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 須田 俊宏(分担執筆): "内分泌・代謝疾患, 執筆項目「プレクリニカル クッシング症候群」" 中山書店, 419(402-406) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 堀場伸郎, 須田俊宏(分担執筆): "ストレスとホルモン, 執筆項目「プロオピオメラノ, ルチル遺伝子の発現判断」" 学会出版センター, 245(37-50) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Sakai, N.Horiba, T.Suda et al: "CRF up-regulates its own receptor gene expression in corticotropic adenoma cells in vitro." J.Clin.Endocrinol.Metab.82(4). 1229-1234 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Suda: "Preclinical Cushing's syndrome and ACTH-independent bilateral adrenocortical macronodular hyperlasia." Internal Medicine. 36(9). 601-601 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Sakai, N.Horiba, T.Suda et al.: "Desmopressin stimulation test for diagnosis of ACTH-dependent Cushing's syndrome." Endocrine J.44(5). 687-695 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.Tozawa, I.Dobashi, m T.Suda et al.: "Saireito(Chinese herbal drug)decreases inhibitory effect of prednisolone and accelerates the recovery of rat hypothalamic-pituitary-adrenal axis." Endocrine J. 45(1). 69-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kageyama, F.Tozawa, T.Suda et al.: "Serotonin stimutates CRF gene expression in the hypothalamic paraventricular nucleus of conscious rats." Neurisci.Lett. 243. 17-20 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Habu, H.Watanabe, T.Suda, et al.: "Different roles of brain interleukin-1 in the ACTH response to central versus peripheral administration of LPS in the rat." Cytokine. 10(5). 390-394 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Waranobe, R.Nasusita, T.Suda et al.: "Evidence that a fast, rate-sensitive negative feedback effect of corticosterone is not a principal mechanism underlying the interlrukin-1 beta-inducedACTHsecretionintherat." Cytokine. 10(5). 377-381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sakai, M.Yamada, T.suda et al.: "The genomic organization of the human CRF type-1 receptor." Gene. 219. 125-130 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Togawa,F.: "Saireito deveases inhibitory effect of preonicolone and sccalerates the recovery of Lat hypothelamic-pituitacy-adrenal oxis" Endocrine Journal. 45(1). 69-74 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe,H.: "Measurement of cytokine in the cavernous sirus plasme from patients with Cashings disease" Neuro Peptides.32(2). 119-123 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kageyama,K.: "Serotonin stimulates corticotropin-releasing factor gene expression in the hypothelamic paraventricular nucleus of conscious rats" Neuroscience Letters. 243. 17-20 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Habu,S.: "Different roles of brain interleukinal in the adrenocortics tropin responce to control versus peripheral administration of LPS in the rat" Cytokine. 10(5). 390-394 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,K.: "The genomic organization of the human corticotropin-releasing factor type 1-recepter" Gene. 219. 125-130 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe,H.: "Evidence that a fast,rate-sensitive negative feedback effect of corticosterone is not a principal mechanism under lying The indomethacin inhibition of interleukin-BETA-induced adrenocorticotropin secretion in the rat" Cytokine. 10(5). 377-381 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,Y.,: "Corficotropin-releasing factor up-regulates its own receptor gene expression in corticotropic adenoma cells in vitro" J.Clin.Endocrinol.Metab.82・4. 1229-1234 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakai,Y.: "Desmopressin stimulation test for diagnosis of ACTH-Dependent Cushing's syndrome" Endocrine J.44・5. 687-695 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 酒井陽子: "ラット下垂体前葉におけるCRF受容体とPOMCの遺伝子発現に対するCREBの関与" ACTH Related Poptides. 8・1. 31-36 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 酒井 謙: "血漿ACTHが高値を示した続発性副腎不全の一例" ACTH Related Poptides. 8・1. 267-271 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tozawa,F.,: "Saireito (a Chinese herbal drug) decreases inhibitory effect of prednisolone and accelerates the recovery of rat hypothalamic-pitutary-adrenal axis" Endocrine J.45・1. 69-74 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakai,K.,: "The genomic organization of the human corticotropin releasing factor type-1 receptor" Gene. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 堀場伸郎, 須田俊宏(分担執筆): "「ストレスとホルモン」中のプロオピオメラノコルチン(POMC)遺伝子の発現制御" 学会出版センター, 245(37-50) (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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