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STUDIES ON THE EXPRESSION OF THYROTROPIN RECEPTORS IN THYMUS.

Research Project

Project/Area Number 09671024
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionGunma University, School of Medicine

Principal Investigator

MURAKAMI Masami  GUNMA UNIVERSITY SCHOOL OF MEDICINE, FIRST DEPARTMENT OF INTERNAL MEDICINE, INSTRUCTOR, 医学部, 助手 (30241871)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsthyroid / thymus / thyrotropin receptor / iodothyronine deiodinase / thyroid hormone / Graves disease / apoptosis / antithyroid drug / 受容体 / 甲状腺刺激ホルモン / 胸腺上皮細胞 / 脱ヨード酵素 / 甲状腺機能亢進症 / T細胞
Research Abstract

We have reported that thymic hyperplasia is associated with Graves disease that results from the development of antibodies for thyrotropin (TSH) receptors and demonstrated that TSH receptors are expressed in human thymus. Thymic TSH receptors may be involved in the initiation or perpetuation of the autoimmune response, and in the selection of the T cell repertoire that may contribute to the development of autoreactive T cells in Graves disease, as suggested for thymic acetylcholine receptors in myasthenia gravis.
TSH receptor mRNA was expressed in thymic tissue of 5, 10 and 20-day-old and adult rat. TSH receptor mRNA was expressed in cultured rat thymic epithelial cells and TSH stimulated cAMP production in cultured rat thymic epithelial cells in a dose dependent manner, suggesting the expression of functional TSH receptors in thymic epithelial cells. Expression of TSH receptors in rat thymic epithelial cells suggests that thymic TSH receptors may be involved in the initiation or perpet … More uation of the autoimmune response, and in the selection of the T cell repertoire that may contribute to the development of autoreactive T cells in Graves disease. Thymic weight decreased in both thyroidectomized and methimazole (MMI) treated rats in the similar manner. The number of apoptotic cells in thymus increased in thyroidectomized and MMI treated rats. These results suggest that the reduction in thymic size after antithyroid drug treatment observed in patients with Graves' disease may be related, at least in part, to the decrease in circulating thyroid hormone levels.
TィイD24ィエD2, which is the major secretory product of thyroid gland, needs to be converted to TィイD23ィエD2 to exert its biological activity by iodothyronine deiodinases. Type II iodothyronine deiodinase (DII) has a critical role to provide local TィイD23ィエD2. We have demonstrated DII expression in human thymic tissue, suggesting the physiological role of DII in thymus for the effect of thyroid hormones. We have successfully isolated human DII gene to study the regulation mechanisms of DII expression. We have characterized its chromosomal localization and analyzed its promoter region. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Murakami M.et al.: "Gsα mutations in hyperfunctioning thyroid adenomas"Archives of Medical Research. 30. 514-521 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Araki O.,Murakami M.et al.: "Assignment of typeII iodothyronine deiodinase gene (D102) to human chromosome band 14q24.2→q24.3 by in situ hybridization"Cytogenetics and Cell Genetics. 84. 73-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosoi Y.,Murakami M.et al.: "Expression and regulation of type II iodothyronine deiodinase in cultured human skeletal muscle cells"Journal of Clinical Endocrinology and Metabolism. 84. 3293-3300 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamiya Y.,Murakami M.et al.: "Pretranslational regulation of rhythmic type II iodothyronine deiodinase expression by β-adrenergic mechanism in rat pineal gland"Endocrinology. 140. 1272-1278 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosoi Y.,Murakami M.et al.: "Stimulation of Chinese hamster ovary cells expressing human thyrotropin receptors by serum human chorionic gonadotropin of patients with hydatidiform mole"Thyroid. 9. 1205-1210 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Araki O.,Murakami M.et al.: "Northern analysis of type II iodothyronine deiodinase mRNA in rat Harderian gland"Life Sciences. 63. 1843-1848 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 村上正巳、森昌朋: "日本比較内分泌学会編 ホルモンの分子生物学"学会出版センター. 167 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 村上正巳、森昌朋: "最新内科学体系内分泌・代謝疾患"中山書店. 419 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Masami Murakami, Yuji Kamiya, Yasuhiro Yanagita and Masatomo Mori: "Gsα mutations in hyperfunctioning thyroid adenomas."Archives of Medical Research. 30. 514-521 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osamu Araki, Masami Murakami, Tadashi Morimura, Yuji Kamiya, yasuhiro Hosoi, Yukio Kato and Masatomo Mori: "Assignment of type II iodothyronine deiodinase gene (DIO2) to human chromosome band 14q24.2->q24.3 by in situ hybridization."Cytogenetics and Cell Genetics. 84. 73-74 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuhiro hosoi, Masami Murakami, Haruo Mizuma, Takayuki Ogiwara, Makoto Imamura and Masatomo Mori: "Expression and regulation of type II iodothyronine deiodinase in clutured human skeletal muscle cells."Journal of Clinical Endocrinology and Metabolism. 84. 3293-3300 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuji Kamiya, Masami Murakami, Osamu Araki, Yasuhiro Hosoi, Takayuki Ogiwara, Haruo Mizuma and Masatomo Mori: "Pretranslational regulation of rhythmic type II iodothyronine deiodinase expression by β-adrenergic mechanism in the rat pineal gland."Endocrinology. 140. 1272-1278 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuhiro Hosoi, Masami Murakami, Takashi Minegishi, Hiroya Okano, Yoshito Ibuki, Toshiyuki Takeuchi and Masatomo Mori: "Stimulation of Chinese hamster ovary cells expressing human thyrotropin receptors by serum human chorionic gonadotropin of patients with hydatidiform mole."Thyroid. 9. 1205-1210 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osamu Araki, Masami Murakami, Yuji Kamiya, Yasuhiro Hosoi, takayuki Ogiwara, Haruo Mizuma, Tokuji Iriuchijima and Masatomo Mori: "Northern analysis of type II iodothyronine deiodinase mRNA in rat Harderian gland."Life Sciences. 63. 1843-1848 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuji Kamiya, Masami Murakami, Yasuhiro Yanagita, Hirosi Koitabashi, Yukio Nagamachi, Yasuhiro Hosoi, Takayuki Ogiwara, Haruo Mizuma, Tokuji Iriuchijima and Masatomo Mori: "Primary culture of cells from hyperfunctioning thyroid adenoma with an activating mutation of Gαs."Molecular and Cellular Endocrinology. 138. 137-142 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murakami,M .et al: "Gsα mutations in hyperfunctioning thyroid adenomas."Arch. Med. Res.. 30. 514-521 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosoi,Y,Murakami,M. et al.: "Expression and regulation of type II iodo thyronine deiodinase in cultured human skeletal muscle cells"J.Clin.Endocrinol.Metab.. 84. 3293-3300 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Araki,O.,Murakami,M. et al.: "Assignment of type II iodothyronine deiodinase gene (0102) to human chromosome band 14q24.2→q24.3 by insitu hybridization"Cytoqenet.Cell Genet.. 84. 73-74 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kamiya,Y.,Murakami,M. et al.: "Pretranslational regulation of rhythmic type II iodothyronine deiodinase expression by β-adrenergic in the rat pineal gland"Endocrinology. 140. 1272-1278 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hosoi,Y,Murakami,M. et al.: "Stimulation of Chinese hamster ovary cells expressing human thyrotropin receptors by serum human chorionic gonadotropin of patients with hydatidiform mole"Thyroid. 9. 1205-1210 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 村上正也 他: "インターフェロン治療誘発性自己免疫製甲状腺疾患"日本臨床. 57. 1779-1783 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 森昌朋,村上正巳: "内化学"朝倉書店. 7 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 森昌朋,村上正巳: "内化学"文光堂. 5 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] O. Araki et al: "Norehern analysis of type II iodothyronine cleioclinase mRNA in rat Harderian gland." Life Sciences. 63. 1843-1848 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y. Kamiya et al.: "Pretranslational regulation of rhythmic type II iodothyronine : deiodinase edpression by β-aclrenargic mechanism in the rat pineal gland." Endocrinology. 140. 1272-1278 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] O. Araki et al: "Assignment of type II iodothyronine deioclmase gene (D102) to human chromosome band 14q 24.2→q24.3by in situ hybridization." Cytogenet. Cell Genet.in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S. Kakizaki et al.: "HLA anligens in patients with interferon-α-inducecl auloimmune thyroid disorders in chronic hepalitis C." J. Hepatology. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上正巳他: "TSH受容体抗体" ホルモンと臨床. 46. 180-189 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上正巳他: "甲状腺ホルモンの合成と分泌調節の現状" 内分泌・糖尿病科. 7. 9-16 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Kamiya et al.: "Primary culture of cells from hyperfunctioning thyroid aclenoma with an activating mutation of Gas" Molecular and Cellular Endocrinology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 森 昌朋 他: "甲状腺機能異常と胸腺" 内科. 80巻5号. 913-916 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 村上 正己 他: "最新内科学大系 プログレス2 甲状腺疾患の分子医学総論" 中山書店, 7 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 村上 正己 他: "ホルモンの分子生物学4 甲状腺ホルモン甲状腺刺激ホルモン" 学会出版センター, 19 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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