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Expression of SDMF and atherosclerosis

Research Project

Project/Area Number 09671026
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionChiba University

Principal Investigator

MORISAKI Nobuhiro  Chiba Univ.Lecturer, 医学部, 講師 (40174411)

Co-Investigator(Kenkyū-buntansha) SAITO Yasushi  Chiba Univ.Professor, 医学部, 教授 (50101358)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordssmooth muscle cells / SDMF / pheno type / migration / Autocrine / otherosclerosis / オートクリレ
Research Abstract

1. in vivo expression of SDMF : Rats were subjected to baloon catheter injury and SDMF mRNA was examined by the northern blot analysis. SDMF was not expressed in the arteries before injury, but expressed in them 14 days after injury. Moreover, in situ hybridization staining revealed that SDMF mRNA was expressed in the arteries not before injury but 14 days after injury. SDMF was selectively expressed in the thickened intima but not in the media of the arteries 14 days after injury. The expression pattern was similar to that of type I collagen.
2. regulation of SDMF gene : Cultured rat smooth muscle cells were used. Serum depletion from the culture medium stimulated SDMF mRNA expression by the northern blot analysis time-dependently from 12-48h. Next, serum depleted smooth muscle cells were stimulated by serum. On the first day, SDMF mRNA expression markedly decreased, then with increased cell density, SDMF mRNA expression increased, suggesting that cell density affects expression of SDMF gene. Finally, of various cytokines tested, only TGF-beta stimulated expression of SDMF.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Tashiro J, Morisaki N et al: "Administvation of a small amrunt of lard enhances intineal thichening in the baloon catheter injury model without at fectry seram lipids" Scand J Clin Lab Invest. 58. 149-154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kanzaki T, Morisaki N et al: "Role of latent TGF-β binding protein in vascular remodeling" Biocheur Biophys Res Commun. 246. 26-30 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morisaki N et al: "Angisgenic interaction between retina EC and PC from normal unddiabetic rabbits, and phenotype changes of diabetic cells" Cell Mol Biol. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tashiro J,Morisaki N et al.: "administration of a small anmurt of lard enhances intinral thickening in the baloon catheter injury model without affecting serum lipids" Scand J Clin Lab Invest. 58. 149-154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kanzaki T,Morisaki N et al.: "Role of latent TGF-beta binding protein in vascular remodeling" Biochem Biophys Res Commun. 246. 26-30 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morisaki N et al.: "angiogenie interaction between retinal EC and PC from normal and diabetic rabbits, and phenotype changes of diahetic cells" Cell Mol Biol. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tashiro J,Morisaki N et al.: "Administvation of a small amsunt of lard enhances intincel thichening in the baloon catheter injury model without af fecting serum lipids." Scand J Clin Lab Invest. 58. 149-154 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanzaki T,Morisaki N et al.: "Role of latent TGF-β binding protein in vascular remodeling." Biocheur Biophys Res Commun. 246. 26-30 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morisaki N et al.: "Angiogenic intera ction between retlnal EC and PC from mormal and diabetic rabbits, and phenotype chanyes of diabetic cell." Cell Mol Biol. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueng H, Haruno A, Morisaki N et al.: "Local expression of c-type natriupetio peptide marfeedly suppressos neointmal formation vn,rat injured arteries through,an autocrine /paracrine loap" Circulation. 96. 2272-2279 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamura K, …, Morisaki N: "Secretion (5-hydroxytryplanine, 5HT)enhances migration of aortic smooth muscle cells through 5-H-Tz receptors" Atherosclerosis. 132. 139-143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Higashi T, …, Morisaki N et al.: "The receptor for advanced glycation and products mediates the chemotaxis of rabbit smooth muscle cells" Diabetes. 46. 463-472 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kanzaki T, Shiina R, Saito Y, Zardi L, Morisaki N: "Transforming growth factors recept or and fibronction expressions in aortic smooth muscle cells in diabetic rats" Diabetologia. 40. 383-391 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikeda M, …, Morisaki N et al: "Natriuretic peptidl family as a novel antimigration factor of vascular smooth muscle cells" Arterloscler Thromb Vasc Biol. 17. 731-736 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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