• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

In vivo kinetics of atherogenic remnant lipoprotiens

Research Project

Project/Area Number 09671074
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionJIKEI UNIVERSITY SCHOOL of MEDICINE

Principal Investigator

NISHIDE Ryouichi  JIKEI UNIVERSITY SCHOOL of MEDICINE,instructor, 医学部, 助手 (30266663)

Co-Investigator(Kenkyū-buntansha) IKEWAKI Katsunori  JIKEI UNIVERSITY SCHOOL of MEDICINE,LECTURER, 医学部, 講師 (40287199)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsremnant / kinetics / tracer / stable isotope / GC-MS / fractional catabolic rate / production rate / compartmental model / III型高脂血症
Research Abstract

Remnants (chylomicron remnant, VLDL remnant) have been considered to be proatherogenic. However, their exact in vivo metabolism remains unclear. In this study, we performed in vivo kinetic studies using a new labeling technique, namely stable isotopically labeled amino acid method, in type III dyslipidemic patients. D3-Leucine was infused for 12 hours and blood samples were collected frequently during infusion period and followed up to 72 hours. VLDL, IDL, and LDL were isolated by sequential ultracentrifugation and each fraction was further subfractionated into remnant and non-remnant fractions using monoclonal anti-apoB100 antibody. ApoB-100 was isolated by SDSPAGE, followed by hydrolysis and cation exchange chromatography. Tracer/tracee ratio was determined by gas-chromatograph mass-s pectrometry. Tracer/tracee ratios were integrated into multicompartmental model to determine kinetic papameters. As the results, kinetic parameters were not different between remnant and non-remnant fractions. Based on the resuls obtained, remnants are metabolically indistinct from non-remnants in type III patients.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Ikewaki,K.: "ApoA-II kinetics in humans using endogenous labeling with stable isotopes:slower turnover of apoA-II compared with the exogenous radiotracer method." J.Lipid Res.37. 399-407 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikewaki,K.: "Increased catabolic rate of low density lipoproteins in humans with cholesteryl ester transfer protein deficiency" J.Clin.Invest.96. 1573-1581 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikewaki,K.: "Apolipoprotein A-II production rate is a major factor regulating the distribution of apolipoprotien A-1 among HDL subclasses LpA-Iand LPA-1:A-II in normolipidemic humans." Arterioscler.Thromb.Vasc.Biol.15. 306-312 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikewaki,K.: "Delayed Catabolism of high density lipoprotein apoprotein A-I and A-II in human cholesterol ester transfer protein deficiency." J.Clin.Invest.92. 1650-1658 (1993)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi