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Anabolic Effects of Vitamin D on Bone Remodeling : Correlation between Angiegenesis and Osteogenesis

Research Project

Project/Area Number 09671077
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionTokyo Women's Medical University

Principal Investigator

SATO Kanji  Institute of Clinical Endocrinology, Tokyo Women's Medical University, Associate Professor, 医学部, 助教授 (60138857)

Co-Investigator(Kenkyū-buntansha) WANG Da-Shen  Institute of Clinical Endocrinology, Tokyo Women's Medical University, Research
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsVascular endothelial growth factor (VEGF) / Osteoblasts / Vascular endothelial Cells / human Growth Hormone (hGH) / Insulin-like growth factor-1 (IGF-1) / Endothelin-1 / VEGF receptors / active vitamin D / 血管内皮細胞増殖刺激因子 / オステオカルシン / interleukin-6(IL-6) / アルカリ・フォスファターゼ / Vascular endothelial growtn facter VEGF
Research Abstract

Human osteoblast-like cells (HOB) produces constitutionally vascular endothelial growth factor (VEGF) mRNA, and its steady state expression level is increased by active vitamin D (1, 25-(OH)2D3), (Wang DS, et al. J Bone Miner Res 11 : 472-479, 1996). It is well known that bone formation develops around blood vessels. 1, 25-(OH)2D3), which is most widely used for patients with osteoporosis, is capable of increasing (or at least maintaining) bone mineral density particularly in patients with senile osteoporosis.
We therefore investigated weather 1, 25-(OH)2D3 is capable of stimulating proliferation and differentiation of HOB, when cocultured with human umbilical vein endothelial cells (HUVEC). 1, 25-(OH)2D3 did not stimulate proliferation of HOB alone, however, when HOB were cocultured with HUBEC, 1, 25-(OH)2D3 stimulated proliferation and differentiation of HOB.This anabolic effects were mediated by VEGF produced by HOB and insulin-like growth factor type I (IGF-1) and endothelin-1 produced by HUVEC.RT-PCR revealed that VEGF produced by HOB stimulated expression of VEGF receptors type I (FLT) and type II (KDR) in HUVEC (Wang DS, et al. Endocrinology 138 : 2953-2962, 1997).
Furthermore, we have demonstrated that human growth hormone (hGH) elicits anabolic effects on HOB, by stimulating production of osteocalcin. HGH may stimulate bone remodeling by stimulating production of interleukin-6 (Wang DS, et al. Endocrine J.46 : 125-132, 1999). Weather hGH stimulates VEGF and IGF-1 in HOB and elicits anabolic effects on HOB is under the present investigation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Wang DS, Miura M Demura H Sato K.: "Anabolic effcots of 1.25-clihydroxyvitam in D_3 on osteoblasts are enhanesd by vascular endothelial growth factor produced by -" Endocrinology. 138. 2953-2962 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang D.S, Sato K, Demura H.Kato Y Miyacho Y: "Osteo-anabilie effects of human growth hormone cvoth 22K-and 20K daltons or human ostcoblaet lela cells" Endocrine Journal. 46. 125-132 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang DS,Yamazaki K,Nohtomi K,Shizume K,Ohsumi K,Shibuya M,Demura H,Sato K.: J Bone Miner Res. 11. 472-479 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang DS,Miura M,Demura H,Sato K.: "Anabolic effects of 1,25-dihydroxyvitamin D_3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells." Endocrinology. 138. 2953-2962 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang DS,Sato K,Demura H,Kato Y,Maruo N,Miyachi Y: "Osteo-anabolic effects of human Growth Hormone with 22K-and 20K Daltons on Human Osteoblast-like Cells." Endocrine J. 46(1). 125-132 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang DS,Miura M,Demura H,Sato K: "Anabolic effects of 1.25-dihydroxy vitamin D_3 on ostcoblaots are enhanced by vascular endothelial growth factor produced by -" Endocrinology. 138. 2953-2962 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wang DS,Sato K,Demura H,Kato Y,Miyachi Y.: "Ostco-anobolte effects of human growth hormone with 22k- and 20k deltone on human ostublert-bke cel" Gnldocsine Journal. 46. 125-132 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] WangDs, Miuram, Demura H.Sato K: "Anabolic effects of 1,25-dihydroxyvitimin D_3 on osteo Hactsare." Endocrinolohy. 138. 2953-2962 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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