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Mcchanisms of homing for hematopoietic progenitor cells after bone marrow transplantation

Research Project

Project/Area Number 09671114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionOkayama University

Principal Investigator

SHINAGAWA Katsuji  Okayama University, Medical School Hospital, Assistant, 医学部附属病院, 助手 (00273988)

Co-Investigator(Kenkyū-buntansha) TAKENAKA Katsuto  Okayama University, Medical School Hospital, Assistant, 医学部附属病院, 助手 (30301295)
ISHIMARU Fumihiko  Okayama University, Medical School Hospital, Assistant, 医学部附属病院, 助手 (50284097)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsprogenitor cells / transplantation / cell transplantation / homing / donor / colony-forming unit / C57BL mouse / ホーミング / Colony Farming Unit / ホ-ミング / コロニー / 接着因子 / 骨髄内皮細胞
Research Abstract

We investigated the homing mechanisms for hematopoictic progenitor cells after bone marrow transplantation (BMT). (1)We analyzed the number of myeloid proginitor cells (CFU-GM) of periplicral blood (PB) on day 1 to 35 after allogeneic-BMT and high proliferative potential colony-forming-cells (HPP-CFC) of the harvested donor BM and day 1 PB of recipients. The number of CPU-GM decreased and became undetectable on day 5 and reappeared on day14. Thc proportion of 1-IPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM and thc donor origin of these HPP-CFC were confirmed by VNTR analysis. More than 90% of these HPP-CFC had replating potential revealed by secondary colony aasay. And the proportion of CD34 CD38 ^<negative> cells was significantly higher in day 1 PB than in the harvested BM by two-color flow cytometric analysis. (2)We performed the same analysis in allogeneic-peripheral blood stem cell transplantation(PBSCT) that was newly establishe … More d in recent years. The number of CPJ-GM decreased and diminished on day 5 and emerged again on day 10 or 14 after allogenei-PBSCT.(3)We analyzed the posttransplant kinetics of CPU-GM in two femurs of C57BL black mice on 30 mm. to 24 hrs after BMF.The number of CFU-GN4 decreased at first and indicated the bottom on 12hrs and increased again. From this data, homing receptor blocking assay was performed. BM cells were pretreated with anti-VLA-4 antibody, anti-VLA-5 antibody, anti-LFA-1 antibody and anti-L-selectin antibody and tarnsplanted. Scedong efficiency was analyzed by the ratio of CFU-GM that seeded in two femoral BM to the number of transplanted CFU-GM at the time of 3Omin., 2brs, 24hrs. In all antibody pretreated mice, the number of CPU-GM was significantly decreased at every time, except for VLA-4 the decrease was significant only at 24 hrs. These observations suggested that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allogeneic-BMT in both human and mouse, and in BMT models of CS7BL mouse some adhesion molecules effect the homing efficiency of transplanted BM cells. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Harada M, et al: "Allogeneic peripheral blood stem cell transplantation for standard-risk leukemia. A multicenter pilot study : Japanese experience" Bone Marrow Transplant. 21(suppl 3). S54-S56 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naira M, et al: "Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell Transplantation" Int J Hematol. 69. 36-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katayama Y, et al: "Replating potential of colony-forming units of granulocytes/macrophages(CFU-GM) expanded ex vivo by stem cell factor, interleukin(IL)-3, IL-6, granulocyte colony-stimulating factor, erythropoietin with or without thrombopoietin" Int Hematol. 68. 157-168 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maeda Y, et al: "Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation" Br J Haematol. 105(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katayama Y, et al: "Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period" Bone Marrow Transplant. 23(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Harada M,et al: "Allogeneic peripheral blood stem cell transplantation for standard-risk leukemia. A multicenter pilot study : Japanese experience" Bone Marrow Transplant. 21 (suppl 3). S54-S56 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naira M,et al: "Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell transplantation" Int J Hematol. 69. 36-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katayama Y,et al: "Replating potential of colony-forming units of granulocytes/macrophages (CFU-GM) expanded ex vivo by stem cell factor, interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor, erythropoietin with or without thrombopoietin" Int J Hematol. 68. 157-168 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maeda Y,et al: "Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation" Br J Haematol. 105 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katayama Y,et al: "Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period" Bone Marrow Transplant. 23 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Harada M, et al.: "Allogeneic peripheral blood stem cell transplantation for standard-risk leukemia A multicenter pilot study : Japanese experience" Bone Marrow Transplant. 21(suppl 3). S54-S56 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naira M, et al.: "Analysis of circulating hematopoietic progenitor cells after peripheral blood stem cell Transplantation" Int J Hematol. 69. 36-42 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katayama Y, et al.: "Replating Potential of colony-forming units of granulocytes/macrophages (CFU-GM) expanded ex vivo by stem cell factor, interleukin (IL) -3, IL-6, granulocyte colony-stimulating factor, erythropoietin with or without thrombopoietin" Int J Hematol. 68. 157-168 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda Y, et al.: "Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation" Br J Haematol. 105(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katayama Y, et al.: "Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period" Bone Marrow Transplant. 23(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 原田実根: "症例に学ぶG-CSFの臨床" 医薬ジャーナル社, 212 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 池田康夫: "新しい造血幹細胞移植" 南江堂, 214 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yano T, et al.: "G-CSF-induced mobilization of peripheral blood stem cells for allografting : Comparative study of daily single versus double dose of G-CSF" Int J Hematol. 66 (2). 169-178 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Katayama, et al.: "The effects of a simplified method for cryopreservation and thawing procedures on peripheral blood stem cells" Bone Marrow Transplant. 19. 283-287 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nawa Y, et al.: "Respones of G-CSF mobilized peripheral blood mononuclear cells to alloantigen stimulation" Blood. 90. 1716-1718 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Teshima T, et al: "Circulating immature cell counts on the harvest day predict the yields of CD34+ cells collected after G-CSF plus chemotherapy induced mobilization of peripheral blood stem cells" Blood. 89. 4660-4661 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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