| Project/Area Number |
09671129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KAJII Eiji Jichi Medical School, Dept.of Medicine, Professor, 医学部, 教授 (40204391)
|
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| Co-Investigator(Kenkyū-buntansha) |
OMI Toshinori Jichi Medical School, Dept.of Medicine, Lecturer, 医学部, 助手 (40296091)
OKUDA Hiroshi Jichi Medical School, Dept.of Medicine, Lecturer, 医学部, 助手 (50285772)
IWAMOTO Sadahiko Jichi Medical School, Dept.of Medicine, Assistant Prof., 医学部, 助教授 (10232711)
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| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | autoimmune hemolytic anemia / Rh polypeptide / Rh system / Rh antigen / autoantigen / epitope / autoantibody / cDNA |
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| Research Abstract |
In autoimmune hemolytic anemia (AIHA), the determination of the epitopes of autoantigens directed with anti-red blood cells (RBC) autoantibodies is very important for revealing the mechanism in the failure of immunotolerance. We planed analysing the specificities of autoantibodies eluted from RBC of 21 AIHA patients. For the purpose of preparing panel cells, we constructed retroviral gene coding 4 Rh-cDNAs, which contain RhD, cE, ce, and CE, and introduced them into KU812E cells. All the Rh antigens other than RhC were confirmed to be expressed by flow cytometory. The reactivities of autoantihodies against these panel cells were examined by flow cytometory. The results showed specificities against the Rh polypeptides in 10 of 21 cases (47.6%). Nine of these 10 autoantibodies reacted with the RhC(c)E(e) polypeputide and three did with the RhD polypeptide. These data suggested that the RhC(c)E(e) polypeptide play an important role in pathogenesis of AIHA and that the epitopes directed with autoantibodies exist on the common region of the Rh polypeptides.
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