| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
Expression of I and sialosyl-l antigens was examined using specific monoclonal antibodies. The anti-I antibody reacted with monocytes, T cells, B cells but not with neutrophils, bone marrow (BM) CD34^+ cells or mobilized peripheral blood (PB) CD34^+ cells. The anti-sialosyl-l antibody reacted with monocytes and BM CD34^+ cells but not with T cells, B cells or neutrophils and it hardly reacted with mobilized PB CD34^+ cells. Macrophages differentiated from monocytes showed increased expression of I and decreased expression of sialosyl-l. BM CD34^+ cells cultured in medium containing cytokines produced l^+ monoblasts and sialosyl-l^+CD34^+ monoblasts. Flow cytometric analysis of CD34^+ cells enriched from BM showed that sialosyl-l^+ cells expressed CD13, CD33, CD117, and HLA-DR.Sialosyl-l^+CD34^+ cells isolated from BM mainly produced granulocye-macrophage colonies and pure macrophage colonies. Therefore, sialosyl-l^+CD34^+ cells are suggested to be colony-forming units granu locyte-macrophage (CFU-GM). Our data indicate that : 1) the sialosyl-l antigen is selectively expressed along the differentiation of CFU-GM to monocytes, and 2) the I antigen is expressed at later stages of the monocytic differentiation, and 3) the I antigen is broadly expressed in monoblasts, monocytes, macrophages, and lymphocytes.
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