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Analysis of lymphocyte maturation after purified hematopoietic stem cell transplantation and induction of tumor immunity.

Research Project

Project/Area Number 09671145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionAICHI CANCER CENTER

Principal Investigator

MORISHIMA Yasuo  RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (20220056)

Co-Investigator(Kenkyū-buntansha) KOGAMI Yoshitoyo  RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (30270721)
OGURA Michinori  RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (40231229)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordshematopoietic stem cell transplantation / CD34 positive stem cell. / tumor immunity / maturation of lymphocyte. / EB virus. / NK / T cell lymphoma / cell line
Research Abstract

Immunologocal recovery after purified hematopoietic stem cell transplantation was analyzed in 15 patients with hematological malignancy. 2.3x 10\o\ac (\s\up 90,7)/kg (median) of CD34 positive hematopoietic stem cells were purified by Isolex System using anti-CD34 monoclonal antibody and immuno-magnetic beads. Lymphocyte/NK cell subpopulations in peripheral blood were monitored after transplantation. Increase of CD57+, DC8+ cell was observed after one month of transplantation. One patient transplanted by preconditioning of total body irradiation (TBI) and melphalan showed a remarkable increase of CD57+, CD8+ cells after cytomegarovirus infection, and its increse was persited for more than 8 months with abnormarity of chromosome in 2/20 PB cells and oligoclonal TCR rearrangement bands. Including this patient, high frequency of opportunistic infections was observed in patients treated with TBI and CD34 positive selection. These findings suggest that add-back of T cells is necessary in order to reconstitute the immunological deficient state after CD34 positively selected transplantation
In order to make the model of adoptive immunotherapy for malignangt lymphoma, we established a IL-2 dependent culture cell line from nasal type NK/T cell lymphoma. This cell line was CD2+, sCD3-, CD3ε+, CD8-, CD16+, CD56+, CD94+, CD158a, b-and TIA1+, granzyme B+. Clonal integration of EB virus was detected, and type II latency pattern (LMP-1+, EBNA2-) of EB virus infection was observed. This cell line will be a useful target cell for the induction of cytotoxic T cells against EB virus cell suface antigen.

Report

(3 results)
  • 2000 Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Michinon Ogura A Yasuo Morishima et al: "Phase [/[[ trial of cure-orienced high-dose chemoradiotherapy with transplantation of CD34+peripheral blood stem cells"Cancer Chemother Pharmacol. 40. 51-57 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshitoyo Kagami Yasuo Morshima et al: "Establishment of sn IL-2-domendent cell lines derived from nasal type NK/T lymphoma"British Journal of Haematology. 103. 669-677 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Horoshi Sao Yasuo Morishima et al: "A new T cell depletion method and its application for prevention of acute GVHD in allogeneic BMT"International Journal of Hematology. 69. 27-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Michinori Ogura, Yasuo Morishima et al.: "Phase I/II trial of cure-oriented high-dose chemoradio-therapy with transplantation of CD34+ peripheral blood stem cells"Cancer Chemother Pharmacol. 40. 51-57 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihisa Kagami, Yasuo Morishima et al.: "Establishiment of an IL-2 dependent cell lines derived from nasal type NK/T lymphoma."British J Haematol. 102. 669-677 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hiroshi Sao. Yasuo Morishima et al.: "A new T cell depletion method and its application for prevention of acute GVHD in allogeneic BMT."Intern J Hematol. 69. 27-35 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Michinori Ogura Yasuo Morishima et al: "Phase I/II trial of cure-oriented high-dose chemoradiotherapy with transplantation of CD34+ peripheral bloodstem cells." Cancer Chemother Pharmacol. 40. 51-57 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshitoyo Kagami Yasuo Morishima et al: "Establishment of an IL-2-dependent cell lines derived from nasal type NK/T llymphoma." Britishi Journal of Haematology. 103. 669-677 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Horoshi Sao Yasuo Morishima et al: "A new T cell depletion method and its application for prevention of acute GVHD in allogeneic BMT" International Journal of Hematology. 69. 27-35 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Michinori Ogura: "Phase I/II trial of cure-oriented high-dose chemoradiotherapy with transplantation of CD34+ peripheral blood stem cells." Cancer Chemother Pharmacol. 40(Suppl). 51-57 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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