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Study of the relationship between molecular structure and function in epithelial sodium channel.

Research Project

Project/Area Number 09671173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionJichi Medical School

Principal Investigator

ONO Shuichi  Jichi Medical School, Dept.Nephrol., Instructor, 医学部, 助手 (90285776)

Co-Investigator(Kenkyū-buntansha) MUTO Sigeaki  Jichi Medical School, Dept.Nephrol., Assistant Prof., 医学部, 講師 (40190855)
KUSANO Eiji  Jichi Medical School, Dept.Nephrol., Associate Prof., 医学部, 助教授 (50102249)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsinner medulla / inner medullary collecting duct / rat epithelial Na^+ channel / low-Na^+ diet / rENaC / Na^+ transport / Inner medulla / Inner medullary collecting duct / Low-Na^+ diet / Rat opithelial Na^+channel / Na^+transport
Research Abstract

The' purpose of the present study was to determine whether the renal inner medulla expresses mRNA for the rat epithelial Na^+ channel (rENaC), and, if so, to define their regulatory properties using a low Na^+ diet model. We have developed a specific probe for alpha subunit using RT-PCR with rENaC c subunit specific primers. Probe for rENaC ct subunit hybridized not only to distal colon RNA but also to inner medulla RNA derived from normal diet rats. we examined the effect of low Na^+ diet on alpha, beta and gamma subunits mRNA expression of rENaC using full length cDNA as a probe. A marked elevation of rENaC ct subunit mRNA abundance in inner medulla was observed in response to high aldosterone plasma concentration induced by dietary Na^+ deprivation. On the other hand, neither beta nor gamma subunits mRNA expression was enhanced by low Na^+ diet. From these results, it is suggested that rENaC is responsible for Na^+ transport in renal inner medulla and is probably regulated via transcriptional control of the alpha subunit of ENaC.
Furthermore, we are now raising peptide-directed polyclonal antibodies to the a, beta and gamma subunits of ENaC.Immunofluorescence labeling with beta or gamma antibodies revealed apical localization in some distal tuble cells from rat kidney. Further studies will be required to evaluate the specificity of antibodies for beta or gamma subunits of ENaC.The relationship between molecular structure and function will be studied.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Shuichi Ono et.al.: "A low-Na^+ diet enhances expression of, mRNA for epithelial Na^+ channel in rat renal inner medulla." Pflugers Arch-Eur J Physiol. 434. 756-763 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 大野 修一, 他: "Liddle's syndrome 類似の病態をもつ多発性嚢胞腎患者の上皮性ナトリウムチャネル遺伝子解析" Therapeutic Research. 19. 181-186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono, S., E.Kusano, S.Muto, Y.Ando and Y.Asano.: "A low-Na^+ diet enhances expression of mRNA for epithelial Na^+ channel in rat renal inner medulla." Pflugers Arch-Eur J Physiol. 434. 756-763 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono, S., E.Kusano, Y.Ando, S.Muto, S.Sato, S.Asakura and Y.Asano.: "Genetic analysis of epithelial Na+ channel (ENaC) in a case of polycystic kidney disease patient, who had Liddle's syndrome like symptoms." Ther Res. 19. 181-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono, S., E.Kusano, S.Muto, Y.Ando and Y.Asano.: "mRNA expression for rENaC subunits is differentially regulated by low Na diet in rat renal inner medulla." J Am Soc Nephrol. 8. 41A (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 大野修一、他: "Liddle's Syndrome類似の病態をもつ多発性嚢胞患者の上皮性ナトリウムチャネル遺伝子解析" Therapeutic Research. 19. 181-186 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shuichi Ono: "A low-Na^+ diet enhances expression of mRNA for epithelial Na^+ channel in rat renal inner medulla." Pflugers Arch-Eur J Physiol. 434. 756-763 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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