Nitric oxide and neonatal hypoxic-ischemic encephalopathy
| Project/Area Number |
09671191
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Embryonic/Neonatal medicine
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
HATTORI Haruo Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (80189571)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | hypoxic-ischemic encephalopathy / neonate / nitric oxide / neuronal nitric oxide synthase / inducible nitric oxide synthase |
|---|
| Research Abstract |
The neuroprotective effect of a selective inducible nitric oxide synthase (iNOS) inhibitor ; aminoguanidine, was evaluated in a neonatal hypoxic-ischemic rat model. We also measured the changes in nitric oxide (NO) metabolites in the brain. Unilateral hypoxic-ischemic injury was produced in the brain of 7-day-old rats by combining a common carotid artery ligation with hypoxic exposure (8% oxygen) for 2.5h. Histological evaluation of the brain damage was performed 72 h later. Brain NO metabolites were measured by using a NO analyzer at 0 h, 1.5 h (during hypoxia), 2.5 h (end of hypoxia), 6 h, 24 h, and 72 h after the start of hypoxia. Aminoguanidine (300 mg/kg) was injected intraperitoneally nine times at eight-hour intervals starting 1 h before the hypoxia. These selective iNOS inhibitor administrations, resulted in 89% reduction of both the cerebral cortical and the striatal infarcted areas compared to those of vehicle injected controls. Repeated injections of aminoguanidine also suppressed the peak of NO metabolites in the brain during the reoxygenation period. These results indicate that NO generated by iNOS during reoxygenation plays a key role in producing neonatal hypoxic-ischemic brain damage.
|
Report
(3 results)
Research Products
(8 results)
