| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
Experiment 1> Orthotopic LTx usingpigs were performed. In the donors, cardiac arrest was induced by KCl injection and the liverwas subjected to 90 min of warm isehemia. Thereafter, the liverwas harvested and stored for 4 lx)urs usingcold Euio-Collins solution In the treated group, TAK-044 was administered to both donons and recipients at the dose of 10 mg/kg All the untreated recipient pigs died within 12 hr after operation, whereas all of the treated pigs surviwd more than 4 days (p<0.01). The improved survival seen in the treated group was associated with a reduction in the serum concentrations of GOT and LDH, a restoration of hepatic energy charge, and a suppression in the portal venous pressure. Concentrations of GOT and LDH in the washed out preservation solution at the time of graft rinsing immediately before RPF, were cIosely correlated with the graft outcome, and were significantly lowerin the treated group. <Experiment 2> In the domrs, cardiac arrest was induced by stopping the ventilator. The liver was subjected to 45 min of warm is chemia followed by 8 hr cold preservation using the UW solution TAK-044 was administered only to recipients at the dose of 10 mg/kg Postoperative survival as well as graft function were significantly better in treated group than in the untreated group. Conclusions. The present study suggests that TAK-044 can improve the hepatic allograft function, indicatinga potential for use in cIinical LTx from NHBD.The concentrations of GOT and LDH in the washed out preservation solution may provide an accurate means of evaluating liver allograft viability before LTx
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