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The mutation enhancement in human cell lines by serum factors from pancreatic cancer patients

Research Project

Project/Area Number 09671209
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionChiba University

Principal Investigator

YAMAMORI Hideo  Chiba Univ.Med.Surgery Lecturer, 医学部, 講師 (00166836)

Co-Investigator(Kenkyū-buntansha) TASHIRO Tsuguhiko  Chiba Univ.Med.Surgery, Associate Professor, 医学部, 助教授 (70143310)
SUZUKI Nobuo  Chiba Univ.Med.Biochemi., Professor, 医学部, 教授 (90111426)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordspancreatic cancer / serum factor / K-ras / mutation / protease / 膵臓 / 癌遺伝子
Research Abstract

In pancreatic cancer cells base institution mutation is very highly detected. We have reported that sera from pancreatic cancer patients have the ability to enhance the mutation frequency. In the present research effects of the sera on protein and DNA metabolism were studied, in order to clarify mechanism of the enhancement activity. We have already found that human interferon induce the activity of antipain-sensitive proteases in early steps of suppression of the mutation incidence. Thus, fibrinolysis activity was estimated using ^<125>I-fibrin as substrates in cells pretreated with serum factors from pancreatic cancer patients and then irradiated with UV.The factors were obtained by a color-column chromatography method. The fraction sample was obtained which enhance frequencies of ouabain-resistant phenotypic mutation induced by UV.The fraction sample also showed the ability to enhance frequencies of K-ras codon 12 mutation detected by the PCR-based differential dot-blot hybridization analysis. Furthermore the sample enhance the proteases activity induced by UV On the other hand, induction of several genes expression was identified by the RT mRNA differential display method.
Therefore, it was suggested that serum factors from pancreatic cancer patients enhance K-ras codon 12 mutation possibly via proteases induction followed by several genes expression.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Suzuki, N., suzuki, H., Ishizuka, T., Yamamori, H.and Ino, H.: "UV-induced mutations affecting codon12 of the K-ras gene are suppressed by interferon-α in human Rsa cells." Mutation Research. 373. 251-256 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Isogai, E, Ishijima, S., Sonoda, T., kita, K., Suzuki, H., Haseagwa, R., Yamamori, H., Takakubo, Y., and Suzuki, N.: "Protease activation following UV irradiation is linked to hypomutability in human cells selected for resistance to combination of UV and antipain" Mutation Res. 403. 215-222 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugita, K, Suzuki, N., Higuchi, Y., Kita, K., Suzuki, Y., and Lehmann, A: "Enhancement of XP-G mRNA expression by human interferon-β in Cockayne syndrome cells" Mutation Res. 408. 67-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, N, Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S. and Suzuki, N.: "Mutagenicity of microcystin-LR in human RSa cells" International Journal of Molecular Medicine. 2. 109-112 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Higuchi, Y, Kita, K., Nakanishi, H., Wang, XL., Sugaya, S., Yanzawa, H., Yamamori, H., Sugita, K., Yamaura A. and Suzuki, N.: "Search for genes involved in UV-resistance in human cells by mRNA differential displey : increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance" BBRC. 248. 597-602 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, N., Ishibashi, M., Kita, K., Wu, Y., Nomura, J., Takakubo, Y., Hiroshima, K., Genga, K., Ohwada, H. and Hayashi, Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells" Int.J.Cancer. 78. 550-555 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wu, Y., Hiwasa, T, Isogai, E., Sonoda, T., Kita, K., Chen, Z., Sugaya, S., Yamamori, H., Takazawa, H. and suzuki, N.: "Activation of MAP Kinases by 5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line" Int.J.Oncol.13. 1241-1245 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kikuno, K., Kita, K., Nomura, J., Hiwasa, T., Yonemitsu, H. and Suzuki, N.: "Search for genesresponsible for UV susceptibility of human cells : involvment of syndecan-1 in UV resistance" Biochem. Biophys. Res. Commun.253. 519-523 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuguhiko Tashiro, Hideo Yamamori, Kazuya Takagi, Naganori Hayashi, Toshiyuki Sugiura, and Nobuyuki Nakajima: "The effect of newly deveroped fat emulsion containing eicosapentaenoic acid and docosahexaenoic acid on fatty acid profiles in rats" Nutrition. 14. 372-375 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuguhiko Tashiro, H.Yamamori, N.Hayashi, K.Furukawa, Y.Morishima, T.Sugiura, T.Itavashi, W.sano, Y.Toyoda, H.Nitta, J.Hirano, K.Nishiya and N.Nakajima, M.D.: "N-3 versus N-6 polyunsaturated fatty ascids (PUFA) in critical illness" Nutrition. 14. 551-553 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Hayashi, T.Tashiro, H.Yamamori, K.Furukawa, Y.Morishima, T.Sugiura, T.Itabashi, W.Sano, Y.Toyoda, H.Nitta, J.Hirano, K.Nishiya and N.Nakajima: "Effect of intravenous n-3 and n-6 fat emulsion and delayed type hypersensitivity in burned rats receiving total parenteral nutrition" J Parent Ent Nutr. 22. 363-367 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fuji, K., Suzuki, N., Kita, K., Ishijima, S,Sonoda, T., Dezawa, M., Sugita, K.and Niimi, H.: "Abnormal DNA synthesis activity induced by X-rays in nevoid basal cell carcinoma syndrome cells." Biochem.Biophys.Res.Commun.240. 269-272 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, N., Suzuki, H., Ishizuka, T., Yamamori, H.and Ino, H.: "UV-induced mutations affecting codon 12 of the K-ras gene are suppressed by interferon-alpha in human Rsa cells." Mutation Research. 373. 251-256 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Isogai, E,Ishijima, S., Sonoda, T., Kita, K., Suzuki, H., Hasegawa, R., Yamamori, H., Takakubo, Y., and Suzuki, N.: "Protease activation following UV irradiation is linked to hypomutability in human cells selected for resistance to combination of UV and antipain" Mutation Res.403. 215-222 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugita, K,Suzuki, N., Higuchi, Y., Kita, K., Suzuki, Y., and Lehmann, A.: "Enhancement of XP-G mRNA expression by human interferon-beta in Cockayne syndrome cells" Mutation Res.408. 67-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, H,Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S.and Suzuki, N.: "Mutagenicity of microcystin-LR in human RSa cells." International Journal of Molecular Medicine. 2. 109-112 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Higuchi, Y,Kita, K., Nakanishi, H., Wang, XL., Sugaya, S., Tanzawa, H., Yamamori, H., Sugita, K., Yamaura A.and Suzuki, N.: "Search for genes involved in UV-resistance in human cells by mRNA differential display : incrased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance." BBRC. 248. 597-602 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, N., Ishibashi, M., Kita, K., Wu, Y., Nomura, J., Takakubo, Y., Hiroshima, K., Genga, K., Ohwada, H.and Hayashi, Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells." Int.J.Cancer. 78. 550-555 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wu, Y., Hiwasa, T,Isogai, E., Sonoda, T., Kita, K., Chen, Z., Sugaya, S., Yamamori, H., Takazawa, H.and Suzuki, N: "Activation of MAP Kinases by-5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line." Int.J.Oncol.13. 1241-1245 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kikuno, K., Kita, K., Nomura, J., Hiwasa, T., Yonemitsu, H.and Suzuki, H.: "Search for genes responsible for UV susceptibility of human cells : involvment of syndecan-1 in UV resistance." Biochem.Biophys.Res.Commun.253. 519-523 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Isogai,E,Ishijima,S.,Sonoda,T.,Kita,K.,Suzuki,H.,Hasegawa,R.,Yamamori,H.,Takakubo,Y.,and Suzuki,N.: "Protease activation following UV and inradiation is linked to hypomutability in human cells selected for resistance to combiuatiou of UV antipain." Mutation Res.403. 215-222 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugita,K,Suzuki,N.,Higuchi,Y.,Kita,K.,Suzuki,Y.,and Lehmann A.: "Enhancement of XP-G mRNA expression by human interferon-β in Cockayne syndrome cells." Mutation Res.408. 67-72 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki,H,Watanabe,M.,Wu,Y.,Sugita,T.,Kita,K.,Sato,T.,Wang,X.,Tanzawa,H.,Sekiya,S.and Suzuki,N.: "Mutagenicity of microcystin-LR in human RSa cells." International Journal of Molecular Medicine. 2. 109-112 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Higuchi,Y,Kita,K.,Nakanishi,H.,Wang,XL.,Sugaya,S.,Tanzawa,H.,Yamamori,H.,Sugita,K.Yamaura A.and Suzuki,N.: "Search for genes involved in UV-resistance in human cells by mRNA differential display:increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance." BBRC. 248. 597-602 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki,N.,Ishibashi,M.,Kita,K.,Wu,Y.,Nomura,J.,Takakukbo,Y.,Hiroshima,K.,Genga,K.,Ohwada,H.and Hayashi,Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells." Int.J.Cancer. 78. 550-555 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wu,Y.,Hiwasa,T,Isogai,E.,Sonoda,T.,Kita,K.,Chen,Z.,Sugaya,S.,Yamamori,H.,Takazawa,H.and Suzuki,N.: "Activation of MAP kinases by 5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line." Int.J.Oncol.13. 1241-1245 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kikuno,K.,Kita,K.,Nomura,J.,Hiwasa,T.,Yonemitsu,H.and Suzuki,N.: "Search for genes responsible for UV susceptibility of human cells:involvment of syndecan-1 in UV resistance." Biochem.Biophys.Res.Commun.253. 519-523 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuguhiko Tashiro,Hideo Yamamori,Kazuya Takagi,Naganori Hayashi,Toshiyuki Sugiura,and Nobuyuki Nakajima.: "The effect of newly devel oped fat emulsion containing eicosapentaenoic acid and docosahexaenoic acid on fatty acid profiles in rats." Nutrition. 14. 372-375 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuguhiko Tashiro,H.Yamamori,N.Hayashi,K.Furukawa,Y.Morishima,T.Sugiura,T.Itabashi,W.Sano,Y.Toyoda,H.Nitta,J.Hirano,K.Nishiya and N.Nakajima,M.D.: "N-3 versus N-6 polyunsaturated fatty ascids(PUFA) in critical illness." Nutrition. 14. 551-553 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hayashi,T.Tashiro,H.Yamamori,K.Furukawa,Y.Morishima,T.Sugiura,T.Itabashi,W.Sano,Y.Toyoda,H.Nitta,J.Hirano,K.Nishiya and N.Nakajima.: "Effect of intravenous n-3 and n-6 fat emulsion and delayed type hypersensitivity in burned rats receiving total parenteral nutrition." J Parent Ent Nutr.22. 363-367 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Tashiro,Ishiodori K,H.Yamamori,Takagi K,Nakajima N.: "Glucose oxidation in critically ill patients measured with stable isotope and indirect calorimetry" Chiba Med J.74. 393-399 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki N, Suzuki H, Ishizuka T, Yamamori H et al.: "UV-induced mutations attecting ccdon 12 of the K-ras gene gene suppressed by interteron-α in human RSa cells" Mutation Res. 373. 251-256 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimoda N, Tashiro T, Yamamori H, et a: "Effects of growth hormone and insulin-like growth factor-1 on protein metabclism,gut morpnology and cell medieted immunity in hurneal rats" Nutrition. 13. 540-546 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Abe M, Kawano M, Tashiro T, Yamamori Het al.: "Catabolism of lipoprotein X inducedby infusion of 10% fat emulsion" Nutrition. 13. 417-421 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sugiura T, Tashiro T, Yamamori H et al: "Effects of insulin-like growth factor 1 on endotoxin translocation in burned rats receiving total parentoerel nutrition" Nutrition. 13. 783-787 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 林求規、田代亜彦、山森秀夫他: "侵襲下におけるnー涼脂肪乳剤の投与量が免疫反応に与える効果とGranlucyte-macropnage colony stimulating factorの関与" 外科と代謝・栄養. 31. 225-232 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木信夫、山森秀夫他: "ストレス感受性突然変異促進因子" Bio Review. 14. 5-9 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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