|Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
The effects of n-6 and n-3 PUFA on protein metabolism, cell-mediated immunity, and production of cytokines and prostanoids were studied.
Experiment 1 : Three groups of rats were fed by TPN including safflower oil emulsion (20% of total energy ; group"N6"), 19% of safflower oil and 1% of structured EPA and DHA (2 : 1)("N3") and without fat ("Control"), and were subjected to 20% full-thickness scald burns. "N6"demonstrated increased serum IL-6 and TNFa (p<0.05), and decreased nitrogen balance (NB), when compared with "Control". But in "N3", TNF and IL-10 were reduced, and NB was improved. Suppressed delayed type hypersensitivity (DTH) induced by burn injury, was significantly improved in "N3", when compared with "Control" and "N6".
Experiment 2 : Forty-three rats were fed by TPN including safflower oil (%)/fish oil (%) fat emulsion (0/0, 0/20, 4116, 10/10 and 20/0). DTH, GM-CSF and EPA increased, and gut endotoxin translocation decreased proportionately with the intravenous dose of fish oi
l emulsion. GM-CSF correlated significantly with DTH.
Experiment 3 : Six esophagectomized patients received fat-free TPN ("E-gl"), and 6 received TPN with Intralipid (20% of total energy, "E-fat"). EPA methyl ester (1.8g/day) were administered orally or enterally to 7 who received TPN with Intralipid ("E-EPA"). In group "E-fat", serum IL-6 significantly increased at 2 and 6 hours after operation when compared with "E-gl". In "E-EPA", postoperative IL-6 was reduced and cell-mediated immune function was improved significantly.
Experiment 4 : In 5 esophagectomized patients fed enterally with EPA methyl ester, ConA and PHA stimulated lymphocyte proliferation, NK activity and CD4/8 were improved significantly during the chemo-radiation therapy when compared with the patients without EPA (n= 14).
Conclusion : N-6 PUFA amplified, and n-3 PUFA reduced the stress response and immunosuppression induced by burn injury or surgical stress. N-3 PUFA is potentially beneficial for the critically ill patients. Less