| Project/Area Number |
09671221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
TAKAO Hiroshi SCHOOL OF MEDICINE GIFU UNIVERSITY CANDIDATE, 医学部・附属病院, 講師 (50144035)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | drug delivery system / IL-2 / OK-432 / w / o emulsion / o / w emulsion / cマウス / colon26 / 細胞傷害活性 / NK活性 / W / Oエマルション / O / Wエマルション / 細胞障害活性 / O型エマルション / W型エマルション / MRMT-1 / o型エマルジョン / w型エマルジョン / Drug delivery system / O型エマルジョン / W型エマルジョン / アポトーシス / colon 26 |
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| Research Abstract |
【purpose】 We developed who type and w/o/w type emulsions as a drug delivery system. The emulsions which encapsulate BRM agents, were administrated for locoresional immunotherapies against cancer. Some trials for improvement of the emulsion was made for the anticancer effects.
【materials】 SD rats with liver metastases of MRMT-1 tumor cells and BALB/c mice with liver metastases of colon26 tumor cells, both tumor cells were inoculated into the portal vein of each animals. They were the subject of this study.
【methods】 Intrasplenic administration of the emulsion encapsulating BRM (IL-2, OK-432) immediately after inoculation of tumor cells was carried out. After several days liver cells and splenic cells were extracted measured cytotoxicity against both the autologous tumor cells (MRMT-1 and colon26) or NK sensitive cells (YAC-1)
【results】 The effects of w/o/w emulsion with BRM agents was better on cytotoxicity examination in the autologous tumor cells and in NK sensitive cells than those of w/o emulsion and the solution without BRM agents. And the effect was demonstrated immunopathologically. W/o/w emulsion was proved less side effects than that of w/o emulsion by additional experiments.
【conclusion】 From the results, w/o/w emulsion encapsulating BRM is suggested as a more effective and useful drug form especially for locoresional immunotherapy.
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