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Examination of orgen - specific Immunocompetent cells by The analysis of chemokine & cytokine

Research Project

Project/Area Number 09671227
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

NAKATA Seizoh  Osaka University Hospital, Associate Professor, 医学部・附属病院, 助教授 (50116068)

Co-Investigator(Kenkyū-buntansha) ITO Toshinori  Osaka University Medical School, Associate Professor, 医学部, 助教授 (20231152)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsrat pancreas transplantation / insulin dependent diabetes mellitus(IDDM) / (amte)rejection / graftrecurrence of IDDM / Th1 / Th2 balance / guantitatiue RT-PCR / cytokine / chemokine / ウット膵移植 / Th1 / Th2バランス / 定量RT-PCR / インスリン依存型糖尿病(IDDM)
Research Abstract

We previously domonstrated that IDDM graft recurrence could be prevented with the administration of anti-ICAM-1/LFA-1 mAbs, resulting in the appearance of macrochimerism (34.3%) of donor-derived RT6^+ T cells (120 days postTx) in a Wistar-Furth (WF ; RT1^u, RT6.2) to spontaneously diabetes prone (DP ; RT1^u, RT6.1 gene carrier)-BB whole pancreaticoduodenal transplantation (PDTx) model.As NKR-P1^+ TCRalphabeta^+ (NKT) cells have recently been demonstrated to be involved in immunoregulatory functions such as the control of occurrence of autoimmune diseases, the purpose of this study was to clarify the relationship between NKT cells and IDDM recurrence in this model.In addition to the examination of cellular immune responses, the cells of the spleen and liver of these non-recurrent DP rats were analyzed in terms of NKT cells by flow cytometric analysis, and serum cytokine levels (IL-4 & IFN-gamma were determined by ELISA, as comparing with those of recurrent PDTx-DP recipients (without mA … More bs), recurrernt pancreas-alone transplanted DP (PATx-DP with mAbs), DP, and WF rats.In the spleen cells of the DP recipients that had accepted pancreatic grafts, 45.6 (35-55)% of the total splenic T cells were donor-derived RT6.2^+ T cells as early as 60 days postgrafting.A three color flow cytometric analysis showed that NKT cells had proliferated markedly, with the proportion of 20-30% in the total splenic T cells, accompanied by the increased population (absolute number) of RT6^+ NKT cells, though those of RT6^- NKT cells were similar in each group.The proliferation of donor-derived RT6.2^+ NKT cells wsa also observed in the liver of the non-recurrent DP recipients.On the other hand, macrochimerism (and the proliferation of NKT cells) was not detected in the spleen cells of the recurrent DP recipients.By ELISA, serum IFN-gamma was not detected (<13 pg/ml) in all, but IL-4 was detected as 158.8 pg/ml in the non-recurrent DP recipients.Our data indicate that donor-derived RT6^+ NKT cells which originated from the lymphoid tissues of the donor pancreaticoduodenal grafts might be related to the inhibition of IDDM recurrence with a Th2 deviation. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] A.Ito et al.: "Donar Specifie tolerance by perioperative intrathymic injection of bone marrow ulls in rat cardiac allograft model." Transplantation. 64(5). 752-757 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H-Horimoto et al.: "Transplantation tolerance by a combined therapy arith sulfatide,α-ICAM-1/LFA-1 monoclonal antibodies & FK506 in ratcandiac trausplantation." Transplant International. 11(supple I). S310-312 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Mikata et al: "Regulation of complement-mediated sntine endsthelial cell lysis by a surfau-bound form of human C4b binding protein." Transplantation. 65(3). 363-368 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori: "Model of monse pancreaticodusdenal transplantation" Microsurgery. 19. 61-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori: "The significant role of intragraft lymplroid tissnes in preventicp IDDM recurrence in whole panueation duodenal graft" Microsurgery. (in Press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ito: "Potent immunosuppressiue effects by a newly synthesized compound KFα0444" Transplant Proc. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 伊藤壽記: "箱根シンポジウム 7" 抗接着分子抗体による自然登症糖尿病BBラットに対する移植膵の免疫学的寛容誘導 中外医学社, 73-80 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Ito, T.Ito, W.Kamiike et al.: "Donor specific tolerance by perioperative intra-thymic injection of bone marrow cells in rat cardia allograft model.-The use of FK506 can shorten the necessary duration of pretransplant intrathymic condi-tioning.-" Transplantation. 64(5). 752-757 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Ito, T.Ito, W.Kamiike et al.: "Aunique mechanism of tolerance by periope-rative intrathymic injection of bone marrow cells with a short course of ALS and FK506 in rat cardiac allograft model" Transplant Proc. 29. 1137-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.Uchikoshi, T.Ito, W.Kamiike et al.: "Plancreas, but not islet transplantation, protects graft recurrence of IDDM in diabetic BB rats." Transplant Proc. 29. 753-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Horimoto, T.Ito, T.Hayashi et al.: "Transplantation tolerance by a combined therapy with sulfatide, anti-LFA-1/ICAM-1 monoclonal antibodies anf FK506 in rat cardiac transplantation." Transplant International. 11(Suppl1). S310-2 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Mikata, S.Miyagawa, K.Iwata et al.: "Regulation of complement-mediated swine endothelial cell lysis by a surface-bound form of human C4b binding protein." Transplantation. 65(3). 363-68 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Mikata, S.Miyagawa, W.Kamiike et al.: "New approach to prevent complement activation on xenograft effect of a surface-bound form of human C4b binding protein" Transplant Proc. 30. 65-66 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori, T.Ito, H.Matsuda et al.: "Model of mouse pancreaticoduodenal trans-plantation." Microsurgery. 19. 61-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Maeda, T.Ito, T.Yumiba et al.: "A composite graft of islet pus donor lymph node cells induced donor specific tolerance in a "low responder"rat combination with class IMHC disparity." Transplant Proc. 31. 749-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Maeda, T.Ito, T.Yumida et al.: "Difference of immunological responses between pancreatic and islet transplantation in a "low responder"rat combi-nation with class IMHC disparity." Transplant Proc. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ito, A.Ohkawa, T.Sawai et al.: "Potent immunosuppressive effects by a newly synthesized compound KF20444." Transplant Proc. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori, T.Ito, H.Matsuda et al.: "The significant role of intragrtaft lymphoid tissues in preventing IDDM recurrence in whole pancraticoduodenal graft." Microsurgery. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori, T.Ito, T.Yumiba et al.: "Proliferation of donor-derived NKR-P1^+TCR alphabeta^+(NKT)cells in the non-recurrent spontaneous diabetic BB rats transplanted pancreaticoduodenal grafts of Wistar Furth." Transplant Proc. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori, T.Ito, T.Yumida et al.: "IL-4 production in the IDDM non-recurrent pancreas transplanted BB rats with donor-derived NKR-P1^+TCRalphabeta^+(NKT)cells, but not in the recurrent BB rats." Transplant Proc. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Tori,T.Ito,H.Matsuda et.al.: "Model of mouse pancreaticoduoclenal transplautation" Microsurgery. 19(2). 61-65 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Tori,T.Yumiba et.al.: "IL-4 production in the IDDM non-reourrent pancreas trausplanted BB rats with donor-derived NKR P1^+TCRαβ^+ cells,but not in the reccurrent" Tranoplent proc. (in press). BB rats. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Ohkawa,T.Ito,T.Yumiba: "Immunological characteristeis of intregreft NKR-P1^+TCRαβ^+Talls in rat hepatie allegrafs" Trausplaut proc. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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