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Drug sensitivity of breast cancer according to the change of gene amplification by the addition of anti-cancer drug

Research Project

Project/Area Number 09671229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

SHIBA Eiichi  Osaka University Medical School, Associate Professor, 医学系研究科, 助教授 (90215997)

Co-Investigator(Kenkyū-buntansha) TSUKAMOTO Fumine  Osaka University Medical School, Assistant Professor, 医学系研究科, 助手 (70303964)
〓本 卓司  大阪大学, 医学部, 医員
すぎ本 卓司  大阪大学, 医学部・附属病院, 医員
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsBreast cancer / Drug Sensitivity / Anti-cancer Drug / 抗癌剤感受性 / E2 / TAM / PgR / PCR / PTHrP / MPA / Coamplification / Medroxy Progosterone-Acetate / Endocrine Therapy / Breast Cancer
Research Abstract

The level of parathyroid hormone-related protein (PTHrP) expressed in breast cancer tissue is closely related to the incidence of bone metastasis. We examined the PTHrP mRNA expression in breast cancer tissues by coamplification polymerase chain reaction (PCR) in mole ratio to internal standard beta-actin mRNA.The PTHrP expression was higher in premenopausal patients than in postmenopausal patients (P<0.05). More pronounced difference by menopause found in estrogen receptor (ER) positive groups (P<0.001) indicated that the PTHrP expression in breast cancer tissue is hormonally regulated and might be altered by endocrine agents. To clarify the changes of PTHrP expression by endocrine therapy of breast cancer, we measured PTHrP expression in the breast cancer tissue incubated for 24 h with 1×10(-8) M of estradiol (E2), 1×10(-6) M of tamoxifen (TAM) and 1×10(-5) M of medroxyprogesterone acetate (MPA). The PTHrP expression was decreased significantly by MPA (P<0.005), while E2 and TAM did not change the PTHrP expression. Progesterone receptor (PgR) mRNA expression was also examined to confirm that the breast cancer tissue responds to E2 and TAM.The results were well compatible with the better therapeutic effect of MPA reported for the treatment of breast cancer with bone metastases. As a potential candidate for the receptor that mediates the suppressive effect of MPA, androgen receptor (AR) is suggested most probable. Present results also demonstrated that the clinical response of individual tumors is closely associated with the early in vitro changes of gene expression detected in the cancer specimen.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] E.Shiba, S.Kim, J.Kambayashi, I.Kawamura, E.Lacey, T.Manda, K.Shimomura, T.Taguchi, S.Takai.: "Mechanism of growth inhibition by calpain inhibito in MCF-7 cells."Anticancer Res.. 17. 1919-1924 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] F.Tsukamoto, E.Shiba, T.Taguchi, T.Sugimoto, T.Watanabe, SJ.Kim, Y.Tanji, Y.Kimoto, M.Izukura, S.Takai.: "Immunohistochemical Detection of P-glycoprotein in Breast Cancer and its Significance as a Prognostic Factor"Breast Cancer.. 1. 259-263 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 芝英一: "乳癌のホルモン療法 補助療法におけるホルモン療法"実験治療. 646. 57-62 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Sugimoto, E.Shiba, T.Watanabe, S.Takai.: "Suppression of parathyroid hormone-related protein messenger RNA expression by medroxyprogesterone acetate in breast cancer tissues"Breast Cancer Research and Treatment. 56. 11-23 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] E.Shiba, S.Kim, J.Kambayashi, I.Kawamura, E.Lacey, T.Manda, K.Shimomura, T.Taguchi, S.Takai.: "Mechanism of growth inhibition by calpain inhibito in MCF-7 cells."Anticancer Res.. 17. 1919-1924 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] F.Tsukamoto, E.Shiba, T.Taguchi, T.Sugimoto, T.Watanabe, SJ.Kim, Y.Tanji, Y.Kimoto, M.Izukura, S.Takai.: "Immunohistochemical Detection of P-glycoprotein in Breast Cancer and its Significance as a Prognostic Factor"Breast Cancer.. 1. 259-263 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Sugimoto, E.Shiba, T.Watanabe, S.Takai.: "Suppression of parathyroid hormone-related protein messenger RNA expression by medroxyprogesterone acetate in breast cancer tissues"Breast Cancer Research and Treatment.. 56. 11-23 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugimoto T.et al.: "Suppression of parathyroid hormone-related protein messenger RNA expression by medroxyprogesterone acetate in breast cancer tissues"Breast Cancer Res Treat. 56. 11-23 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ariyoshi H.et al.: "Gradients in cytoplasmic calcium concentration ([Ca^<2+>]_j) in migrating human umbilical vein endothelial cells (HUVECs) stimulated by shear-stress"Life Sciences. 65. 2643-2651 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi S.et al.: "The possible prognostic significance of p53 immunostaining status of the primary tumor in patients developing local recurrence after breast conserving surgey"Oncology. 55. 450-455 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noguchi S.et aet al.: "Clinicopathological analysis of BRCA1-or BRCA2-associated hereditary breast carcinoma in Japanese women"Cancer. 85. 2206-2211 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shiba E.et al.: "Comparison of dual-head coincidence gamma camera FDG imaging with FDGPET in detection of breast cancer and axilary lymph node metastasis"J Nucl Med. 40. 1003-1008 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shiba E.et al.: "Prognostic significance of urokinase type plasminogen activator (u-PA) system in node-negative breast cancer"Breast Cancer. 6. 357-360 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugimoto.S,Watanabe.T,Shiba.E,Takai.S: "SUPPRESSION OF PARATHYROID HORMONE-RELATED PROTAIN MESSEMGER RNA EXPRESSION BY MEDROXYPROGESTERONE ACETATE IN BREAST CANCER TISSUES" Breast Cancer Reseach & Treatment.

    • Related Report
      1998 Annual Research Report
  • [Publications] すぎ本卓司他: "乳癌組織中のDNA-(cytosine-5)-methyltransferase mRNA発現量" 乳癌基礎研究. vol.7. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takuji Sugimoto et.al: "Suppression of Parathyroid Hormon-related Protein Messenger RNA Expression by Medroxy Progesterone Acetate in Breast Cancer Tissues" Breast Cancer Research and Treatment.

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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