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Development for CMV vaccine using the cytotoxic peptide after transplantation

Research Project

Project/Area Number 09671245
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionWakayama Medical School

Principal Investigator

TSUNODA Takuya  Wakayama Medical School, 2nd Dept.of Surgery, Assistant Professor, 医学部, 助手 (30275359)

Co-Investigator(Kenkyū-buntansha) IWAHASHI Makoto  Wakayama Medical School, 2nd Dept.of Surgery, Assistant Professor, 医学部, 助手 (70244738)
YAMAUE Hiroki  Wakayama Medical School, 2nd Dept.of Surgery, Associate Professor, 医学部, 助手 (20191190)
TANIMURA Hiroshi  Wakayama Medical School, 2nd Dept.of Surgery, Professor, 医学部, 教授 (10026990)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsCMV / CTL / peptide / Vaccination / transplantation
Research Abstract

CMV pp65 specific CTL clone was established by limiting dilution method. In order to map the epitope peptide which was restricted by HLA-B35 and specific for CMV pp65, various kinds of truncated pp65 recombinant vaccinia virus were established by homologous recombination. Full (606)-pp65 rVac, 458-pp65 rVac, 383-pp65 rVac, 316-pp65 rVac, 225-pp65 rVac, 122-pp65 rVac and 0-pp65 rVac were established. Mapping was performed by CTL clone against the target infected by various kinds of pp65 truncated recombinant vaccinia virus. It was clarified that the epitope peptide was located from 112 amino acid (aa) to 225 aa from N terminal of pp65 sequence. Furthermore, in order to make sure that epitope peptide was processed on the target cells, a large amounts of target cells were cultured, and were washed by acid buffer (pH2) to remove the peptides from the target cell. By HPLC, this peptides solution was fractionated and applied to LCL (EBV immortalized cell).
For analysis of the cytotoxic activity by CTL clone. It was also clarified that epitope peptide was on the cell surface which binds with MHC molecules.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Takuya Tsunoda et al.: "Clinical efficacy of adoptive immunotherapy by IL-4 activated tumor-infiltrating lymphocytes in patients with advanced cancer"International Joural of Clinical Oncology. 2・4. 202-207 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroki Yamaue et al.: "Clinical effects of chemoimmunotherapy for patients with peritoneal carcinomatosis"Oncology Report. 4. 583-589 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kohhei Noguchi et al.: "Enhancement of tumor cell susceptibility to tumor-infiltrating lymphocytes by cisplatine"Journal of Cancer Research Clinical Oncology. 123. 345-351 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也他: "ペプタイドと臨床免疫「臨床編」"癌治療と宿主. 10・1. 99-104 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也他: "最近のグラム陽性球菌の薬剤耐性化(第1報)・近畿地区におけるMRSAに関するアンケート調査成績"日本感染症学会雑誌. 71. 1-5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也他: "グラム陽性球菌の薬剤耐性における最近の動向(第2報)・近畿地区におけるペニシリン耐性肺炎球菌に関するアンケート調査成績"日本感染症学会雑誌. 71. 6-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwahasi M et al.: "Clinical evaluation of hepatic arterial infusion of low dose-CDDP and 5-FU with hyperthermotherapy : A preliminary study for live metastases from esophageal and gastric cancer"Hepato-Gastroenterology. 46. 2504-2510 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terasawa H et al: "Antitumore effects of linterleukin-2 gene-modified fibroblasts in an orthotopic colon cancer model"Jpn. J. Cancer Res.. 90. 1000-1006 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也 他: "樹状細胞の基礎と臨床.樹状細胞を用いた細部療法 : 消化器癌"最新医学. 54・11. 2682-2688 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也 他: "消化器癌に対する癌ワクチン療法"外科治療. 81・2. 223-225 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也 他: "Methodology癌と感染症,癌患者の術後感染症"Biotherapy. 13・8. 897-902 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 角田卓也 他: "外科分子病態学"医学書院. 350 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunoda T.: "In vitro antitumor effect of Topoisomerase-l inhibitor, CPT-11, on freshly isolated human human gastric and colorectal cancer."Anticancer Res. 19 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Terasawa H.: "Antitumor effects of interleukin-2 gene-modified fibroblasts in an orthotopic colon cancer model."Jpn. J. Cancer Res. 90. 1000-1006 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunoda T.: "Can tumor vaccine induce tumor specific CTL in Cancer patients? -In viewpoints of CEA specific cancer vaccine-"Proceedings of the 11th BACT Symposium. 5-10 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunoda T.: "In vitro augmentation of antitumor effect in combination with CPT-11 and CDDP for human colorectal cancer."Journal of Surgical Oncology. 73 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwahashi M.: "Clinical evaluation of hepatic arterial infusion of low dose-CDDP and 5-FU with hyperthermotherapy : A preliminary study for liver metastases from esophageal and gastric cancer."Hepato-Gastroenterology. 46. 2504-2510 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hotta T.: "P-glycoprotein-expressing tumor cells are resistant to anticancer drugs in human gastrointestinal cancer."Surg. Today. 29. 591-596 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunoda T.: "Tumor specific CTL therapy for advanced cancer and development for cancer vaccine."Hepato-Gastroenterology. 46. 1287-1292 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamaue H.: "Multidisciplinary treatment for gastric cancer patients by chemoimmunotherapy."Hepato-Gastroenterology. 46. 620-625 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hotta T.: "P-glycoprotein expression and chemosensitivity in highly purified fresh human gastrointestinal cancer cells"Hepato-Gastroenterology. 46. 316-321 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishimoto K.: "Function preserving operation for rectal cancer based on preoperative staging by endorectal EUS and MRI by two oblique method."Klinicka Onkologie. 17-19 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsunoda T.: "Clinical efficacy of adoptive immunotherapy by IL-4 activated tumor-infiltrating lymphocytes against advanced cancer patients."Int J Clin Oncol. 2. 202-207 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamaue H.: "Clinical effects of chemoimmunotherapy for patients with peritoneal carcinomatosis."Oncol Rep. 4. 583-589 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hotta T.: "Modulation of multidrug resistance by cepharanthine in fresh human gastrointestinal tumor cells"Oncology. 54. 153-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Noguchi K.: "Enhancement of tumor cell susceptibility to TILs by cisplatin."J Cancer Res Clin Oncol. 123. 345-351 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Noguchi K.: "Cisplatin modulates the tumor cell susceptibility to tumor-infiltrating lymphocytes in vivo."Oncol Rep. 4. 927-930 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hotta T.: "Etoposide enhances the antitumor effects of cisplatin in gastric cancer cells."Anticancer Res. 17. 885-890 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwahashi M et al.: "Clinical evaluation of hepatic arterial infusion of low dose-CDDP and 5-FU with hyperthermotherapy: A preliminary study for liver metastases from esophageal and gastric cancer"Hepato-Gastroenterology. 46. 2504-2510 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Terasawa H et al: "Antitumor effects of interleukin-2 gene-modified fibroblasts in an orthotopic colon cancer model"Jpn.J.Cancer Res.. 90. 1000-1006 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 角田卓也 他: "樹状細胞の基礎と臨床.樹状細胞を用いた細胞療法:消化器癌"最新医学. 54・11. 2682-2688 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 角田卓也 他: "消化器癌に対する癌ワクチン療法"外科治療. 81・2. 223-225 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 角田卓也 他: "Methodology癌と感染症,癌患者の術後感染症"Biotherapy. 13・8. 897-902 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 角田卓也 他: "外科分子病態学"医学書院. 350 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takuya Tsunoda et al.: "Tumor specific CTLtherapy for advanced cancer and development for cancer vaccine" Hepato-Gastroenterology. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 角田卓也: "癌免疫研究の未来-残された最後の挑戦" Newsletter(基盤的癌免疫研究会). 1. 4-5 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 角田卓也他: "外科領域の難治性真菌感染症" 日本医真菌学会雑誌. 39・4. 203-209 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 谷村 弘他: "真菌感染症の早期診断と治療" 外科治療. 79・10. 729-738 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山上裕機他: "胃癌の組織培養法による抗癌剤感受性試験" 癌の臨床. 44. 265-268 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 岩橋 誠他: "B細胞と抗体-遺伝子組み換え型抗体とその応用-" Surgery Frontier. 5・4. 105-112 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takuya Tsunoda et al.: "Clinical efficacy of adoptive immunotherapy by IL-4 activated tummor-infiltrating lymphocytes in patients with advanced cancer" International Journal of Clinical Oncology. 2・4. 202-207 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroki Yamaue et al.: "Clinical effects of chemoimmunotherapy for patients with peritoneal carcinomatosis" Oncology Report. 4. 583-589 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kohhei Noguchi et al.: "Enhancement of tumor cell susceptibility to tumor-infiltrating lymphocytes by cisplatine" Journal of Cancer Research Clinical Oncology. 123. 345-351 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也他: "ペプタイドと臨床免疫「臨床編」" 癌治療と宿主. 10・1. 99-104 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也他: "最近のグラム陽性球菌の薬剤耐性化(第1報).近畿地区におけるMRSAに関するアンケート調査成績" 日本感染症学会雑誌. 71. 1-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 角田卓也他: "グラム陽性球菌の薬剤耐性における最近の動向(第2報).近畿地区におけるペニシリン耐性肺炎球菌に関するアンケート調査成績" 日本感染症学会雑誌. 71. 6-10 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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