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Identification of new oncogenes related to human cancer by human genomic DNA analysis based on two-dimensio electrophoresi

Research Project

Project/Area Number 09671249
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKeio University

Principal Investigator

AIURA Koichi  Keio University School of Medicine Instructor, 医学部, 助手 (00184010)

Co-Investigator(Kenkyū-buntansha) SAITO Junichi  Keio University School of Medicine assistant, 医学部, 助手 (30276274)
ITANO Osamu  Keio University School of Medicine assistant, 医学部・, 助手 (90265827)
UEDA Masakazu  Keio University School of Medicine assistant professor, 医学部, 講師 (50142419)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsmolecular biology / human genome / two-dimensional electrophoresis / oncogene / RLGS method / ヒト癌 / 癌関連遺伝子 / スクリーニング / クローニング
Research Abstract

To evaluate alterations of genomic DNAs related to human cancer, we used restriction landmark genomic scanning (RLGS) based on two-dimensional gel electrophoresis. This method has many benefits, one of which can detect thousands of gene fragments on one X-ray film at one time. This analysis was applied for human colorectal cancer and hepatocellular carcinoma. Genomic alterations specific in those cancer tissues were analyzed and the sequences of the gene fragment were identified. Furthermore, we have clarified the correlation between those genomic alterations and clinical characteristics.
1 . Average twelve changes in DNA spots after two-dimensional gel electrophoresis were seen in a case with colorectal cancer. one of the DNA fragments in the spot was extracted and the nucleotide sequence was determined by ABI PRISM GENETIC ANALYSER.The sequence (410 bps) was found to be highly homologous to human fatty acid syhthase (65 %) in Gene Bank database. The expression of the RNA was also observed in the only cancer tissues by northern blot hybridization using the probe from the DNA spot.
2. Average sixteen changes in DNA spots were seen in a case with hepatocellular carcinoma. The survival rates for patients with <greater than or equal> 16 changed RLGS spots were significantly lower than those for patients with fewer changed RLGS spots ( <less than or equal> 15 spots). Four spots of the DNA alterations of cancer tissues were extracted and sequenced. One (1088 bps) was located at chromosome 8q21 and corresponded to human tandem repeat sequence. Another one (1415 bps) was centromeric NotI cluster. The disease-free survival rates for patients with hepatocellular carcinoma were closely associated with the intensity of those

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 上田政和: "RLGS法による大腸癌の臨床特性を反映する遺伝子のスクリーニング" Mebio. 14. 87-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda Masakazu: "Screening of genomic DNA alterations specific in human colorectal cancer by RLGS" Mebio. 14. 87-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 上田政和: "RLGS法による大腸癌の臨床特性を反映する遺伝子のスクリーニング" Mebio. 14. 87-90 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.AIURA: "Interaction with autologous platelets multiplies IL-1 and TNF production in mononuclear cells." J.Infoct.Dis.175. 123-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 相浦 浩一: "経皮経肝胆道鏡検査で確認しえた再発総胆管結石を合併した乳頭括約筋形成術後の下部胆管癌の1例" 消化器内視鏡の進歩. 50. 320-321 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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