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Induction of Tumor-specific Cytotoxic Tlymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer

Research Project

Project/Area Number 09671269
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

YOSHINO Ichiro  University of Occupational and Environmental Health, Medical Department Research Associate, 医学部, 助手 (40281547)

Co-Investigator(Kenkyū-buntansha) ICHIYOSHI Yuji  University of Occupational and Environmental Health, Medical Department Assistan, 医学部, 講師 (50264031)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBreast cancer / Cytotoxic T lymphocyte / Regional lymph node lymphocyte / HLA-A2 / Tumor antigen / Major histocompatibility antigen / 主要組織適合抗原 / 細胞障害性T細胞 / 癌抗原
Research Abstract

In this study, we have induced the breast cancer-specific cytotoxic T lymphocytes (CTL) from regional lymph node lymphocytes (RLNL) of HLA A2-positive patients with breast cancer. Freshly isolated RLNL were stimulated with solid phase anti-CD3 monoclonal antibody followed by expansion with recombinant interleukin-2. Subsequently, the RLNL were stimulated with an irradiated HLA 0201 breast cancer cell line, MCF-7, at responder/stimulator ratio of 10/1 once a week for 2 weeks. The cultured RLNL exhibited specific lysis against MCF-7 in all 5 HLA A2-positive patients tested, but not in 2 HLA A2-negative patients. The cytotoxicity against MCF-7 was substantially inhibited by an addition of anti-HLA A2 mAb. In 3 out of the 5 HLA A2-positive patients, the anti-MCF-7 CTL also exhibited a substantial level of reactivity against PC-9, an HLA A0206-positive lung adenocarcinoma cell line. Inversely, anti-PC-9-speciflc CTL was inducible by multiple stimulations of the RLNL with PC-9 cells in 2 out of the 3 patients. These results suggested that several common tumor antigens might exist among HLA A2-positive breast cancers, some of which may be shared with lung adenocarcinomas.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ryozo Eifuku: "Induction of Tumor-specific Cytotoxic Tlymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer" Breast cancer. 5. 367-373 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ryozo Eifuku, Ichiro Yoshino, et al.: "Induction of Tumor-specific Cytotoxic T lymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer" Brerast cancer. 5. 367-373 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ryozo Eifuku: "Induction of tumor-specific cytotoxic T lymphocytes from regional lymph node lymphocytes of human bre can" Breast Cancer. 5. 367-373 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshino et al.: "The induction of cytotoxic T lymphocytes against HLA-A locus-matched long adenocarclnema in patlents with mon-small ceil lung cancer" Japanese Journal of Cancer Research. 88. 743-749 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshino et al.: "Postoperative plognosis in patiients with mon-small ceil lung cancer with synchronous intrapulmencry metastases" Annals of Thoracic Surgery. 64. 809-813 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takenoyama et al.: "Autologous cytotoxic T lymphocytes against human lung adenocarclnema recognize shared antigens presented byHLA-A24" Japanese Journal of Cancer Research. 89. 60-66 (1988)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshimatsu et al.: "Expression of melanoma antigen-encoding gene in human lung cancer" Journal of Surgical Ohcology. 67. 126-129 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshimatsu et al.: "Human lung cancer antigen presentation by tumor-assoolated macrophages to CD4^+ lymphocytes derived from regional lymph node" Annals of Cancer Research and Therapy. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hanagiri et al.: "Effect of interleukin-12 on the induction of cytotoxic T lymphocytes from the regional lymph node lymphocytes of patients with lung cancer" Japanese Journal of Cancer Research. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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