| Project/Area Number |
09671284
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAMINISHI Michio The Univ. of Tokyo, Branch Hospital, Professor, 医学部・附属病院分院, 教授 (30126031)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Nobuyuki The Univ. of Tokyo, Branch Hospital, Physician, 医学部・附属病院分院, 医員 (70262128)
NOMURA Sachiyo The Univ. of Tokyo, Branch Hospital, Assistant, 医学部・附属病院分院, 助手 (70301819)
YAMAGUCHI Hirokazu The Univ. of Tokyo, Branch Hospital, Assistant, 医学部・附属病院分院, 助手 (00242149)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Stomach / Gastric cancer / Clonality / Gastric gland / X-chromosome inactivation / Precancerous lesion / X-chromosome methylation / Intestinal metaplasia / 前癌・病変 |
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| Research Abstract |
We tried to pick up glandular cells under a stereomicroscope. But it was impossible to take off all the interstitial tissues. So we decided to analyze clonality of single glands isolated by EDTA method.
As the results, at least, 40% of fundic glands were polyclonal and 7% of pyloric glands were polyclonal. These results were different from results reported before, which all the single glands in gastrointestinal tructs were monoclonal. At least, 50% of single intestinal metaplastic glands were polyclonal, regardless of their histological types or locations.
We employed a transgenic mouse line whose clonality can be seen in situ. and analyzed the clonality of gastric mucosa. There were polyclonal glands on the murine stomach. And there were some monoclonal patches on the mucosa. These patches grow larger as the mice grow older. The mice were administered carcinogenen, but the patch did not become larger.
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