| Project/Area Number |
09671287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
SUZUKI Tsutomu Niigata University, School of Medicine The First Department of Surgery, Associate Professor, 医学部, 助教授 (40183420)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMAKI Tadashi Niigata University, School of Medicine University Hospital, The First Department of Surgery, Lecturer, 医学部・附属病院, 講師 (70242427)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Gastric Cancer / Peritoneal Relapse / Intraperitoneal Cancer Cells / Telomerase Activty / Polymerase Chain Reaction / telomeric repeat amplification protocol (TRAP) assay |
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| Research Abstract |
The aim of this study was to investigate a minute amount of cancer cells which possibly remain in the peritoneal cavity of the patients with gastric cancer even after radical surgery. The telomeric repeat amplification protocol (TRAP) assay was employed for this purpose, and its sensitivity for detection of minute cancer cells was compared to that of the Papanicolau's cytology with a prospect that TRAP assay had a higher sensitivity than the latter method. A total of 103 specimens were collected for survey ; 78 were obtained by saline irrigation of the left subphrenic, right subhepatic and Douglas' cavities at surgery, 23 were from the postoperative drainage, and 2 were from pleural effusion drained from one patient with carcinomatous pleuritis. TRAP assay revealed telomerase activity in only 3 of 23 (13%) specimens which were positive to the cytological examination : a disappointing results. The possible causes of this results were thought that 1) inactivation of telomerase activity during the THRA assay process such as repeated deep freezing and melting of the specimens and contamination of proteases into them, and 2) hyperdilution of the specimens to avoid PCR interference by high concentration of protein fraction. On the other hand telomerase activity was detected in 4 of 80 specimens negative to cytology : an encouraging result which was expected at the start of this study. Hereafter, the clinical course of 2 patients who had telomerace active but cytology negative specimens obtained at surgery should be closely follow-uped. If these patients manifest peritoneal relapse, detection of telomerase activity will have significant importance to estimate probability of peritoneal relapse after surgery.
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