Analysis of the mechanisms of down-regulation of signal transducing CD3 zeta molecules on T cells in patients with cancer-Its correlation with surgical stress.
| Project/Area Number |
09671292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY |
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Principal Investigator |
SEKIKAWA Takayoshi Yamanashi Medical University, 1st Dept.of Surgery, Associate Professor, 医学部, 助教授 (10134553)
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| Co-Investigator(Kenkyū-buntansha) |
KONO Koji Yamanashi Medical University, 1st Dept.of Surgery, Assistant Professor, 医学部, 助手 (40283204)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | surgical stress, / T cell function |
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| Research Abstract |
In cancer patients, we evaluated the alteration of signal transducing CD3 zeta molecules on T cells induced by surgical stress. PBLs were taken preoperatively, 2nd, 3rd and 7th postoperative days(POD). The levels of CD3 zeta molecules on peripheral T cells were quantified by flow cytometry with intracellular staining. The expression of CD3 zeta molecules was down-regulated on 2nd and 3rd POD compared to preoperative levels. Then, decreased expression of CD3 zeta molecules induced by surgical stress were restored to the preoperative levels on 7th POD.Co-culture of monocyte on 2nd POD with PBLs from preoperative periods induced down-regulation of CD3 zeta, indicating that surgical stress induced down-regulation of CD3 zeta molecules on T cells, which were mediated by monocyte. Moreover, preliminary results indicated that monocyte on 2nd POD induced apoptosis of T cell, however, further investigation need to prove this phenomenon.
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Report
(3 results)
Research Products
(6 results)
