| Project/Area Number |
09671293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY |
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Principal Investigator |
MATSUMOTO Yoshirou FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY,DEPARTMENT OF SURGERY,PROFESSOR, 医学部, 教授 (20159156)
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| Co-Investigator(Kenkyū-buntansha) |
FUJI Hideki FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY,DEPARTMENT OF SURGERY,ASSOCIATE, 医学部, 講師 (30181316)
KOBAYASHI Masashi FACULTY OF MEDECINE,YAMANASHI MEDICAL UNIVERSITY,DEPARTMENT OF SURGERY,ASSISTANT (00273045)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Tumor necrosis factor / Liver macrophage / Gadolinium chrolide / splenectomy / splenic macrophage / endotoxin |
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| Research Abstract |
We investigated the function of splenic macrophages (Mphis) with respect to cha nges in plasma tumor necrosis factor (TNF) in lethally endotoxemic rats treated with gadolnium chloride (GdCI3), which blocks the phagocytosis of large liver Mphis. We also carried out an immunohistochemical study to investigate the change of populations of liver and splenic Mphis under the condition of dysfunction of liver Mphis with or without splenectomy. Twenty-four-hour survival rates were 100% in the GdCl3-treated group (n=6) and 0% in the non-treated group (n=6). These rates did not change with splenectomy. Immunohistochemical examination with the primary monoclonal antibodies ED1, ED2 and ED3 revealed that large liver Mphis were eliminated after GdCL3 injection, and that this was not related to splenectomy, The splenic Mphis were not affected by GdCI3 treatment.
Plasma TNF levels did not differ between the GdCI3-treated and the nontreated groups, irrespective of whether splenectomy was performed. It was suggested that plasma TNF levels are not affected by the splenic Mphis and that they do not compensate for dysfunction of liver Mphis after GdCl3 treatment.
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