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Research on the prediction of hematogenous metastasis and recurrence of hepatocellular carcinoma, pancreatic carcinoma, gastric carcinoma and esophageal carcinoma. (Detection of circulating AFPmRNA,CEAmRNA and Cytokeratin mRNA)

Research Project

Project/Area Number 09671301
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SHIMADA Yutaka (1998)  Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical Basic Science, Assistant Professor, 医学研究科, 講師 (30216072)

宮原 とき治 (1997)  京都大学, 医学研究科, 助手 (30273452)

Co-Investigator(Kenkyū-buntansha) IMAMURA Masayuki  Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical, 医学研究科, 教授 (00108995)
嶋田 裕  京都大学, 医学研究科, 講師 (30216072)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCirculating tumor cells in blood, / SCCmRNA, / CEAmRNA / AFPmRNA, / Cytokeratin20mRNA / 血中浮遊食道癌 / Cytoteratin 20mRNA / Cytokeratin 20mRNA
Research Abstract

In this study, we established detection system (RT-nested PCR) of various gastrointestinal carcinoma cells in blood in the form of following mRNA.Alpha-fetoprotein(AFP) mRNA for hepatocellular carcinoma, carcinoembrionic carcinoma antigen (CEA) mRNA for pancreatic and gastric carcinoma, cytokeratin 20mRNA for colorectal carcinoma, and squamous cell carcinoma antigen (SCC) mRNA for esophageal carcinoma These systems could detect one to five cancer cells in 1ml of blood and magnetic cell absorption followed by immunohistochemistry clearly demonstrated the cells, which express CEA or Cytokeratin in peripheral blood. We also detected CEA mRNA in portal vein during operations in pancreatic carcinoma patients and SCC mRNA in peripheral blood during operations in patients with esophageal carcinoma. These facts show that carcinoma cells can be disseminated in blood during operations.
Detection of carcinoma related mRNAs in blood correlated with patients prognosis and recurrence. There were patients whose CEA mRNA or SCC mRNA was not detected after chemotherapy, however the protocols of drug administration remains controversial. Our detection systems of cancer cells in blood are useful for prediction of patients' prognosis and might have beneficial information for strategy of chemotherapy. Clinicopathological as well as molecular biological analysis of the patients with positive cancer cells in blood should be evaluated in the future.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Shimada Y: "Genetic Alterations in Patients with Esophageal Cancer with Short and Long Term Survival Rates after Curative Esophagectony" Ann Surg. 226. 162-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y: "Prognostic factors of oesophageal squamous cell carcinoma from the perspective of molecular biology" Br J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y: "Esophageal cancers Human Cell Culture" J. R. W. Masters and B. Palsson (eds) Kluwer Academic publishers., 34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 嶋田 裕: "消化器病セミナー69 食道癌-診断と治療の最新の進歩" へるす出版, 21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y.: "Genetic Alterations in Esophageal Cancer Patients with Short and Long Term Survival Rates after Curative Esophagectomy." Ann Surg. 226. 162-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y.: "Prognostic factors of oesophageal squamous cell carcinoma from the perspective of molecular biology." Br J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y.: Esophageal cancers.Human Cell Culture J.R.W.Masters and B.Palsson (eds) Kluwer Academic publishers., 179-212 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimada Y.: Genetic diagnosis and tumor marker of the esophageal cancer.Seminar of Gastroenterology 69 Esophageal cancer, Sugimachi K ed., Herusu Shuppan, 49-69 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naomi Omichi Funaki: "Cyto Keratin 20mRNA in peripheral venous blood of colorectal carcinoma patients" Br J Cancer. 77 (8). 1327-1332 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naomi Omichi Funaki: "Quantitive analysis of Carcinoembryonic antigen mRNA in peripheralvenous blood and parlal blood of patients with pancreatic ductal adenocarcinama" Clinical cancer research. vol 4. 855-860 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimada Y: "Prognostic factors of oesaphageal Squamous cell carcinoma from the perspective of molecular biology" Br J Cancer. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shimada Y: "Esophageal cancers Human Cell Culture" Kluwer Academic publishers, 34 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 嶋田 裕: "食道癌の遺伝子診断" 外科. 59. 929-93 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomi Omichi Funaki: "Cytokeratin 20mRNA in peripheral Venous blood of colorecta carcinoma patients." Br J Cancer. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomi Omichi Funaki: "Quantitive analysis of Carcinoembryonic antigen mRNA in peripheral venousblood and portal blood of patients with pancreatic ductal adenocarcinoma" Clinical cancer research. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomi Omichi Funaki: "Hematogenous spreading of hepatocellular carcinama cells:possible participation in recurrence in the liver" Hepatology. 25. 564- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomi Omichi Funaki: "Detection of colorectal carcinoma cells in circulating peropheral blood by reverse transcription=polymerase chain reaction targetin cytokeratin-20mRNA" Life Science. 60. 643- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 嶋田 裕: "消化器病セミナー 69" 分子生物学的手法を用いた診断(遺伝子診断、腫瘍マーカー), 20 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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