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Research for tight-junction assatiated protein in human digestive tract.

Research Project

Project/Area Number 09671303
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOsaka University

Principal Investigator

INOUE Masatoshi  Osaka University Medical School, Assistant Professor, 医学部, 助手 (80232560)

Co-Investigator(Kenkyū-buntansha) DOKI Yuishiro  Osaka University Medical School, Assistant Professor, 医学部, 助手 (20291445)
YANO Masahiko  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70273646)
TSUJINAKA Toshimasa  Osaka University Medical School, Lecturer, 医学部, 講師 (40188545)
SHIOZAKI Hitoshi  Osaka University Medical School, Associate Professor, 医学部, 助教授 (70144475)
五福 淳二  大阪大学, 医学部・附属病院, 医員
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordstight junction / occludin / ZO-1 / cancer / digestive tract / differentiation / タイトジャンクション / 消化器癌
Research Abstract

The tight junction seals cells together at a subapical location and functionally separates the plasma membrane into an apical and a basolateral domain.This junction is one of the most characteristic structural markers of the polarized epithelial cell.Recently, occludin has been identified as an integral transmembrane protein localizing at the tight junction and directly associated with ZO-1, an undercoat-constitutive cytoplasmic protein.We have investigated occludin expression in conjunction with ZO-1 in normal epithelia and cancers of human digestive tract by immunostaining with a new antibody raised against human occludin.In the normal simple columnar epithelium, occludin was expressed together with ZO-1 as a single line at the apical cell border.However, in the esophagus, which has a stratified squamous epithelium, no occludin expression could be detected, but ZO-1 was expressed in the spinous layer.As for cancers, both occludin and ZO-1 showed the same expression in differentiated adenocarcinoma cells as in normal epithelium, but in poorly differentiated adenocarcinomas, the expression of these two proteins was reduced.There was significant correlation between tumor differentiation and expression of these proteins.These results suggest that occludin, together with ZO-1, is involved in the formation of gland-like structures.In addition, occludin expression can serve as a histopathological indicator for differentiation in gastrointestinal enocarcinomas.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Gofuku J.: "Expression of E-cadherin and α-catenin in patients with colorectal carcinoma. Correlation with cancer invasion and metastasis." Am.J.Clin.Pathol. 111. 29-37 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takayama T.: "Abberant expression and phosphrylation of β-catenin in human colorectal cancer." Br.J.Cancer. 77. 605-613 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gofuku J.: "Characterization of soluble E-cadherin as a disease marker in gastric cancer patients." Br.J.Cancer. 78. 1095-1011 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shamma A.: "Effect of cyclin D1 and associated proteins on proliferation of esophageal squamous cell carcinoma." Int.J.Oncol.13. 455-460 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura Y.: "Expression of Occludin,Tight-Junction-Associated Protein,in Human Digestive Tract" Am.J.Pathol. 151. 45-54 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue M.: "Study on the Post-Operative Adjuvant Chemotherapy in Gastric Cancer Patients Undergoing Curative Resection (Histological Stages II and III)" Proceedings of the 2nd International Gastric Cancer Congress. 1. 1771-1775 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gofuku J., Shiozaki H., Tsujinaka T., Inoue M., Tamura S., Doki Y., Matsui S., Tsukita S., Kikkawa N., Monden M.: "Expression of E-cadherin and alpha-catenin in patients with colorectal carcinoma.Correlation with cancer invasion and metastasis." Am.J.Clin.Pathol. 111. 29-37 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takayama T., Shiozaki H., Doki Y., Oka H., Inoue M., Yamamoto M., Tamura S., Shibamoto S., Ito F., Monden M.: "Abberant expression and phosphylation of beta-catenin in human colorectal cancer." Br.J.Cancer. 77. 605-613 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gofuku J., Shiozaki H., Doki Y., Inoue M., Hirano M., Fukuchi N., Monden M.: "Characterization of soluble E-cadherin as a disease marker in gastric cancer patients." Br.J.Cancer. 78. 1095-1011 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shamma A., Doki Y., Shiozaki H., Tsujinaka T., Inoue M., Yano M., Kimura Y., Yamamoto M., Monden M.: "Effect of cyclin D1 and associated proteins on proliferation of esophageal squamous cell carcinoma." Int.J.Oncol.13. 455-460 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura Y., Shiozaki H., Hirao M., Maeno Y., Doki Y., Inoue M., Monden T., Ando-Akatsuka Y., Furuse M., Tsukita S., Monden M.: "Expression of Occludin, Tight-Junction-Associated Protein, in Human Digestive Tract" Am.J.Pathol. 151. 45-54 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue M., Shiozaki H., Furukawa H., Hiratsuka M., Iwanaga T., Mori T., Kawahara T., Noguchi S., Takami M., Tsujinaka T., Kido Y., Murata A., Suzuki T., Monden M.: "Study on the Post-Operative Adjuvant Chemotherapy in Gastric Cancer Patients Undergoing Curative Resection (Histological Stages II and III)" Proceedings of the 2nd International Gastric Cancer Congress. 1771-1775 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gofuku J.: "Expression of E-cadherin and α-catenin in patients with colorectal carcinoma. Correlation with cancer invasion and metastasis." Am.J.Clin.Pathol. 111. 29-37 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takayama T.: "Abberant expression and phosphrylation of β-catenin in human colorectal cancer." British J Cancer. 77. 605-613 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gofuku J.: "Characterization of soluble E-cadherin as a disease marker in gastric cancer patients." British J Cancer. 78. 1095-1011 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shamma A.: "Effect of cyclin D1 and associated proteins on proliferation of esophageal squamous cell carcinoma." lnt.J.Oncol.13. 455-460 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimura-Y: "Epression of occludin tight-junction-associated protein,in human digestive tract." Am-J-Pathol.151(1). 45-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Doki-Y: "Increased expression of the p27Kipl protein in human esophageal cancer cell line over-express cyclin D1." Carcinogenesis. 18(6). 1139-1148 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takayama-T: "Aberrant expression and phosphorylation of β-catenin in human colorectal cancer." Br-J-Cancer.77(4). 605-613 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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