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Novel cancer gene therapy using apoptosis-related gene

Research Project

Project/Area Number 09671304
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOsaka University

Principal Investigator

SHIMIZU Shigeomi  Osaka University Medical School, assistant professor, 医学部, 助手 (70271020)

Co-Investigator(Kenkyū-buntansha) EGUCHI Yutaka  Osaka University Medical School, associate professor, 医学部, 助教授 (20243206)
上池 渉  大阪大学, 医学部, 助教授 (40152847)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordscaspase-3(CPP32 / Yama) / Bcl-2 / apoptosis / yama / cpp32
Research Abstract

Caspase-3 is a member of the cysteine protease family, which plays a crucial role in apoptosis.We applied the human caspase-3 gene as a novel form of anticancer gene therapy.Overexpression of human caspase-3 alone could not induce apoptosis of tumor cell lines, whereas apoptosis was markedly enhanced by the addition of VP-16.In an AH13O liver tumor model, transfection of human caspase-3, but not the empty vector, induced extensive apoptosis and reduced tumor volume, when applied together with VP-16.However, these effect was not observed with a Bcl-2 overexpressing tumor.In conclusion, caspase-3 gene transfection accompanied by an additional death stimulus may be useful anticancer gene therapy, except for Bcl-2 overexpressing tumors.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] K.Yamabe: "Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection" Biochem.Biophys.Res.Commun.243. 217-223 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Shimizu: "Bcl-2 prevents mitochondrial potential loss by regulating ion flux" Proc.Natl.Acad.Sci.USA. 95. 1455-1459 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Okuno: "Bcl-2 prevents caspase-independent cell death" J.Biol.Chem.273. 34272-34277 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 清水重臣: "アポトーシスの分子機構" 生化学. 70. 14-21 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Yamabe: "Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection" Biochem.Biophys.Res.Commun.243. 217-223 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Shimizu: "Bcl-2 prevents mitochondrial potential loss by regulating ion flux" Proc.Natl.Acad.Sci.USA. 95. 1455-1459 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Okuno: "Bcl-2 prevents caspase-independent cell death" J.Biol.Chem.273. 34272-34277 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Shimizu: "Molecular mechanism of apoptosis" Jpn.J.Biochemistry. 70. 14-21 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Yamabe: "Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection." Biochem.Biophys.Res.Commum.243. 217-223 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Shimizu: "Bcl-2 prevents mitochondrial potenmtial loss by regulating ion flux." Proc.Natl.Acad.Sci.USA. 95. 1455-1459 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Okuno: "Bcl-2 prevents caspase-independent cell death." J.Biol.Chem.273. 34272-34277 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 清水重臣: "アポトーシスの分子機構" 生化学. 70. 14-21 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Ohtsu: "Inhibition of apoptosis by an actin-regulatory protein gelsolin" EMBO Journal. 16. 4650-4656 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Yamabe: "Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection" Biochem.Biophys.Res.Commun.243. 217-223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Shimuzu: "Bcl-2 prevents mitochondrial potential loss by regulating ion flux" Proc.Natl.Acad.Sci.USA. 95. 1455-1459 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Tanabe: "Death-signaling cascade in mouse cerebellar granule neurons" Eur.J.Neurosci.in press (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 清水重臣: "Bcl-2ファミリーとICEファミリープロテアーゼによる細胞死の制御" 箱根シンポジウム. 7. 2-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 清水重臣: "アポトーシスの分子機構" 生化学. 14-21 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 清水重臣: "アポトーシス研究の新展開" bcl-2によるアポトーシス抑制機構, 5 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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