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Analysis of pathophysiology of bacterial translocation under surgical stress.

Research Project

Project/Area Number 09671306
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKobe University

Principal Investigator

USAMI Makoto  Kobe University, School of Medicine, Professor, 医学部, 教授 (00193855)

Co-Investigator(Kenkyū-buntansha) 山本 正博  神戸大学, 医学部, 助教授 (40166822)
笠原 宏  神戸大学, 医学部・付属病院, 助手 (10243304)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordssurgical stress / intestinal mucosal integrity / diamine oxidase / IL-6 / bacterial translocation / enteral nutrition / dietary nucleotide deficiency / ω 3 polyunsaturated fatty acids / bacterial tlanslocation / 食餌生核酸欠乏 / ω3 / 腸粘膜機能 / diamine bxidas 活性 / エンドトキシンシ / 1L-6 / diamine oxidase活性 / エンドトキシン / lL-6 / lL-12
Research Abstract

Bacterial translocation is a major concern as a cause developing sepsis or multiple organ failure. However the relationship between intestinal mucosal integrity and systemic inflammatory response syndrome (SIRS) or hypercytokinemia has not been evaluated after abdominal surgery. In this study, pathophysiology of decreased intestinal integrity and potential therapeutic modality was evaluated in clinical and experimental studies.
1)Postheparin plasma diamine oxidase (DAO) activity (PHD activity), indicator of intestinal mucosal integrity, decreased in patients shortly after major abdominal surgery (esophagectomy, pancreatectomy, total gastrectomy). Negative correlation between decreased PHD activity and elevated blood pro-inflammatory cytokine, interleukin 6 (IL-6), level or SIRS suggested the relationship between them. 2)In an in vitro study using Caco-2 monolayer cells, a model of intestinal mucosal epithelium, addition of IL-6 in the medium directly decreased DAO activity and stimulate … More d E. coli passage across cells by transcellular route with intact nuclear and cytoplasmic structure. The results suggest the direct mechanism of decreased intestinal integrity under surgical stress and to develop bacterial translocation. 3)Enteral feeding, supplemented with dietary fiber and branched chain amino acids, in comparison without EN facilitated recovery of decreased PHD activity after surgery indicating the effectiveness to improve intestinal mucosal integrity. 4)As a nutritional substrate of enteral feeding, two different nutritional substrate were evaluated. The importance of dietary nucleotide to keep adhesional molecule (Mac-1, LFA-1) and oxidative burst in blood neutrophil function in mice was shown. Eicosapentaenoic acid, one of omega 3 polyunsatureted fatty acid, changed on tight junction permeability using Caco-2 monolayer cells in comparison with omega 6 or 9 polyunsaturated fatty acid. These results would provide new insights of pathophysiology and future immunonutrition to keep intestinal integrity. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Usami, M., et al.: "Interleukin-6 facilitates E coli transcytosis in the Caw-2 cell"Bulletin of Health Science Kobe. 15. 35-43 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kotani, J., Usami, M., et al.: "Enteral nutrition prevents bacterial translocation but …"Arch Surg.. 134 (4). 287-292 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, Y., Usami, M., et al.: "Endothelin in liver cell injury and regeneration after TOY, hepateotlomy …"J. Cardiovascular Pharmacology. 31. S480-S481 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 平井昭博,宇佐美真,他: "食餌性核酸欠乏下の好中球機能変化と核酸成分液投与効果"外科と代謝・栄養. 32(4). 259-266 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 宇佐美眞,他: "深在性真菌症の病態と治療"外科治療. 78(4). 409-416 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirai, A., Usami, M., et al.: "Purine and Pyrimidine Metabolism in Man IX"Changes of polymorphonuclear leukocyte (PMN) functions …. 543-547 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Interleukin-6 facilitates Escherichia coli transcytosis in the Caco-2 cell monolayer system"Bulletin of Health Science Kobe. 15. 35-43 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kotani J, Usami M, et al.: "Enteral nutrition prevents bacterial translocation but does not improve survival during acute pancreatitis"Arch Surg. 134. 287-292 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura Y, Usami M, et al.: "Endothelin in liver cell injury and regeneration after 70% hepatectomy with portal ischemia"J Cardiovascular Pharmacology. 31(Suppl 1). S480-481 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirai A, Usami M, et al.: "Changes of neutrophil functions under dietary nucleic acid deficiency and effect of nucleotide and nucleosides mixture solution administration in mice"Jap J Surg Metab Nutri. 32(in Japanese). 259-266 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Deep seated mycosis in patients after surgery"Surgical Therapy. 78(in Japanese). 409-416 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Plasma postheparin diamine oxidase activity after major abdominal surgery"Bulletin of Health Science Kobe. 13. 47-55 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirai A, Usami M, et al.: "Up-to date in enteral nutrition in patients after esophagectomy"JJPEN. 19(in Japanese). 363-366 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Postheparin plasma diamine oxidase activity under systemic inflammatory response syndrome (SIRS) after major abdominal surgery"Jap J Surg Metab Nutri. 31(in Japanese). 307-314 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Evaluation of postoperative plasma diamine oxidase activity and endotoxin level in patients after gastrointestinal surgery"JJPEN. 19(in Japanese). 667-671 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M, et al.: "Energy metabolism and nutritional managements in patients with sepsis"Surgical Therapy. 76(in Japanese). 172-178 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Usami M,et al.: "Interleukin-6 facilitates Escherichia coli transcytosis in the Caco-2 cell monolayer system"Bulletin of Health Scienece Kobe. 15. 35-43 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kotani J,Usami M,et al.: "Enteral nutrition prevents bacterial translocation but does not improve survival during acute pancreatitis"Arch Surg. 134. 287-292 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitamura Y,Usami M,et al.: "Endothelia in liver cell injury and regeneration after 70% hepatectomy with portal ischemia"J Cardiovascular Pharmacology. 31. S480-481 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平井昭博,宇佐美眞,他.: "食餌性核酸欠乏下の好中球機能変化と核酸成分液投与効果の実験的研究"外科と代謝・栄養. 32. 259-266 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美眞,他.: "深在性真菌症の病態と治療"外科治療. 78. 409-416 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Usami M,et al.: "Plasma posthepain diamine oxidase activity after major abdominal surgery"Bulletin of Health Scienece Kobe. 13. 47-55 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平井昭博,宇佐美眞,他.: "食道癌手術におけるENの現状と問題点"JJPEN. 19. 363-366 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美眞,他.: "消化器術後のヘパリン投与後血中ジアミンオキシダーゼ(PHD)活性と血中エンドトキシン値"JJPEN. 19. 667-671 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美眞,他.: "消化器手術後早期の侵襲反応と血中postheparin diamine oxidase活性変化"外科と代謝・栄養. 31. 307-314 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美眞,他.: "敗血症におけるエネルギー代謝と栄養管理"外科治療. 76. 172-178 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirai A,Usami M,et al.: "Purine and Pyrimidine Metabolism in Man IX."Griesmacher A,Chiba P,Muller MM.Plenum Press. 866(543-547) (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美眞,: "gut decontamination 「臨床侵襲学」"小川道雄・斎藤英昭編 へるす出版. 680(568-571) (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宇佐美 眞、他: "敗血症におけるエネルギー代謝と栄養管理" JJPEN. 19. 667-671 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宇佐美 眞、他: "plasma postheparin diamine oxidase activity after major abdominal surgery" Bulletin of Allied Medicel Ssieuces Kobe. 13. 47-55 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宇佐美 眞: "腸管不全と肺障害" 医学のあゆみ. 186. 861-862 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宇佐美 真: "消化器術後のヘバリン投与後血中ジアミンオキシダーゼ(PHD)活性と血中エンドトキシン値" JJPEN. 19・7. 667-671 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宇佐美 真: "消化器手術後早期の侵襲反応と血中postheparin diomine oxidue活性変化" 外科と代謝・栄養. 31・5. 307-314 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宇佐美 真: "臨床侵襲学" 小川道雄、斉藤英昭、へるす出版, 680 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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