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Roles of cytokines for liver regeneration in hepatectomy rat models with postoperative infection

Research Project

Project/Area Number 09671311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YOKOYAMA Takashi  HIROSHIMA UNIVERSITY MEDICAL HOSPITAL PROFESSOR, 医学部・附属病院, 教授 (60034104)

Co-Investigator(Kenkyū-buntansha) SHINBARA Kanae  HIROSHIMA UNIV.M MEDICAL HOSPITAL RESIDENT, 医学部・附属病院, 医員
TAKESUE Yoshio  HIROSHIMA UNIVERSITY MEDICAL HOSPITAL ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (70197292)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Keywordsliver regeneration / endotoxin / hepatectomy / cytokine / HGF / c-met / PCNA / inflammation
Research Abstract

1 : Hepatic regeneration after hepatectomy and endotoxin administration
Postoperative infection inhibits liver regeneration after hepatectomy. To elucidate this inhibition, we analyzed growth rates of hepatocyte and signals between Kupffer cells and hepatocyte using rat 70% hepatectomy model with or without endotoxin (ET) administration. In this rat postoperative infection model, we administered ET (1 mg/kg) one day after hepatectomy. To evaluate hepatocyte growth rate, PCNA (proliferative core nuclear antigen) staining was performed in liver specimens obtained at 3 and 7 postoperative days. The percentages of PCNA positive cells were 27% and 17% in the rats with only hepatectomy whereas those were 16% and 10 % in 1 mg/kg ET-administered rats, respectively. Thus, ET administration inhibited liver regeneration after hepatectomy.
2. Molecular analysis for signalings of hepatic regeneration
Using the same hepatectomy model rats with low dose ET (30 mug/kg)and high dose ET (1 mg/kg)-administe … More red rats, we measured HGF (hepatocyte growth factor) mRNA and c-met mRNA expressions in the liver specimens obtained 3,6,12,24,48 and 72 hours after hepatectomy. in the rats with only hepatectomy, HGF expression increased 12-24 hours after hepatectomy and c-met expression simultaneously increased. In the low dose ET administered rats, the expression levels of HGF and c-met were higher than those in the rats without ET-administration. On the other hand, in the high dose ET administered rats, peak of HGF expression was delayed and unlikely to cooperate with c-met expression.
These data suggested that low dose administration of ET after hepatectomy stimulated hepatic regeneration after hepatectomy, whereas its high dose administration inhibited. ET administration stimulated inflammatory cytokines by Kupffer cells. The overproduction of inflammatory cytokines due to postoperative severe infection might disarrange signal transduction for hepatocyte growth, which causes the delay of liver regeneration and increases the death rates of these rats model. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Takesue Y: "The effectiveness of perineal rectosigmoidectomy for treatment of rectal prorapse in elderly and high-risk patients." Surg Today. 29・3. 290-293 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takesue Y: "Prediction for the development of postoperative infections in the operation of esophageal cancer compared with gastric surgery." Hiroshima J Med Sci.47・3. 109-113 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "消化管不全" 救急医学. 22・13. 1954-1958 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 村上義昭: "各種侵襲下におけるサイトカインと臓器障害との関連" Biotherapy.12・5. 604-606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "加齢に伴う変化-免疫機能(-感染防御能を中心に-)" 老年消化器病. 10・2. 111-117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "術後臓器障害の発生から見た消化器外科一次感染症" Jap J Antibiot.51. 541-542 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "感染症ショック" 消化器外科(新術前・術後管理マニュアル). 21・5. 935 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Asahara T: "Living related partial liver transplantation for primary biliarycirrhosis-a case report." Hiroshima J Med Sci.47・1. 31-37 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kodama T: "Postoperative enteritis caused by methicillin-resistant Staphylococcus aureus." Surg Today. 27. 816-825 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takesue Y: "Surgical treatment for duodenum involvement in Crohn's disease:report a case." Surg Today. 27. 858-862 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山東敬弘: "サイトカイン誘導能よりみた消化器外科術後MRSA腸炎起炎菌の実験的検討" 日消外会誌. 30・7. 1734-1738 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 村上義昭: "末梢好中球数とサイトカインおよび臓器障害との関連" 浸襲と免疫. 6・1. 17-19 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 竹末芳生: "Bacterial translocationの新しい概念とSIRSにおけるその役割" 医学のあゆみ. 181・1. 47-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "臨床医のための救急疾患キーワード" 小川道雄編, 47-51 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "臨床浸襲学" 小川道雄、斉藤英昭編, 62-70 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 横山隆: "消化器外科周術期感染症" 炭山嘉伸編著, 227-234 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKESUE Y.ET AL: "THE EFFECTIVENESS OF PERINEAL RECTOSIGMOIDECTOMY FOR TREATMENT OF RECTAL PROLAPSE IN ELDERLY AND HIGH-RISK PATIENTS." SURG TODAY. 29(3). 290-293 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKESUE Y.ET AL: "PREDICTION FOR THE DEVELOPMENT OF POSTOPERATIVE INFECTIONS IN THE OPERATION OF ESOPHAGEAL CANCER COMPARED WITH GASTRIC SURGERY" HIROSHIMA J MED SCI.47(3). 109-113 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.ET AL: "ACUTE GASTRIC MUCOSAL LESIONS AND BACTERIAL TRANSLOCATION" THE JAPANESE JOURNAL OF ACUTE MEDICINE. 22(13). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MURAKAMI Y.ET AL: "RELATIONSHIP BETWEEN CYTOKINE AND ORGAN DYSFUNCTION UNDER VARIOUS KINDS OF SURGICAL STRESS" BIOTHERAPY. 12(5). 604-606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.ET AL: "AGE-RELATED CHANGES IN IMMUNE FUNCTIONS" AGE-RELATE GASTROENTEROLOGY(JAPANESE). 10(2). 111-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.: "POSTOPERATIVE ORGAN FAILURES DUE TO PRIMARY GASTROENTEROLOGICAL INFECTIONS YOKOYAMA T.ET AL..INFECTIOUS SHOCK" JAP J ANTIBIOT.51. 541-542 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] ASAHARA T ET AL: "LIVING RELATED PARTIAL LIVER TRANSPLANTATION FOR PRIMARY BILIARY CIRRHOSIS-A CASE REPORT." GASTROENTEROL.SURG.(JAPANESE). 21(5). 935-936 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KODAMA T,ET AL.: "POSTOPERATIVE ENTERITIS CAUSED BY METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS." HIROSHIMA J MED SCI. 47(1). 31-37 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKESUE Y.ET AL: "SURGICAL TREATMENT FOR DUODENUM INVOLVEMENT IN CROHN'S DISEASE" SURG TODAY. 27. 816-825 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] SANTO T.ET AL.: "THE EXPERIMENTAL STUDY WITH THE INDUCTION OF CYTOKINES BY METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS CAUSATIVE STRAINS OF POSTOPERATIVE ENTEROCOLITIS IN GASTROENTEROLOGICAL SURGERY" THE JAPANESE JOURNAL OF GASTROENTEROLOGICAL SURGERY. 30(7). 1734-1738 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MURAKAMI Y.ET AL.: "CORRELATION BETWEEN PERIPHERAL NEUTROPHIL,CYTOKINES,AND ORGAN FAILURES." SURG.TRAUMA.IMMUNOL.RESPONSES. 6(1). 17-19 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] TAKESUE Y.ET AL: "NEW CONCEPT OF BACTERIAL TRANSLOCATION AND ITS ROLE IN THE PATHOGENESIS OF SIRS" J.CLIN.EXP.MED.(JAPANESE). 181(1). 907-911 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.: BACTERIAL TRANSLOCATION. OGAWA M., 47-51 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.: INFECTION. OGAWA M.SAITO H., 47-51 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] YOKOYAMA T.: BACTERIAL TRANSLOCATION. SUMIYAMA Y., 227-234 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takesue Y: "The effectivencss of perineal rectosigmoidectomy for treatment of rectal prorapse in elderly and high-risk patients." Surg Today. 29・3. 290-293 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takesue Y: "Prediction for the development of postoperative infections in the opcration of esophageal cancer compared with gastric surgery." Hiroshima J Med Sci.47・3. 109-113 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "消化管不全" 救急医学. 22・13. 1954-1958 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上義昭: "各種侵襲下におけるサイトカインと臓器障害との関連" Biotherapy. 12・5. 604-606 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "加齢に伴う変化-免疫機能(-感染防御能を中心に-)" 老年消化器病. 10・2. 111-117 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "術後臓器障害の発生から見た消化器外科一次感染症" Jap J Antibiot.51. 541-542 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "感染性ショック" 消化器外科(新術前・術後管理マニュアル). 21・5. 935 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "臨床医のための救急疾患キーワード" 小川道雄編, 47-51. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "臨床侵襲学" 小川道雄、斉藤英昭編, 62-70. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 横山隆: "消化器外科周術期感染症" 炭山嘉伸編著, 227-234. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上義昭: "末梢好中球数とサイトカインおよび臓器障害との関連" 侵襲と免疫. 6・1. 17-19 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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