• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Experimental study of prevention of postoperative liver metastasis in pancreatic cancer

Research Project

Project/Area Number 09671313
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYamaguchi University

Principal Investigator

NOSHIMA Shinji (1998)  University Hospital, Yamaguchi University assistant, 医学部附属病院, 助手 (40243654)

守田 信義 (1997)  山口大学, 医学部, 助教授 (60116762)

Co-Investigator(Kenkyū-buntansha) ENOKI Tadahiko  School of Medicine, Yamaguchi University assistant, 医学部, 助手
野島 真治  山口大学, 医学部・附属病院, 助手 (40243654)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordspancreatic cancer / liver inetastasis / E-selectin / mouse / pancreatectomy / RT-PCR / tumor necrosis factor α / interleukin 1β / interleukin 6
Research Abstract

1. The changes of cytokines in the liver tissue after pancreatectomy - The effect of administration of mitomicin C into portal vein
Under intraperitoneal pentobarbital anesthesia, male balb/c mice were given distal pancreatectomy and splenectomy. The animals in the MMC group were administered mitomycin C (1mg/kg) into the portal vein soon after pancreatectomy, while the animals in the control group recieved no medication. The animals were killed 0 to 24 hours after surgery, and liver tissues were excised. After homogenization of the liver and centrifugation, tumor necrosis factor alpha, jnterleukin (IL) 1 beta , and IL 6 levels were determined by an enzyme-linked immunosorbent assay.
Tumor necrosis factor alpha and IL I beta did not change throughout the experiments in either groups ; however, IL 6 in the liver became significantly elevated from 0.680.27 and 0.43*0.20 pg/mg protein to 11.5*3.2 and 8.17*0.60 pg/mg protein, respevtively in the control and MMC groups, 30 minutes after surgery. This significant elevation of IL 6 persisted for 24 hours and 12 hours after surgery in the control and MMC groups, respectively.
2. Expression of the E-selectin (ELAM-1) gene in the liver tissue after pancreatectomy
E-selectin gene expressions in the liver were assayed by a reverse transcriptase-nested polymerase chain reaction. Expression of the E-selectin gene in the liver was not detected throughout the experimental period, while expression of the GAPDH gene was detected in every samples.
Thus, we concluded that pancreatectomy promoted IL 6 production in the liver and that rnitomycin C injection into the portal vein might reduce this elevation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi