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Significance of glutathione and bile acids metabolism for assessment of graft function after small intestinal transplantation in rats.

Research Project

Project/Area Number 09671315
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Nobuaki  Ehime University School of Medicine, First Department of Surgery, Professor, 医学部, 教授 (60135564)

Co-Investigator(Kenkyū-buntansha) FUKUHARA Toshiyuki  Ehime University School of Medicine, First Department of Surgery, Instructor, 医学部, 助手 (00228925)
YAMAMOTO Naritaka  Kyoto University, Graduate School of Medicine, Gastroenterological Surgery, Inst, 大学院医学研究科, 助手 (30253298)
KUSHIHATA Fumiki  Ehime University School of Medicine, First Department of Surgery, Instructor, 医学部附属病院, 助手 (70284396)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordssmall intestinal transplantation / bile acids metabolism / orthotopic entire intestinal transplantation / port-portal anastomosis / port-caval anastomosis / graft function / rejection / secondary bile acid / 小腸移植 / 胆汁酸分画 / サイトカイン
Research Abstract

[Aim] The aim of this research project is to study the significance of changes of bile acid component for assessment of grail function after small intestinal transplantation.
[Method] (1) Male Lewis rats, weighing 250-300g, were iso-transplanted orthotropically with entire small intestine of Lewis rats, and were aim-transplanted with small intestine of Brown-Norway rats as rejection model. Artery of the graft was anastomosed to abdominal aorta of the recipient, and portal vein of the graft was anastomosed to the portal vein of recipient(P-P), or to the inferior vena cava(P-V) in iso-graft model. In allo-graft model, P-V anastomosis was performed. Intestine was primarily anastomosed. (2) One, two and four weeks after operation, bile and blood samples were collected, and 24 kinds of bile acids were measured by HPLC.(3) The animals with rejection models were devided into 4 groups (each n=8), as follows ; Group 1 : sham operation, Group 2 : iso-graft(Lew-Lew), Group 3 : allo-graft(13N-Lew), Group 4 : FK506(O.5mg/Kg i.m.) was administrated to Group 3 every day following operation.
[Results] (l)ln iso-grafted model, bile out put did not change, bile acid concentration in bile and serum increased and showed higher level in P-V group than in P-P group. Glycine conjugates increased 2 weeks after, and normalized 4 weeks after operation. Secondary bile acids tended to increase during 2 to 4 weeks after operation. (2) ln allo-grafted(rejection) model, at the time of 1 week after operation when graft gut wall revealed moderate acute rejection histopathologically, secondary bile acid(deoxycholic acid) increased and primary bile acid(cholic acid) decreased in Group 3, and these changes were not seen in Group 4, in which FK506 was administered.
[Conclusion] The changes of increased secondary bile acid and decreased primary bile acid in serum and bile after small intestinal transplantation may inform the occurrence of rejection.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 清地 秀典 他: "小腸移植おける外来性神経再生の検討と外来性神経温存移植手技の開発" 日本消化器外科学会雑誌. 29・4. 920-925 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 清地 秀典 他: "小腸移植における外来性神経再生と保存の影響" 低温医学. 23・1. 43-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮城 和富 他: "ラット小腸移植後の胆汁酸分画についての検討" 第10回小腸移植研究会(抄録集). 15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyagi,K.et al: "Analysis of Individeeal Bile Acids in Small Bowel Transplantation in Rats." International Symposium:Growth Factors and Nutrients in Intestinal Health and Disease,Abstracts. 120 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮城 和富 他: "小腸移植における胆汁酸分画の検討" 日本移植学会雑誌(第34回総会臨時号). 33巻. 179 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 宮城 和富 他: "小腸移植における胆汁酸分画の検討" 第12回日本小腸移植研究会 抄録. 12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hidenori KIYOCHI, Atsumi ONO, Kazutomi MIYAGI, Naritaka YAMAMOTO, Katsuyuki OHNISHI, Yasuyuki SHIMAHARA, Nobuaki KOBAYASHI.: "Extrinsic Reinnervation and Extrinsic Nerve Preservation on Intestinal Transplantation in Rats." Jpn J Gastroenterol Surg. 29 (4). 920-925 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hidenori KIYOCHI, Atsumi ONO, Katsuyuki OHNISHI, Yasuyuki SHIMAHARA, Nobuaki KOBAYASHI.: "Extrinsic reinnervation and extrinsic nerve preservation after small bowel cold storaged and transplantation." Low Ternp Med. 23 (1). 43-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazutomi MIYAGI, Hidenori KIYOCHI, Yoshikazu SUZUKI, Kazuo HONDA, Nobuaki KOBAYASHI.: "Analysis Bile acid component in small Intestinal Transplantation." Japanese Journal of Transplantation 33 supplement (Proceedings of 34th annual Meeting). 113 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazutomi MIYAGI, Hidenori KIYOCHI, Yoshikazu SUZUKI, Kazuo HONDA, Nobuaki KOBAYASHI.: "Analysis of Individual Bile acid in small Bowel Transplantation" Japanese Journal of Surgery Metabolism and Nutrition. 32 (3). 147 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazutomi MIYAGI, Hidenori KIYOCHI, Yoshikazu SUZUKI, Kazuo HONDA, Nobuaki KOBAYASHI.: "Analysis of Individeeal Bile Acids in Small Bowel Transplantation in Rats." International Symposium : Growth Factors and Nutrients in Intestinal Health and Disease, Abstracts. 120 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyagi, K. et al: "Analysis of Individeeal Bile Acids in Small Bowel Transplantation in Rats." International Symposium:Growth Factors and Nutrients in Intestinal Health and Disease, Abstracts. 120 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宮城 和富 他: "小腸移植における胆汁酸分画の検討" 外科と代謝・栄養. 32巻3号. 147 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宮城 和富 他: "小腸移植における胆汁酸分画の検討" 日本移植学会雑誌(第34回総会臨時号). 33巻. 179 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宮城 和富 他: "小腸移植における胆汁酸分画の検討" 第12回日本小腸移植研究会 抄録. 12 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 清池 秀典 他: "小腸移植おける外来性神経再生の検討と外来性神経温存移植手技の開発" 日本消化器外科学会雑誌. 29・4. 920-925 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 清池 秀典 他: "小腸移植における外来性神経再生と保存の影響" 低温医学. 23・1. 43-51 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宮城 和富 他: "ラット小腸移植後の胆汁酸分画についての検討" 第13回西日本臨床胆汁酸研究会(抄録集). 13- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宮城 和富 他: "ラット小腸移植後の胆汁酸分画についての検討" 第10回小腸移植研究会(抄録集). 15- (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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