| Project/Area Number |
09671316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Ehime University |
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Principal Investigator |
KUSHIHATA Fumiki Ehime University, Department of Surgery, Assistant Professor, 医学部附属病院, 助手 (70284396)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Seiji Ehime University, Department of 1st.Anatomy, Professor, 医学部, 教授 (40173843)
KOBAYASHI Nobuaki Ehime University, Department of Surgery, Professor, 医学部, 教授 (60135564)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | basic fibroblast growth factor (bFGF) / liver surgery / growth factor / immunohistochemistry / hepatocyte regeneration / intranuclear bFGF / cell proliferation / FGF receptor |
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| Research Abstract |
This project was performed to investigate the function of intranuclear bFGF in regenerating hepatocyte and its crinical application in liver surgery.
Regenerating hepatocytes with bFGF
Immunoblot experiments and ELISA showed a transient increase in high-molecular-weight forms of bFGF in the nuclear subcellular fraction of regenerating liver 4Th after partial hepatectomy. By light microscopy, bFGF and PCNA immunoreactivities were detected in the nuclei of hepatectomy. Electron microscopy demonstrated bFGF-like immunoreactivity mainly in the nuclear euchromatin and rarely in the heterochromatin or nucleoli of regenerating hepatocytes. These results suggest an important role of the high-molecular-weight forms of bFGF in hepatocyte proliferation and mitosis.
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