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Establisment of tumor vaccine using autologous dendritic cells and its application for addaptive immunotherapy

Research Project

Project/Area Number 09671321
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MORISAKI Takashi  School of Medicine, Kyushu University, 1st Dep.Surgery,, 医学部, 助手 (90291517)

Co-Investigator(Kenkyū-buntansha) KOJIMA Masayuki  School of Medicine, Kyushu University, 1st Dep.Surgery,, 医学部, 医員
UCHIYAMA Akihiko  School of Medicine, Kyushu University, 1st Dep.Surgery,, 医学部, 助手 (20294936)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsdendritic cells / tumor vaccine / colon cancer / 癌特異的免疫療法
Research Abstract

Dendritic cells (DC) possess the most potent antigen-prersenting activity among various antigen presenting cells. This study aims preclinical application of tumor vaccine using autologous dendritic cells. We tried to establish autologous dendritic cell vaccine using autologous colon cancer cells and dendritic cells, and focused on investigation of the methods for effective induction of autolpogpus tumor spesific T cells and culture system for tumor-spesific dendritic cells. We first established colon carcinoma cell line, CE-1, from the 38 years. male patient with advanced colon cancer. These tumor cells revealed HLA-type Class I A31, and A11. We tried to establish tumor-spesific adendritic cells using autologouis cells and HLA-matched healthy volunteer. 1-2 x 10^6 dendritic cells were obtained from 100 ml of heparinized peripheral blood and culture media containing IL-4 and GM-CSF.When irradiated or freezing-thawing tumor cells were added to the dendritic cells and furtherly cultured in the presence of IL-4 and GM-CS F, tumor-spesific dendritic cells were obtained. These cells were positive for expression of IL-12 and IFN-gamma mRNA and the expression of HLA-DR, CD80, and ICAM-1.
When autologous T cells were added to the tumor-pulsed dendritic cells and cultured in the presence of IL-2 and IL-4 for subsequent 2 weeks, autologous tumor-reactive CTL were obtained. Because theses T cell possess cytotoxic activity against autologous tumor cells releasing IFN-gamma. However we failed in culture of dendritic cells in a large number using gellatin-beads, which was one of aims of this study. Further studies are needed to establish and verify effective systems for culture of autologpus-tumor pulsed dendritic cell vaccine.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

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