| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
i. Induction of smooth muscle cell in the capsule of human primary hepatooellular carcinoma : Vimentin, smooth muscle cell actin (1A4, HHF35 and CGA7), myosin heavy chain isoform (SMemb, SM1, SM2) were studied immunohistochemically in the surgical specimen of human liver cirrhosis and cancer. In the liver cirrhosis, Vimentin, 1A4 and Smemb were positive, but HHF35 and CGA7 were not. On the other hand, spindle cells in the capsule of primary liver cancer expressed above markers except SM2. We conclude that differentiated smooth muscle cells are induced in the fibrous capsule of primary liver cancer.
ii. Expression of VEGF and its receptors in the chronic liver disease : We examined VEGF and its receptors (Flt-1 and KDR/Flk-1) in the frozen section of bicpsied human liver tissue. In the normal liver, small number of Kupffer cells expressed VEGF but not its receptors. In the congested liver, many Kupffer cells and endothelial cells of arterioles expressed VEGF but not its receptors. In the
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chronic hepatitis and liver cirrhosis, the expression of VEGF was enhanced in the area infiltrated with inflamatory cells. The receptors of VEGF were expressed in the infiltrated macrophages and the endothelial cells of neovasculature. The distribution of VEGF was similar to it of VEGF receptors. This result indicates that VEGF system plays an important role in the angiogenesis of chronic liver disease, ie, congested liver, chronic hepatitis and liver cirrhosis.
iii. Expression of VEGF and its receptors in the metastatic liver cancer originated from colo-rectal cancer. We examined the expression of VEGF and its receptors in the frozen surgical specimen of liver metastasis from colon cancer immunohistochemically. In non-cancerous part, small number of Kupffer cells expressed VEGF but not its receptors. Within the fibrous part at the rim of the cancer nest, small number of macrophages expressed VEGF but not its receptors. On the other hand, the liver tissue just around the cancer, VEGF and its receptors were strongly expressed in the accumulated macrophages and the endothelial cells of neovasculature. VEGF system has a strong correlation with the development of the liver metastasis of colo-rectal cancer. Less
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