| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
Calcification is the most important mode of failure in biomaterials used for cardiovascular surgery. It is already known that epoxy compound fixation decrease the incidence of calcification. However, epoxy fixation requires long duration to get enough crosslinking density. Therefore the biomaterials to be fixed should be xenografts or allografts. In this study, we used pericardial autograft in view of rejection. But the technique requires that fixation finishes in short duration, that is, during operation time. So, we developed a new fixation technique with epoxy compound to fix pericardial autograft. The improvements over conventional epoxy fixation are, dose up in epoxy compound, use of catalyst, and increase in reaction temperature. This study was undertaken to investigate the calcification and the biocompatibility of pericardial autograft fixed by our new technique (group I) in vivo. Non treated fresh pericardial autograft (group II) and glutaraldehyde fixed bovine pericardium (groupIII) were used as controles. Significant decrease of calcification was found by our new technique rather than by both non treated and glutaraldehydefixed bovine pericardium : 0.245*0.140 (group I), 0.706*0.249 (group II), 0.869*0.111 (group III), mug(Ca)/mg(dry weight) after two months pulmonary artery wall implantation in dogs. The historical observation of those three groups revealed that pericardial autograft fixed by our technique with epoxycompound has good biocompatibility.
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