| Project/Area Number |
09671375
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
FUJINO Shozo Shiga Univ.of Med.Sci., 2nd Dep.of Surg., Assist.Prof., 医学部, 講師 (10209075)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Atsumi Shiga Univ.of Med.Sci., 2nd Dep.of Surg., Professor, 医学部, 教授 (80026971)
INOUE Shuhei Shiga Univ.of Med.Sci., 2nd Dep.of Surg., Assist.Prof., 医学部, 助手 (30273402)
TEZUKA Noriaki Shiga Univ.of Med.Sci., 2nd Dep.of Surg., Assist.Prof., 医学部, 助手 (40303771)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Animal lung / Artificial lung / Nitroprusside / Lung transplantation |
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| Research Abstract |
The following studies were carried out for the purpose of developing an artificial lung from animal lung which has more durability and less antigenicity.
1. Evaluation of gas exchange ability of fixed lung using a perfusion system with mechanicaldeoxygenator : An apparatus to preserve the resected lung by continuous perfusion with deoxygenated blood was developed. Free rabbit lungs which were flushed out with nitroprusside and fixed by the mixture of paraformaldehyde and glutaraldehyde (4% paraform-aldehyde + 0.325% glutaraldehyde) were evaluated its gas exchange ability using this system. Four of 8 lungs were able to evaluate during 5 hour assessment period. Mean D-PO2 (the difference of in- and out-flowing blood ) were 55 ton at 5 hour of assessment (FiO2 = 0.4).
2. Evaluation of gas exchange ability of fixed lung using an in vivo perfusion model : The left whole lung of canine was flushed out with nitroprusside and fixed by the same fixative in site. Gas exchange ability was evaluated under bilateral ventilation (Group I) and left single lungventilation (Group II). In Group I (n = 5), two dogs were died at 2 and 3 hours of assessmsnt respectively. D-P02 (the difference of arterial and venous blood) were 145, 180 and 260 ton after 5-hour assessment in three dogs (FiO2 = 1.0).While, in Group II (n = 5), 3 of 5 dogs were died just after ligation of right lung, and other two dogs could be alive for 2 and 3 hours. D-PO2 at the first assessment was 142 and 205 torr
. These findings suggest that the resected lung retains gas exchange ability comparable to that of the fresh lung if it has been fixed under appropriate conditions, but it is very difficult to flush out fixative from lung completely. In this study, nitric oxide donor Nitroprusside could improve flushing out, but further improvements in the method for fixation of the lung are desired.
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