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Attempt at creating donor pig by nuclear transplantation for xenotrasplantation

Research Project

Project/Area Number 09671389
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionNara Medical University

Principal Investigator

TANIGUCHI Shigeki  Nara Medical university, Department of Medicine, Professor, 医学部, 教授 (90183467)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsheart taransplantation / xenotrasplantion / gene transfer / gene gun / a 1-3 galactosiltransferase knockout / EB-Virus episomal vector / cryopreserved xenograft transplantation / 凍結保存異種組織移植 / α 1-3 galactosyltransferase knock out
Research Abstract

1) My colleges and I have attempted to create alpha-gal knockout pig. Now, we are not successful in it yet. 2) To examine the means to change the allo- or xeno-reactivity after transplantation, we pefformed ex vivo transfer of marker gene to heart grafts using adenovirus vector. Successful gene transfer and expression of beta-gal gene were demonstrated. This result demonstrated that cardiomyocytes of the grafts expressed an exogenous gene products by adenovirus mediated gene transfer under hypothermic preservation condition. 3) We found that gene gun- mediated transfer of the EBV-based episomal vector into rat hearts results in long-lasting expression of a marker gene. Combined use of the gene gun and EBV-based episomal vector may contribute to gene therapy of cardiovascular diseases. To our knowledge, this is the first study showing that in vivo gene transfer into heart can be achieved using a gene gun. 4) We transfected rodent cells with EBV-episomal vectors carrying E.coli b-galactosidase gene as a marker gene. Significantly higher transient expression was demonstrated, both in vivo and in vitro, in rodent cells transfected with the EBV vector compared to the cells transfected with a conventional vector. 5) We transplanted porcine cryopreserved aortic valve to dog aorta for the purpose of assessment of the ability of discordant xeno-tissue-transplantation. Compared with fresh tissue, cryopreserved valves kept cell viability. This result suggests the ability of clinical use.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Satosi Gojo: "Gene transfer into the donor heart during cold preservation for heart transplantation." Ann.Thorac.Surg.65. 647-652 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomiyasu K, et al: "Gene transfer in vitro and in vivo with Epstein-Barr virus-based episomal vector results in markedly high transient expression in rodent cells." Biochem Biophys Res Commun. 30. 733-738 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoshi Gojo, kazuo Niwaya, Shigeki Taniguchi, Kazuhiko Nishizaki, Soichiro Kitamura: "Gene transfer into the donor heart during cold preservation for heart transplantation." Ann Thorac Surg. 65(3). 647-52 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomiyasu K, Satoh E, Oda Y, Nishizaki K, Kondo M, Imanishi J, Mazda O: "Gene transfer in vitro and in vivo with Epstein-Barr virus-based episomal vector results in markedly high transient expression in rodent cells." Biochem Biophys Res Commun.253 : 3. 733-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomiyasu K,et al: "Gene transfer in vitro and in vivo with Epstein-Barr virus-based episomal vector results in markedly high transient expression in rodent cells." Biochem Biophys Res Commun. 30. 733-738 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Satosi Gojo: "Gene transfer into the donor heart during cold preservation for heart transplantation." Ann.Thorac.Surg.65(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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