| Project/Area Number |
09671395
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
MIZUNO Asatoshi Jikei University School of Medicine, Lecturer, 心臓外科, 講師 (60174033)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | nitric oxide / L-arginine / endotbelium / 内皮細胞 |
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| Research Abstract |
Early death may follow technically successful operation in patients with good ventricular performance. The causes of cardiac depression are complex and sometimes may involve injury of vascular eudothelium. Endotbelial damage may also cause myocyte dysfunction because of imbalance between vasodilalor (such as nitric oxidc ; NO) and vasoconstrictor substances. NO is produced from the amino add L-arginine that combines with molecular oxygen and produces citnillin via constitutive NO synthase. If ischemia-reperfusion injury causes endothelial dysfunction, subsequent release of NO decreases, vasorelaxation is altered, platelet aggregation is increase, and leukocyic adherence to vascular endothelium is enhanced with a comcomitant increase in cytotoxic ocygen radical production.
This study tested the following ; 1)whether endotbelial dysfunction (reduced nitric oxide release) exists despite good contractile performance, 2)whether supplementation of blood canlioplegic solution with nitric oxide precursor L-arginine auguments nitric oxide and restores endothelial function, 3) what is optimal dose of L-arginine supplemented in cardioplegic solution.
Results ; Complete systolic recovery followed infusion of blood cardioplegic solution and of blood cardioplegic solution plus L-arginine(2 mmol/L). Conversely, contractility recovered approximately 70% after infusion of blood cardioplegic solution plus high dose L-arginine(10 mmol/L). Postischemic nitric oxide production fell in the group that received blood cardioplegic solution. The discrepancy between contractile recovery and endothelial dysfunction can be reversed by adding L-arginine to blood cardiolegic solution.
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