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INTIMAL HYPERPLASIA and GENE MAPPING IN MOYAMOYA DISEASE

Research Project

Project/Area Number 09671412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE

Principal Investigator

MATSUSHIMA Yoshiharu  TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (20134679)

Co-Investigator(Kenkyū-buntansha) TANAKA Masashi  TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (30301154)
YAMAMOTO Kiyotaka  TOKYO METROPORITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF CELL BIOLOGY,CHIEF RES, 細胞生物部門, 主任研究員 (90073022)
AOYAGI Masaru  TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,LECTURER, 医学部, 講師 (40134704)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsmoyamoya disease / familial occurence / gene analysis / muscle, smooth / arterial wall / elastin / IL-1
Research Abstract

(1) We investigated the elastin synthesis and elastin mRNA levels both in sections from superficial temporal arteries (STA) and cultured SMCs derived from moyamoya patients. The intimal lesions of STA of moyamoya patients contained strongly stained multilayered elastic fibers while no difference in collagen staining was seen between moyamoya and control STA.In situ hybridyzation for tropoelastin transcripts showed strong positive signals in neontimal SMCs of moyamoya patients. Elastin mRNA and protein synthesis levels were significantly greater in cultured moyamoya SMCs than control SMCs. The data indicate that the increased elastin synthesis and mRNA is a stable maker of moyamoya SMCs and that genetic changes which can regulate the elastin transcripts may be responsible for the pathogenesis of moyamoya disease. (2) We examined the chemotactic and proliferative activities of growth factors and inflammmatroy cytokines on arterial smooth muscle cells (SMC) derived from moyamoya patients. Interleukin-1beta (IL-1beta) significantly stimulated the migation and DNA syntehsis of control SMC, while it inhibited moyamoya SMC migration. The differences in responses to PDGF and IL-1 in moyamoya SMC are involved in the mechanism by which intimal thickening develops in moyamoya disease. (3) We investigated the familial occurence of moyamoya disease using magnetic resonance angiography (MRA). We colleted three familial moyamoya pedigrees. We collected blood samples from moyamoya patients and normal subjects in these families, and are now performing linkage analysis using the genomic DNA.The final results have not been obtained. However, much larger pedigrees may be necessary for the sucessful gene mapping of this disease.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Yamamoto M,Aoyagi M,Matsushima Y et al.: "Increase in elastin gene expression and protein systhesis in arterial smooth muscle cells derived from patients with moyamoya disease." Stroke. 28. 1733-1738 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M et al.: "Expression of p53 protein and p53 gene transcripts in rabbit carotid arteries after balloon denudation." Histochem Cell Biol. 107. 365-370 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松島善治、青柳 傑 他: "小児期EDAS施行後の成人期脳血管撮影所見について" 小児の脳神経. 22. 206-209 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松島善治: "総説-モヤモヤ病に対する間接的血管吻合術" 脳神経外科. 26. 769-786 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto M,Aoyagi M,et al.: "Differences in cellular responses to mitogens in arterial smooth muscle cells derived from patients with moyamoya disease." Stroke. 29. 1188-1193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,et al.: "Immunolocalization of matrix metalloproteinases in rabbit carotid arteries after balloon denudation." Histochem Cell Biol. 109. 97-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松島善治、山浦晶、森竹浩三編: "脳血管障害;モヤモヤ病-EDASを中心として、小児脳神経の外科-Standard and Modified Techniques-" 医学書院、東京, 329-334 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushima Y: "Moyamoya Disease,Chapter 48 of “Principle and Practice of Pediatric Neurosurgery"" eds A.Leland Albright,Ian F.Pollack,P.David Adelson,Thieme,New York, 1053-1069 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto M,Aoyagi M,Fukai N,Matsushima Y,Yamamoto K.: "Differences in cellular responses to mitogens in arterial smooth muscle cells derived from patients with moyamoya disease." Stroke. 29. 1188-1193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyagi M, Yamamoto M,Azuma H,Nagashima G,Tamaki M,Niimi Y,Hirakawa K,Yamamoto K: "Immunolocalization of matrix metalloproteinases in rabbit carotid arteries after balloon denudation." Histochemistry. 109. 97-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto M,Aoyagi M,Tajima S,Wachi H,Fukai N,Matsushima Y,Yamamoto K.: "Increase in elastin gene expression and protein synthesis in arterial smooth muscle cells derived from patients with moyamoya disease." Stroke. 28. 1733-1738 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Nagashima G,Niimi Y,Hirakawa K,Yamamoto K.: "Expression of p53 protein and p53 gene transcripts in rabbit carotid arteries after balloon denudation." Histochemistry. 107. 365-370 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Niimi Y,Tajima S, Hirakawa K,Yamamoto K.: "Smooth muscle cell proliferation, elastin formation, and tropoelastin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denudation." Histochemistry. 107. 11-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto M,Aoyagi M,Azuma H,Yamamoto K.: "Changes in osteopontin mRNA expression during phenotypic transition of rabbit arterial smooth muscle cells." Histochemistry. 107. 279-287 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushima Y,eds A.Leland Albright, Ian F.Pollack, P.David Adelson: Moyamoya Disease, , Chapter 48 of "Principle and Practice of Pediatric Neurosurgery". THeme, New York, 1053-1069 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 松島善治、青柳 傑 他: "小児期EDAS施行後の成人期脳血管撮影所見について" 小児の脳神経. 22. 206-209 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松島善治: "総説-モヤモヤ病に対する間接的血管吻合術" 脳神経外科. 26. 769-786 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nariai T, Matsushima Y et al.: "Post-hyperventilatory steal response in chronic cerebral hemodynamic stress. A Positron Emission Tomography study." Stroke. 29. 1281-1292 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto M, Aoyagi M, et al.: "Differences in cellular responses to mitogens in arterial smooth muscle cells derived from patients with moyamoya disease." Stroke. 29. 1188-1193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Aoyagi M, Yamamoto M, et al.: "Immunolocalization of matrix metalloproteinases in rabbit carotid arteries after balloon denudation." Histochem Cell Biol. 109. 97-102 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto M, Aoyagi M, et al.: "Decrease in p53 protein in cultured cardinal ligament fibroblasts from patinets with uterine prolapsus uteri." Cell Biology Int. 22. 31-40 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松島善治、山浦晶、森竹浩三編: "脳血管障害;モヤモヤ病-EDASを中心として、小児脳神経の外科-Standard and Modified Techniques-" 医学書院、東京, 329-334 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsushima Y: "Moyamoya Disease, , Chapter 48 of “Principle and Practice of Pediatric Neurosurgery"" eds A. Leland Albright, Ian F. Pollack, P. David Adelson, Thieme, New York, 1053-1069 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsushima Y, Oian L, Aoyagi M: "Comparison of Moyamoya disease in Japan and Moyamoya disease(or syndrome)in People's Republic of China." Clinical Neurology and Neurosurgery. 99,Suppl.2. S19-S22 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushima Y, Aoyagi M, Nariai T, Takada Y, and Hirakawa K: "Long-term intelligence outcome of post-EDAS childhood Moyamoya patients." Clinical Neurology and Neurosurgery. 99,Suppl.2. S147-S150 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyagi M, Fukai N, Yamamoto M, Mastushima Y, Yamamoto K: "Development of Intimal thickening in STA in patients with Moyamoya disease" Clinical Neurology and Neurosurgery. 99,Suppl.2. S213-217 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyagi M, Yamamoto M, Azuma H, Niimi Y, Tajim S, Yamamoto K: "Smooth musble cell proliferation, elastin formation,and tropoelastin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denudation" Histochem Cell Biol. 107. 11-18 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ymanoto M, Aoyagi M, Azuma H, Yamamoto K: "Changes in ostcopontin mRNA expression during phenotypic transition of rabbit arterial smooth muscle" Histochem Cell Biol. 107. 279-287 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyagi M, Yamamoto M, Azuma H, Nagashima G, Niimi Y, Yamamoto K: "Expression of p53 protein and p53 gene transcript in rabbit carotid arteries after baloon denudation" Histochem Cell Biol. 107. 365-370 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wakomoto H, Yoshida Y, Aoyagi M Hirakawa K, Hamada H: "Efficient retrovirus-medlated cytokine-gene transduction of primary-cultured human giioma cells for tumor vaccination therapy" Jpn J Cancer Res. 88. 296-305 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Funato H, Inoshita N, Okada F, Yamamoto S, Aoyagi M: "Cystic ganglloneurocytoma outside ventricular region" Acta Neuropathol. 94. 95-98 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tamaki M, Ohno K, Niimi Y, Aoyagi M, Nagashima G, Ichimura K, Hirakawa K: "Parenchymal damage in the territory of the anterior choroidal artery following supraophthalmic intracarotid administration of CDDP for treatment of malignant glioma" J Neuro-Oncol. 35. 65-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto S, Wakimoto H, Aoyagi M, Hirakawa K, Hamada H: "Modulation of motility and proliferation of glioma cells by hepatocyte growth factor" Jpn J Cancer Res. 88. 564-577 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyagi M, Yamamoto M, Azuma H, Nagashima G, Tamaki M, NIimi Y, Hirakawa K, Yamamoto K: "Immmnolocalization of matrix metalloproteinases in rabit carotid after baloon denudation" Histochem and Cell Biol. 109. 97-102 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hara M, Aoyagi M, Nagashima G, Wakimoto H, Mikami T, Yamamoto S, Tamaki M, Hirakawa K: "Recurrence in meningeal hemangioglastomas" Surg Neurol. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto K, Yamamoto M, Akzawa K, Tajima S, Wakimoto K, Aoyagi M: "Decrease in elastin gene expression and protein synthesis in fibroblasts derived from cardinal ligaments of patients with prolapse uteri" Cell Biology International. (in press). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto M, Aoyagi M, Akazawa K, Tajima S, Yamamkto K: "Decrease in p53 Protein in cultured cardinal llgament fibroblasts from patients with uterine prolapse" Cell Biology International. (in press). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushima Y: "Chapter of Moyamaya disease in Principles and Practice of Pediatric Neurosurgery(ed.Albright AL et al.)" Thieme (in press), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushima Y: "Chapter of Moyamoya disease in Atlas of Pediatric Neurosurgery(ed.Albright AL et al.)" Thieme (in press), (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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