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Telomerase activity in brain tumors.

Research Project

Project/Area Number 09671415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionGIFU UNIVERSITY

Principal Investigator

SAKAI Noboru  Gifu University, School of Medicine, Professor, 医学部, 教授 (10021487)

Co-Investigator(Kenkyū-buntansha) YOSHIMI Naoki  Gifu University, School of Medicine, Assistant Professor, 医学部, 助教授 (30166996)
IWAMA Toru  Gifu University, School of Medicine, Research Associate, 医学部附属病院, 助手 (20303498)
竹中 勝信  岐阜大学, 医学部, 助手 (00283292)
上田 竜也  岐阜大学, 医学部・附属病院, 助手 (60283305)
篠田 淳  岐阜大学, 医学部・附属病院, 講師 (50273131)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTelomerase / telomerase activity / brain tumor / TRAP assay / malignancy / differentiation / グリオーマ / テロメラーゼ / 文化誘導 / 増殖能 / 培養細胞 / cAMP / 乏突起膠腫
Research Abstract

Telomerase activity in brain tumors was analyzed. Telomerase activity was examined in surgically resected 41 brain tumors, A172 human glioblastoma cells, and C6 rat glioma cells by means of telomeric repeat amplification protocol (TRAP) assay. In the 41 tumors, the activity of telomerase was compared with histologic diagnosis, the MIB-1 proliferative cell index and patient's prognosis. In A 172 glioblastoma cells and C6 glioma cells, the changes in the telomerase activity of the cells were analyzed during differentiation induced by dibutyryl cyclic AMP, theophylline and retinoic acid. Telomerase activity in rat gliomas induced by ethylnitrosourea administration in pregnant rats was also examined.
Twelve of 20 glioblastomas, 2 of 2 oligodendrogliomas and 3 of 3 metastatic brain tumors demonstrated telomerase activity. However, anaplastic astrocytoma, low grade astrocytoma and meningioma exhibited no activity. Although no clear relations were confirmed in glioblastoma between telomerase activity and the MIB-1 proliferative cell index, the telomerase activity tended to correlate with patient's prognosis. After the cell differentiation, the telomerase activities of A 172 glioblastoma cells and C6 glioma cells were strongly decreased. There were no different telomerase activities among growth stages of rat gliomas induced by ethylnitrosourea.
These results suggest that telomerase activity may be an important marker of brain tumor malignancy and that functional inhibition of the activity may play a role during differentiation of glioma cells.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] K Nakatani, et al: "The significant role of telemerase activity in human brain tumors"Cancer. 80. 471-476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J Shinoda, et al: "Clinical trial of external beam-radiotherapy combined with daily administration of low-dose cisplatin for supratentorial glioblastoma multiforme -A pilot study"J Neuro-Oncology. 35. 73-80 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, et al: "Alteration of telo, erase activity during differentiation of A172 human glioblastoma cells"Proceeding of 11th International Congress of Neurological Surgery. 1739-1743 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sakai, et al: "Molecular cloning of a cDNA encoding a serine protease homologous to complement C1s precursor from rat C6 glial cells and its expression during glial differentiation"Gene. 209. 87-94 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, et al: "The significance of the expression of tumor suppressor gene DCC in human gliomas"J Neuro-Oncology. 40. 237-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Okumura, et al: "The characterization of human brain tumor using magnetization transfer technique in magnetic resonance imaging"Neuro Res. 21. 250-254 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sakai, S Nakashima, S Yoshimura, K Nakatani, K Takenaka, N Sakai, Y Nozawa: "Expression of CD44 variant isofomes and their relationship to cellular differentiation in human gliomas."Clinical Neurology and Neurosurgery. 99:Supple 1. s127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, H Sakai, K Takenaka, J Shinoda, N Sakai: "Alteration of telomerase activity during differentiation of A172 human glioblastoma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S Yoshimura, H Sakai, K Takenaka, K Nakatani, H Yamakawa, N Sakai, S Nakashima, Y Nozawa: "Intracellular signal transduction during ceramide-induced apoptosis in cultured glioma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S Yoshimura, H Sakai, K Ohguchi, S Nakashima, Y Banno, Y Nishimura, N Sakai, Y Nozawa: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat c6 glial cells."Journal of Neurochemistry. 69. 713-720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, J Shinoda, N Sakai: "The significant role of telomerase activity in human brain tumors."Cancer. 80. 471-476 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J Shinoda, N Sakai, A Hara, T Ueda, H Sakai, K Nakatani: "Clinical trial of external beam-radiotherapy combined with daily administration of low-dose cisplatin for supratentorial glioblastoma multiforme -A pilot study."Journal of Neuro-Oncology. 35. 73-80 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, H Sakai, K Takenaka, J Shinoda, N Sakai: "Alteration of telo,erase activity during differentiation of A172 human glioblastoma cells."Proceeding of 11th International Congress of Neurological Surgery. 1739-1743 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S Yoshimura, H Sakai, K Takenaka, K Nakatani, H Yamakawa, N Sakai, S Nakashima, Y Nozawa: "Intracellular signal transduction during ceramide-induced apoptosis in cultured glioma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Sakai, S Nakashima, S Yoshimura, Y Nishimura, N Sakai, Y Nozawa: "Molecular cloning of a cDNA encoding a serine protease homologous to complement Cls precursor from rat C6 glial cells and its expression during glial differentiation."Gene. 209. 87-94 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S Murase, M Saio, K Takenaka, J Shinoda, Y Nishimura, N Sakai, T Takami: "Increased levels of CSF soluble CD27 in patients with primary central nervous system lymphoma."Cancer Letters. 132. 181-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Nakatani, N Yoshimi, H Mori, H Sakai, J Shinoda, T Andoh, N Saka: "The significance of the expression of tumor suppressor gene DCC in human gliomas."J Neuro-Oncology. 40. 237-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A Okumura, K Takenaka, Y Nishimura, Y Asano, N Sakai, K Kuwata, S Era: "The characterization of human brain tumor using magnetization transfer technique in magnetic resonance imaging."Neurol Res. 21. 250-254 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A. Okumura et al.: "The characterization of human brain tumor using magnetization transfer technique in magnetic resonance imaging."Neurol Res. 21. 250-254 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S Yoshimura,et al: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat c6 glial cells." J Neurochem. 69. 713-720 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K Nakatani,et al: "The significant role of telomerase activity in human brain tumors." Cancer. 80. 471-476 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] N Sakai,et al: "Surgical treatment of cavernous angioma involving the brainstem." Proceeding of 11th International Congress of Neurological Surgery. 1159-1163 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] S Yoshimura,et al: "Intracellular signal transduction during ceramide-induced apoptosis in cultured gilioma cells." Clinical Neurology and Neurosurgery. 99 (suppl). s128 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] H Sakai,et al: "Messenger RNA fingerprinting analysis using arbitrarily primed PCR (RAP) of genes expressed during rat C6 glioma cell differentiation." Brain Tumor Pathology. 14. 119-123 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] H Sakai,et al: "Molecular cloning of a cDNA encoding a serine protease homologous to complemnet Cls precursor from rat C6 glial cells and its expression during glial differentiation." Gene. 209. 87-904 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 坂井 昇他: "グリオーマ細胞の分化誘導の試み" 脳外. 25. 875-882 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K Nakatani, et al: "The significant role of telomerase activity in human brain tumors." Cancer. 80. 471-476 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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