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A role of the K-channel in regulation of cerebral microcirculation

Research Project

Project/Area Number 09671418
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionNagoya University

Principal Investigator

TAKAYASU Masakazu  School of Medicine, Nagoya University Assistant Prof., 医学部, 助手 (60216794)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsK-channel / Cerebral microcirculation / arterioles / ポタシュウムチャンネル
Research Abstract

Although the K-channel is a well-known regulator of the circulation in many organs such as heart and lung, little is known about the K-channel in cerebral microcirculation. We investigated the role of K-channel in regulation of cerebral microcirculation by meauring vessel diameter and the membrane potential simultaneously in isolated cerebral arterioles. (Methods) Penetrating intracerebral arterioles, about 50 mu m in diameter, were isolated surgically from the middle cerebral arteries of male Sprague-Dawley rats, and were cannulated under the inverted microscope. After transmural pressure was set to 60 mmHg and bath temperature was brought to 37.5゚C, the vessels developed spontaneous tone, contracting to approximately 70% of theri maximum passive diameters. The vessel diameter and the membrane potential of the smooth muscle cells were monitored simultaneously. The membrane potential was measured through the glass electrode filled with potassium acetate, which was penetrated into the cells using an electric pulse manipulator. (Results) When glibenclamide (10 mu M), an inhibitor of ATP-sensitive K-channels, was administered, the vessel diamter decreased by 5.1*4.4% from a diameter of 59.0*13.1 mu m (n= 10) and membrane potential was depolarozed form -33*4.2 mV to -29.7*3.6 mV.On the other hand, the membrane potential did not change at all when nitric oxide synthase inhibitor (NG-monomethyl-L-arginine : 100 mu M) was administered. (Conclusion) The present study suggests that K-channel plays an important role in maintenance of basic tone of arterioles in the cerebral microcirculation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Mori M,Takayasu M,Suzuki Y,Shibuya M,Yoshida J,Hidaka H: "Vasodilatory effects of C-type natriuretic peptide on cerebral parenchymal arterioles in rats." Eur J Pharmacol. 320. 183-186 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori M,Takayasu M,Suzuki Y,Shibuya M,Yoshida J,Hidaka H: "Effects of adrenomedullin on rat cerebral parenchymal arterioles." Eur J Pharmacol. 330. 195-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kajita Y,Takayasu M,Dietrich HH,Dacey RG,Jr: "Possible role of vasodilatory propagation in the regulation of rat cerebral microcirculation." Microcirculation annual. 13. 81-84 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kajita Y,Takayasu M,Dietrich HH,Dacey RG,Jr: "Vasodilatory effect of basic fibroblast growth factor in isolated rat intracerebral arterioles." Microcirculation annual. 14. 81-82 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kajita Y,Takayasu M,Dietrich HH,Dacey RG,Jr: "Possible role of nitric oxide in autoregulatory response in rat intracerebral arterioles." Neurosurgery. 42. 834-842 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary

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Published: 1997-04-01   Modified: 2016-04-21  

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