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Studies on invasive properties of malignant gliomas and development of treatment modalities for these tumors.

Research Project

Project/Area Number 09671451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University

Principal Investigator

NAGASHIMA Tadashi  Teikyo University, School of Medicine, Professor, 医学部, 教授 (70217991)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Mineko  Teikyo University, School of Medicine, Assistant Professor, 医学部, 講師 (90229972)
MATSUNI Akira  Teikyo University, School of Medicine, Associate Professor, 医学部, 助教授 (00242058)
平川 誠  帝京大学, 医学部, 助手 (30218771)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsinvasion / glioma / in vivo / nude rat / 蛍光標識 / 浸潤能 / 免疫組織化学
Research Abstract

5X10ィイD15ィエD1 fast blue-labeled U87MG or U251MG cells in 10μl of DMEM were injected into the right basal ganglia of 9-10weeks nude rat ( F344 / Njcl-rnu ). Two weeks after tumor implantation, the rat brains were fixed with 2% paraformaldehyde followed by 30% sucrose, embedded in OCT compound, freezing for cutting into 10μm thick sections with a cryostat. Sections were stained with hematoxylin and eosin, and observed under fluorescent microscope.
The tumor cells invaded into the surrounding normal brain tissue and the scattered fast blue labeled-cells were observed around the solid tumor mass in U251MG tumor model, whereas the tumor cells were localized and the fast blue labeled- cells were not observed around the solid tumor mass in U87MG tumor model. The labeled-tumor cells which invaded into the narrow space of lateral ventricle were clearly distinguished in some cases. These results revealed that the brain tumor model using nude rat is useful to investigate the invasive properties of human glial tumors.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 長島正 他: "グリオーマの成長・栄養因子"脳と神経. 49. 214-226 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田中聡,長島正 他: "悪性グリオーマ補助療法におけるGCSFの有用性"脳神経外科ジャーナル. 6. 827-832 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka, S, Nagashima T, et al: "In vitro inhibition of binding of tumor necrosis factor (TNF)-2 by monoclonal untibody to INF receptor of glioma celland monocyte"Neurologia medico-chirurgica. 38. 812-818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 村上峰子 他: "脳腫瘍の頭痛"診断と治療. 86. 905-908 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuno A et al: "An improved ultrastractural double-staining method for rat grouth hormone and its mRNA using white resin"H : stochemical Journal. 30. 105-109 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuno A et al: "Application of ultrastructural in situ hybridigation combined with *mmunoh : stchemistry to pathophysiological studies"Acta Histochem. Cytochem. 31. 259-265 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 長島正 他(分担): "Amual Review 神経 1998"中外医学社. 324 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 長島正 (分担): "前頭葉・登頂葉 〔機能 ・ 解剖 ・ 手術〕"メジカル ビュー 社. 200 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagashima T: "Growth factors and neurotrophic factors with relevance to gliomas"Brain and Nerve. 49. 214-226 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagashima T, Matsuno A, Murakami M: "Chemotherapy for gliomas and their drug resistance"Annual Review -Nervous system-. 38. 812-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S, Nagashima T, Hori T: "In vitro inhibition of binding of tumor necrosis factor (TNF)-alpha by mono-clonal antibody to TNF receptor on glioma cell and monocyte"Neurologia medico-chirurgica. 38. 812-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuno A, Ohsuga Y, Utsunomiya H, Taketoshi S, Munakata S, Nagao K, Osamura RY, Tamura A, Nagashima T: "An improved ultrastructural double staining method of rat growth hormone and its mRNA using LR White resin : a technical note"Histochem J. 30. 105-109 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuno A, Nagashima T, Osamura RY, Watanabe K: "Application of ultrastructural in situ hybridization combined with immunohistochemistry to pathophysiological studies of pituitary cell : Technical Review"Acta Histochem Cytochem. 31. 259-265 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuno A et al: "An improved ultrastructural double-Staining method for rat growth hormone and its mRNA using White resin." Histochemical Journal. 30. 105-109 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuno A et al: "Application of ultrastructural in site hybridization combined with immurohistochemistry to pothophysiological Studies." Acta Histochem.Cytochem.31. 259-265 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上峰子他: "粟粒結核に続発した頭蓋内結核腫の1例" 脳神経外科 ジャーナル. 7. 235-239 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上峰子他: "脳腫瘍の頭痛" 診断と治療. 86. 905-908 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鳥海 正明 他: "急速な視力低下を契機に診断された巨大な壊死下垂体腺腫の一例" ACTH RELATED PEPTIDES. 9. 253-260 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka S et al: "In vitro Inhibition of binding of turnor necrosis factor[TNF]-2α by monoclonal antibody to TNF receptor on glioma cell and monocyte." Neurologia medico-chirurgica. 38. 812-818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 長島正他(分担): "Annual Reviw 神経 1998" 中外医学者, 324 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 長島正(分担): "前頭葉・頭頂葉[機能・解剖・手術]" メジカル ビュー社, 200 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 長島 正: "グリオーマの成長・栄養因子" 脳と神経. 49・3. 214-226 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 長島 正: "グリオーマの化学療法と薬剤耐性" Annual Review神経. 1998. 148-159 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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