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Invasiveness of malignant glioma and lipoprotein receptor related protein

Research Project

Project/Area Number 09671459
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionFukuoka University

Principal Investigator

YAMAMOTO Masaaki  Fukuoka Univ., Sch.of Medicine, Lecturer, 医学部, 講師 (80240125)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsglioma / glioblastoma / invasiveness / LRP / uPA receptor / uPA / glioma / glioblastoma / invasiveness / 悪性神経膠腫 / 浸油能 / uPA Receptor
Research Abstract

Low density lipoprotein receptor-related protein (LRP) plays an important role in regulating proteinase activity, which is necessary for cellular invasive processes. In this study, we investigated the presence of both LRP and urokinase-type plasminogen activator receptor (uPAR) in astrocytoma tissues and in glioma cell lines by polymerase chain reaction and immunohistochemical analysis. LRP mRNA was frequently expressed in glioblastomas, as compared with low-grade astrocytomas by polymerase chain reaction analysis, and was correlated with uPAR expression. These results were consistent with the immunohistochemical localization of LRP protein in glioblastomas. Immunohistochemistry of LRP on sequential frozen sections showed that neoplastic glial cells and endothelial cells of glioblastomas exhibited intense LRP immunoreactivity, while LRP was almost undetectable in low-grade astrocytomas, or in normal glial cells and endothelial cells of normal brain tissues. In normal brain tissues, LRP immunoreactivity was identified in her places.ebral cortex. U105MG cell line, which did not express LRP, was transfected LRP cDNA.This LRP positive U1O5MG (U1O5MG LRP+) showed increased invasiveness compared with U105MG by Matrigel in vitro invasion assay. These results indicate that LRP is overexpressed in malignant astrocytomas, especially in glioblastomas, and the increased expression of LRP appears to correlate with the expression of uPAR and the malignancy of astrocytomas. Our results strongly suggest that LRP may play a role in facilitating glioblastoma invasiveness and neovascularization within tumor tissues by regulating cell surface proteolytic activity.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Masaaki Yamamoto et al.: "Increased expression of low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor in human..." Cancer Research. 57. 2799-2805 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masaaki Yamamoto, Kohichi Ikeda, Kohichi Ohshima, Hitoshi Tsugu, Hideo Kimura, Masamichi Tomonaga: "Increased expression of low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor in human malignat astrocytomas." Cancer Research. 57. 2799-2805 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masaaki Yamamoto,et.al: "Increased expression of low density lipoprotein recepta-related prrtein/α2・masroglobulin Receptor in human madliguant artrocextume" Concer Research. 57. 2799-2805 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaaki Yamamoto,et.al: "Expression and cellulen localigation of low dmsity topoprotein receptor rdated protein/α2-macroglobnlin receptor in human gliotlosties in vivo" Brain Yuma Pathology. (発表予定).

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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