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Apoptosis activated in fibrosarcoma growth

Research Project

Project/Area Number 09671471
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionGunma University

Principal Investigator

WATANABE Hideomi  Gunma Univ., Orthopedics, Lecturer, 医学部, 講師 (40231724)

Co-Investigator(Kenkyū-buntansha) TAKAGISHI Kenji  Gunma Univ., Orthopedics, Professor, 医学部, 教授 (70154763)
SHINOZAKI Tetsuya  Gunma Univ., Orthopedics, Assistant, 医学部, 助手 (90251115)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsApoptosis / Fibrosarcoma / Cell cycle
Research Abstract

A protein-independent fibrosarcoma, Gc-4 PF, grows exponentially in a protein-free medium. The doubling time was similar to that of the serum-dependent parental clone, Gc-4 SD cultivated in the presence of fetal calf serum. The protein-free cultivation of Gc-4 PF cells concomitantly activates apoptotic phenotypes (one third total cell population), including typical morphology, high uptake of Hoechst 33342 dye, and cleavage of DNA to large fragments, as observed in protein-deprived Gc-4 SD cell previously. Gc-4 SD cells arrested in the GィイD20ィエD2/GィイD21ィエD2 phase in response to the protein-free condition. In contrast, Gc-4 PF cells did not reach GィイD20ィエD2/GィイD21ィエD2 arrest in the protein-free condition ; instead the durations of both GィイD20ィエD2/GィイD21ィエD2 and GィイD22ィエD2 phases were markedly reduced. The estimation of one cell cycle duration revealed that the cell division cycle was accelerated to 1.7 -fold. Then the growth kinetics was able to be verified quantitatively by both the cell division rate and apoptotic cell loss. Protein-free cultivation resulted in slight down-regulation of c-myc protein in both cell types, while the down-regulation of P34ィイD1cdc2ィエD1, shown clearly in Gc-4 SD cells, was avoided in Gc-4 PF cells. Interestingly, while the expression of p53 was not affected in Gc-4 SD cells in response to the protein-free condition, the suppressor gene product expression was suppressed markedly in Gc-4 PF cells. From these results Gc-4 PF cells may have acquired an ability to accelerate cell division by shortening the cell cycle duration to maintain a proper growth rate in response to intrinsic apoptosis activation with, at least in part, a suppression of p53 expression as well as an escape of down-regulation of P34ィイD1cdc2ィエD1.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takeuchi et al.: "Biochemical investigation of a cell motile activity・・・・"J. Rheumatol.. 25. 9-15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe et al.: "Calcific tendinitis in the posterior provymal thigh・・・・"J. Rheumatol.. 25. 970-974 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Niinaka et al.: "Expression and secretion of neuroleukin/phospho・・・・"Cancer Res.. 58. 2667-2674 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinozaki et al.: "Allotype immunogloburin enhances・・・・"J. Lab. Clin. Med.. 132. 320-328 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe et al.: "Death activation does not stop tumor growth・・・・"Int. J. Oncol.. 13. 807-818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe et al.: "Glucose metabolism analysis of・・・・"J. Bone Joint Surg.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeuchi, K et al.: "Biochemical investigation of a cell motile activity ewpressed in rheumatoid synovial fluid."J. Reumatol.. 25. 9-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe H et al.: "Calcific tendinitis in the posterior proximal thigh as a self-limited condition : Pathogenic role of inflammatory responses."J. Rheumatol. 25. 970-974 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Niinaka Y et al.: "Expression and secretion of neuroleukin/phosphohexose isomerase/maturation factors as autocrine motility factor by tumor cells."Cancer Res.. 58. 2667-2674 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinozaki T et al.: "Allotype immunoglobulin enhances alkaline phosphatase activity : Implications for the inflammatory response."J. Lab. Clin. Med.. 132. 320-328 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe H, et al.: "Death activation does not stop tumor growth : Quantitative evidnce for concomitant activation of apoptosis in tumor growth in vitro."Int. J. Oncol.. 13. 807-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe H, et al.: "Glucose metabolic analysis of musculoskeletal tumors using Fluorine-18-PDG PET as an aid to preoperative planning."J. Bone Joint Surg. [Br]. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murata et al.: "Induction of autocrine factor inhibiting cell ..."Int.J.Cancer. 80. 889-895 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Aoki et al.: "FDG-DET in differential diognosis and ..."J.Comput.Assist.Tomography. 23. 603-608 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu et al.: "The autorcine motility factor receptor ..."FEBS Lett.. 456. 295-300 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe et al.: "Glucose metabolic analysis of ..."J. Bone Joint Surg.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawanishi et al.: "Correlation between loss of E-cadherin ..."Am.J.Clin.Pathol.. (in press). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taniguchi et al.: "The relation between the growth patterns of gashic carcinoma" Cancer. 82. 2112-2122 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shinozaki et al.: "Imaging features of subcntaneous sarcoidosis" Sleelctal Radiol.27. 359-364 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Niinaka et al.: "Expression and secretion of neuroleulcin/phospho." Cancer Res.58. 2667-2674 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takanami et al.: "Autocrine motility factor in pulmonary adenocarcinoma." Tumor Biol.19. 384-389 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shinozaki et al.: "Allotype immunogloburin euharces." J. Lab. Clin. Med.132. 320-328 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe et al.: "Death activatin does not stop tumar guowth." Int. J. Oncol.13. 807-818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi et al.: "Biochemical inveshigation of a cell morile adhivity expressed in." J.Rheumatol.25(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takayanagi et al.: "Overgvowih of the ischial tubevonity conplicating femoral." Am.J.Orthop.in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Watanabe et al.: "Calcific teudinitis in the posterior proxynal tbuigh." J.Rheumatol.25(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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