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vascularized osteo-periosteal grafts prefabricated by applying BMP on the periosteum.

Research Project

Project/Area Number 09671483
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionShinshu University

Principal Investigator

SAITOH Satoru  Shinshu University Department of Orthopaedics, lecturer, 医学部・附属病院(整形外科), 講師 (20175350)

Co-Investigator(Kenkyū-buntansha) TAKAOKA Kunio  Shinshu University Department of Orthopaedics, Professor, 医学部・整形外科, 教授 (30112048)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsbone morphogenetic protein / periosteum / vascularized tissue transfer / 骨成形因子 / 血管柄付き骨・骨膜移植 / 異所性骨形成
Research Abstract

The vascularized periosteal flap was raised on the medial side of the rat tibia in order to investigate the osteogenetic ability of the periosteum and the effect of a bone morphogentic protein (BMP) on its osteogenesis. Materials and Methods : The periosteum on the medical aspect of the rat tibia was anatomically dissected and the periosteum was found to be nourished by the saphenous artery and veins, which was one of the branches of the femoral vessels. The periosteum was disseced from the tibia under an operating microscope. The amount of the BMP implanted was 10 mug in total, and a newly developed, synthetic absorbable poly-D,L-lactic acid polyethylene glycol (PLA-PEG) block copolymers was used as a BMP-carrier. The rats were divided into four groups and the following procedures were performed in each group. The periosteum was just raised and the skin was closed immediately in Group 1. The periosteum was raised and the PLA-PEG not containing the BMP was implanted between the periost … More eum and tibia in Group 2. The periosteum was raised and the PLA-PEG containing the BMP was placed between the periosteum and tibia in Group 3. The PLA-PEG containing the BMP was implanted on the periosteum in Group 4. The tibia was removed three weeks postoperatively and the amount of the new bone formation was examined histologically and by radiographs as well. Results : A small amount of new bone was seen histologically in Group 1 and 2. A definite and relatively a large amount of new bone was formed in Group 3 along with a periosteal thickening. In Group 4, on the other hand, there was little bone formation or periosteal thickening. Conclusions : The periosteum proved to have an ability to generate bone tissue after a periosteal stripping alone. The amount of bone formed was, however, small and appeared not enough to be transferred into bony defects. The periosteum stimulated by the BMP implanted between the tibia and periosteum exhibited an extensive osteogenesis in three weeks. The bone formed by the BMIP-stimulated periosteum appeared good enough to be transferred as a vascularized prefabricated osteo-periosteal graft, which was to be raised on the saphenous or femoral vessels and grafted as a pedicled or free graft into a bony defect in the femur or tibia. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 斉藤直人,高岡邦夫: "骨形成因子(BMP)の担体として機能する生体材料" 整形外科. 48・8. 1133-1137 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 斉藤直人,高岡邦夫: "骨形成因子(BMP)の担体の複合による骨組織の再生" 生体材料. 15. 311-313 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saitoh S,Takaoka K et al: "Peroneal osteocutaneous flap raised on reconstructed popliteal artery for delayed union following open fibial fractures" Microsurgery. 17. 230-237 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arai N,Saitoh S,Seki H,Takaoka K: "Long-term result of arterial grafts interposed for arterial defeets using the telescoping anastomotic technique" Microsurgerg. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 村上成道,斉藤 覚,高岡邦夫 ほか: "hr BMP-2を利用した骨膜の骨形成能の検討" 日本手の外科学会誌. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka K,Saito N,et al: "Tissue Engineering for therapeutic use" Elsevier Science BV,Amsterdam in press, 11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka K,Miyasono S,Saito N,Okada T: "Biomaterials engineering and devices;human applications" The Humana Press,New Jersey in press,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nurakami N,Saitoh S,Seki H,Saito N,Takaoka K,Hata Y.: "Enhancement of periosteal osteogenesis by application of the BMP." Journal of Japanese Society for Surgery of the Hand. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saitoh S,Matsuda S,Nakatsuchi Y,Takaoka K.: "Peroneal osteocutaneous flap raised on reconstructed popliteal artery for delayed union following open tibial fractures." Microsurgeru. 17. 230-237 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arai N,Saitoh S,Seki H,Takaoka K.: "Long-term result of arterial grafts interposed for arterial defects using the telescoping anastomotic technique : histological and angiographic study" Microsurgery. 19 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka K,Saito N,Miyazono S,Yoshikawa H,Okada T.: Bone-inducing implants : new asynthetic absorbable poly-D,L-lactic acid polyethylene glycol block copolymers as BMP-carriers.in Ikeda Y,Yamaoka Y ed.Tissue Engineering for Therapeutic Use 3.Elsevier Science, Amsterdam, (in press), (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka K,Miyazono S,Saito N,Okada T.: New synthetic polymers as carrier materials for BMP.in Wise DL ed.Biomaterials engineering and devices : human applications. The Humana Press, New Jersey, (in press), (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 斉藤直人,高岡邦夫: "骨形成因子(BMP)の担体として機能する生体材料" 整形外科. 48・8. 1133-1137 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 斉藤直人,高岡邦夫: "骨形成因子(BMP)の担体の複合による骨組織の再生" 生体材料. 15. 311-313 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saitoh S, Takaoka K, et al: "Peroneal osteo cataneous flap raised on recoustructed popliteal artery for delayed unon following open tibial fractures" Microsurgery. 17. 230-237 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Atai N, Saitoh S, Seki H, Takaoka K: "Long-term result of arterial grafts interposed for arterial defects using the telescoping anastomotic technique" Microsurgery. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 村上成道,斉藤覚,高岡邦夫ほか: "hr BMP-2を利用した骨膜の骨形成能の検討" 日本手の外科学会誌. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaoka K, Saito N, et al: "Tissue Engineering for therapeutic use" Elsevier Science BV, Amsterdam (in press), 11

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaoka K, Miyasono S, Saito N, Okada T: "Biomaterials engineering and devices ; human applications" The Humana Press, New Jersey (in press),

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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