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Cell Kinetic of soft tissue sarcoma under the treatment of anti-cancer agents

Research Project

Project/Area Number 09671486
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionSHIGA UNIVERSITY OF MEDICAL SCIENCE

Principal Investigator

MATSUMOTO Keiji  Shiga University of Medical Science, Dept. of Orthopaedic Surgery, Assistant Professor, 医学部, 講師 (70165885)

Co-Investigator(Kenkyū-buntansha) ISHIZAKA Michihito  Shiga University of Medical Science, Dept. of Orthopaedic Surgery, Instructor, 医学部, 助手 (00242983)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Keywordscell kinetics / bromodeoxyuridine (BrdU) / iododeoxyuridine (IUdR) / soft tissue sarcoma / spoptosis / ヌードマウス / 多剤耐性遺伝子 / 骨軟部腫瘍 / ネクローシス
Research Abstract

To clarify in the vivo effects and the changes in kinetic parameters of soft tissue sarcoma caused by anticancer agents, we performed double-labeling using bromo- (BrdU) and iododeoxyuridine (IUdR). A malignant fibrous histiocytoma cell line was transplanted into nude mice, and treated with 3 anticancer agents : cisplatin, doxorubicin and vincristine. The following parameters were determined : BrdU labeling index (LI), duration of S-phase (Ts), total cell cycle time (Tc), Ki-67 labeling index (KLI), PCNA labeling index (PCNA-LI), and actual tumor volume doubling time (Tv). In addition, the apoptotic cell ration (apoptotic index ; AI) was calculated in the serial specimens using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL).
Vincristine inhibited the tumor growth the most effectively (Tv : -5.8 days). Doxorubicin suppressed the tumor growth better (Tv 12.7 days) than cisplatin (Tv : 9.1 days). TC values showed significant differences among the drug groups (ANOVA analysis ; p<0.05), and also correlated with Tv (Pearson's analysis ; R>0.7, p<0.05) on Day 1. AI peaked on day 10 and decreased afterward in every drug group.
Anticancer agents effectively suppress tumor growth not only by necrosis and apoptosis, but also by prolonged Tc. Tc values are possibly useful for planning chemotherapy for sarcoma patients.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Egawa M, Chano T, Matsumoto K, et al.: "Kinetic analysis of malignant fibrous histioeytoma cells treated with anticancer agents in vivo"Acta Histochem Cytochem. 32. 437-443 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Meyn,R. E., Stephens, L.C., Hunter, N.R., and Milas, L.: "Apoptosis in murine tumors treated with chemotherapy agents"Anticancer Drugs. 6. 113-450 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morimoto, S., Matsumoto, K., Chano, T., Ishizawa, M., Hukuda, S., and Okabe, H.: "Kinetic analysis of tumor cells in bone and soft tissue sarcomas"Acta. Histochem. Cytochem.. 30. 203-210 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mundle, S., Iftikhar, A., Shetty, V., Dameron, S., Wright Quinones, V., Marcus, B., Loew, J., Gregory, S. and Raza, A.: "Novel in situ double labeling for simultaneous detection of proliferation and apoptosis"J. Histochem. Cytochem.. 42. 1533-1537 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ovejera, A. A., and Houchens, D. P.: "Human tumor xenografts in athymic nude mice as a preclinical screen of anticancer agents"Semin. Oncol.. 8. 386-393 (1981)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yonezawa, M., Otsuka, T. and Matsui, N.: "Hyperthermia induces apoptosis in malignant fibrous histiocytoma cells in vitro"Int. J. Cancer. 66. 347-351 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Egawa M,Chano T,Matsumoto K et al.: "Kinetic analysis of malignant fibrous histiocytoma cells treated with anticancer agents in vivo"Acta Histochem Cytochem. 32. 437-443 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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