| Project/Area Number |
09671493
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University Faculty of Medicine |
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Principal Investigator |
YONENOBU Kazuo Osaka University Faculty of Medicine, Associate professor, 医学部, 助教授 (50127320)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Kozo Osaka University Faculty of Medicine, Assistant professor, 医学部, 助手 (00216061)
WADA Eiji Osaka University Faculty of Medicine, Assistant professor, 医学部, 助手 (40273643)
ARAKI Toshiyuki Osaka University Faculty of Medicine, Assistant professor, 医学部, 助手 (70263275)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | peripheral nerve / nerve regeneration / Schwann cell / adhesion / ninjurin |
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| Research Abstract |
During peripheral nerve regeneration, Schwann cells generate a number of factors which are necessary for nerve regeneration, which is a distinctive characteristics of peripheral nervous system.We have found plasma membrane proteins, ninjurin-1, by virtue of its up-regulation in Schwann cells after nerve injury, and ninjurin-2 by its homology with ninjurin1.Ninjurin-2 expression in the normal adult organs was primarily found in hematopoietic and lymphatic organs.In the peripheral nervous system, ninjurin-2 expression was found mostly in neurons of the peripheral ganglia including dorsal root and trigeminal ganglia, which showed a good contrast to ninjurin-1 exression observed only in satellite cells.After peripheral nerve injury, ninjurin-1 and -2 are both up-regulated in the neurons 1-3 days after injury.In the Schwann cells, both ninjurin-1 and -2 are hardly expressed in the normal myelinating Schwann cells, and dramatically up-regulated after nerve injury.Furthermore, we have found that neurite outgrowth from primary cultured DRG neurons is promoted when they are grown on ninjurin-2-overexpressing CHO cells.These results indicate that ninjurin-2 may functions differently from ninjurin-1 in normal peripheral nervous system, but after injury both ninjurin1 and ninjurin-2 promote nerve regeneration by their homophilic adhesion properties.
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