Experimental Study of Artificial Tendon Sheath Using Synovial Cell and Collagen Membrane
| Project/Area Number |
09671498
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MOCHIZUKI Yu Hiroshima University Faculty of Medicine, Research Associate, 医学部, 助手 (10284192)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Tendon / Tendon sheath / Epitenon / Synovial cell / Fibroblast / Scar fomation / Adhesion / Culture |
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| Research Abstract |
When tendons were injured, massive scar fomation around the repaired tendon caused poor clinical results, such as limitation of range of motion, pain, swelling, etc. But there are few literature about the mechanism of scar formation. Scar formation around the sutured tendon and the role of paratenon and epitenon were investigated histologically in this study.
Bilateral Achilles! tendons of rabbits were cut and sutured. On the right side, paratenon and epitenon were repaired (repaired group) , and on the left side, they were resected (resected group). Scar formation around the sutured tendon was investigated histologically at 1, 2, 3, 4 weeks after the operation. At I week, fibroblasts and loose connective tissue were recognized in both groups. They were dominant in resected group than in repaired group. At 2 week, vascuralization and cellular infiltration were dominant in resected group than in repaired group. At 3 weeks, the amount of collagen fiber has increased around the sutured tendon much more in resected group than in repaired group. At 4 weeks, there was no remarkable difference with at 3 weeks. Therefore, repairing of paratenon and epitenon is important to reduce scar formation.
Our future study project is the development of artificial tendon sheath. We will culture fibroblasts from paratenon and epitenon on collagen membrane, and transport them around the sutured tendon. If this procedure will be established, it will contribute to the clinical result after tendon and ligament surgery.
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Report
(3 results)
Research Products
(3 results)
