| Project/Area Number |
09671499
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TAKEDA Yoshitsugu The University of Tokushima, School of Medicine University Hospital, Assistant, 医学部附属病院, 助手 (20243694)
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| Co-Investigator(Kenkyū-buntansha) |
UMEFARA Takashi The University of Tokushima, School of Medicine Assistant, 医学部, 助手 (20263816)
KATOH Shinsuke The University of Tokushima, School of Medicine Lecturer, 医学部, 講師 (30243687)
KASHIWAGUCHI Shinji The University of Tokushima, School of Medicine University Hospital, Lecturer, 医学部附属病院, 講師 (30224398)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | articular cartilage / growth plate / repair / apoptosis / MHC / transplantation / 成長期関節軟骨 / 関節軟骨修復 / DNA電気泳動 |
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| Research Abstract |
Transplants of the apophyseal growth plate obtained from the ilium of Lewis rats at 4 weeks of age were inserted into the full-thickness defects in the patellofemoral articular surface of Fisher, LEC and LEA rats. Lewis rat and Fisher rat have the same Major Histocompatibility Antigen (MHC), and MHC is different between Lewis rat and LEC / LEA rats. LEC rat and LEA rat possesses the same MHC, but LEC rat lack the CD4_+ T-cell function congenitally. Femoral specimens including transplants were cut and fixed in formarin and paraffin embedded. The tissue sections were studied by in situ end-labeling procedure at one-week intervals up to 4 weeks in order to detect the apoptosis.
In each recipient, no staining of cells with morphological characteristics of apoptosis was demonstrated in growth plate until the incorporation of the grafts to the defects. But apoptotic cells were detected in bone marrow under the graft at 1 and 2 weeks after transplantation. These findings were similar to that of autografts.
The results of this study suggested that the immunological response of the recipients do not affct on the apoptosis that has been developed in the donor site by allograft of the apophyseal growth plate into the full-thickness defects in the patellofemoral articular surface.
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