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Establishment of experimental model by using gene transfer and Leukemia Inhibitory Factor (LIF) "knock-out" mouse

Research Project

Project/Area Number 09671502
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

FUJII Tohru  Nagasaki University School of Medicine Professor, 医学部, 教授 (60136661)

Co-Investigator(Kenkyū-buntansha) AKITA Sadanori  Nagasaki University School of Medicine Hospital Staff Physician, 医学部附属病院, 医員
YAMANOBE Yuuji  Nagasaki University School of Medicine Hospital Assistant, 医学部附属病院, 助手 (40284690)
HIRANO Akiyoshi  Nagasaki University School of Medicine Assistant Professor, 医学部, 助教授 (90208835)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsLeukemia Inhibitory Factor (LIF) / Wound healing / immunity in grafting / signal transduction / 動物モデル
Research Abstract

The pituitary specific leukemia inhibitory factor (LIF) transgenic mouse revealed the significant morphological and molecular changes, which is expressed by the systemic dwarfism and the hormone producing cells in pituitary altered.
The growth hormone secreting cell implantation in the adult rats resulted in the positive correlation between mandibular bone augmentation and strong LIF expression shown by in situ hybridyzation.
The keloid-devived fibroblast cell culture study showed the IGF-1 receptor was specifically expressed among the tyrosine-type receptor subtypes and IGF-l was able to regulate the invasiveness of the keloid-derived fibroblast cells.
This may explain that the IGF-l and its receptor pathway markedly related to the keloid mechanism and signal transduction.
[IF is highly expressed in patients with psoriasis and contact dermatitis and its over-expression in local skin tissue could prolong the allogeneic skin grafting at 12, 24 and 72 hours after skin grafting on mouse back l.5x1.5 cm in between B6D2F1 and BALB/c strains. LIF Over-expressed allogeneic skin grafting resulted in the immune tolerance. This immune tolerance was regulated by LIF expressiona and and expressions of subsequent proper LIF receptor and signal transducing component namely gpl3O.Classical helper T cell expression such as Thl by IL-2 and Th2 by IL-10 were not detected. Thus this phenomena may be LIF specific and LlF may be most important regulator for the prolonged.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Akita S.,et.al.: "Pituitary-directed leukemia inhibitory factor transgene forms Rathke's cleft cysts and impairs adult pituitary function." J Clin Invest. 99. 2462-2469 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo K.,Tanaka K.,Fujii T.,and Akita S.: "Klippel-Trenaunay-Weber syndrome associated with intra-abdominal lymphangioma requiring multiple surgical intervention." Ann Plas Surg. 39(4). 435-437 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tohru Fujii,et al.: "Maxillary growth following atelocollagen implantation on mucoperiosteal denudation of palatal process in young rabbits considering of clinical cleft palate repair." Cleft Palate-Craniofacial journal.34(4). 297-308 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii Tohru,et al.: "Migration of Hydroxyatatite Onlays into the Mandible and Nasal Bone and Local Bone Turnover in Growing Rabbits." Plastic & Reconstructive Surgery.99(7). 1972-1982 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 平野 明喜et.al.: "頭蓋底・上部頚椎の腫瘍に対する上下顎正中分割によるアプローチ" 形成外科. 40・10. 991-996 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 平野 明喜: "顔面骨の骨切り術" 形成外科. 40. 59-69 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano A,et al.: "Tulip flap for reconstruction of the central tubercle in cleft lip patients" Plast Reconstr Surg. 101. 1345-1348 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujioka M,et al.: "Comparative study of inferior alveolar disturbance restoration after sagittal split osteotomy by means of bicortical versus monocortical osteosynthesis" Plast Reconstr Surg. 102. 37-41 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimoto H,et al.: "Increased proliferative activity of osteoblasts in congenital hemifacial Hypertrophy" Plast Reconstr Surg. 102. 1605-1610 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshimoto H,et al.: "Overexpression of insulin-like growth factor-1(IGF-1)receptor and the invasiveness of cultured keloid fibroblasts" Am J Pathol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rashid MA,et al.: "Co-administration of Basic Fibroblast Growth Factor and Sucrose Octasulfate(Sucralfate)Facilitates the Rat Dorsal Flap Survival and Viability" Plast Reconstr Surg. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 秋田定伯 他: "皮膚移植における白血病抑制因子(Leukemia Inhibitory Factor,LIF)の役割" Progress in Medicine. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akita S.et al.: "Pituitary-directed leukemia inhibitory factor transgene forms Rathke's cleft cysts and impairs adult pituitary function." J Clin Invest. 99. 2462-2469 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo K, Tanaka K, Fujii, T and Akita S.: "Klippel-Trenaunay-Weber syndrome associated with intra-abdominal lymphanjioma requiring multiple surgical intervention." Ann Plast Surg. 39. 435-437 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Maxillary growth following atelocollagen implantation on mucoperiosteal denudation of palatal process in young rabbits considering of clinical cleft palate repair." Cleft Palate-Cran J. 34. 297-308 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Migration of hydroxyapatite onlays into the mandible and nasal bone and local bone turnover in growing rabbits." Plast Reconstr Surg. 99. 1972-1982 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano A et al.: "Maxillo-mandibular splitting approach for cranial base and upper cervical spine tumors (Japanese)" Jpn J Plast Reconstr Surg. 40. 991-996 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano A.: "Facial bone osteotomy (Japanese)" Jpn J Plast Reconstr Surg. 40. supple. 59-69 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano A et al.: "Tulip flap for reconstruction of the central tubercle in cleft lip patients." Plast Reconstr Surg. 101. 1345-1348 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Comparative study of inferior alveolar disturbance restoration after sagittal split osteotomy by means of bicortical versus monocortical osteosynthesis." Plast Reconstr Surg. 102. 37-41 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Increased proliferative activity of osteoblasts in congenital hemifacial hypertrophy." Plast Reconstr Surg. 102. 1605-1610 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Overexpression of insulin-like growth factor-1(IGF-1) receptor and the invasiveness of cultured keloid fibroblasts." Am J Pathol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii T et al.: "Co-administration of basic growth factor and sucrose octasulfate (sucralfate) facilitates the rat dorsal flap survival and viability." Plast Reconstr Surg. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akita S et al.: "Role of leukemia inhibitory factor (LIF) in skin grafting(Japanese)." Progress in medicine. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano A,et al.: "Tulip flap for reconstruction of the central tubercle in cleft lip patients" Plast Reconstr Surg. 101. 1345-1348 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujioka M,et al.: "Comparative study of inferior alveolar disturbance restoration after sagittal split osteotomy by means of bicortical versus monocortical osteosynthesis" Plast Reconstr Surg. 102. 37-41 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimoto H,et al.: "Increased proliferative activity of osteoblasts in congenital hemifacial Hypertrophy" Plast Reconstr Surg. 102. 1605-1610 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimoto H,et al.: "Overexpression of insulin-like growth factor-1(IGF-1)receptor and the invasiveness of cultured keloid fibroblasts" Am J Pathol. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Rashid MA,et al.: "Co-administration of Basic Fibroblast Growth Factor and Sucrose Octasulfate(Sucralfate)Facilitates the Rat Dorsal Flap Survival and Viability" Plast Reconstr Surg. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 秋田定伯他: "皮膚移植における白血病抑制因子(Leukemia Inhibitory Factor,LIF)の役割" Progress in Medicine. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Akita S.,et.al: "Pituitary-directed leukemia inhibitory factor transgene forms Rathke's cleft cysts and impairs adult pituitary function." J Clin Invest. 99. 2462-2469 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kondo K.,Tanaka K.,Fujii T.,and Akita S.: "Klippel-Trenaunay-Weber syndrome associated with intra-abdominal lymphangioma requiring multiple surgical intervention." Ann Plas Surg. 39(4). 435-437 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tohru Fujii, et al.: "Maxillary growth following atelocollagen implantation on mucoperiosteal denudation of palatal process in young rabbits considering of clinical cleft palate repair." Cleft Palate-Craniofacial journal. 34(4). 297-308 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujii Tohru, et al.: "Migration of Hydroxyapatite Onlays into the Mandible and Nasal Bone and Local Bone Turnover in Growing Rabbits." Plastic & Reconstructive Surgery.99(7). 1972-1982 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平野 明喜et. al.: "頭蓋底・上部頚椎の腫瘍に対する上下顎正中分割によるアプローチ" 形成外科. 40・10. 991-996 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平野 明喜: "顔面骨の骨切り術" 形成外科. 40. 59-69 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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